{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T00:53:55Z","timestamp":1778892835371,"version":"3.51.4"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2021,8,15]],"date-time":"2021-08-15T00:00:00Z","timestamp":1628985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"National Institute of Health","award":["R01 ES029448"],"award-info":[{"award-number":["R01 ES029448"]}]},{"name":"National Institute of Health","award":["P30CA11800"],"award-info":[{"award-number":["P30CA11800"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Epidemiology studies link cigarillos and shisha tobacco (delivered through a hookah waterpipe) to increased risk for cardiopulmonary diseases. Here we performed a comparative chemical constituent analysis between 3 cigarettes, 3 cigarillos, and 8 shisha tobacco products. The potency for genotoxicity and oxidative stress of each product\u2019s generated total particulate matter (TPM) was also assessed using immortalized oral, lung, and cardiac cell lines to represent target tissues. Levels of the carcinogenic carbonyl formaldehyde were 32- to 95-fold greater, while acrolein was similar across the shisha aerosols generated by charcoal heating compared to cigarettes and cigarillos. Electric-mediated aerosol generation dramatically increased acrolein to levels exceeding those in cigarettes and cigarillos by up to 43-fold. Equivalent cytotoxic-mediated cell death and dose response for genotoxicity through induction of mutagenicity and DNA strand breaks was seen between cigarettes and cigarillos, while minimal to no effect was observed with shisha tobacco products. In contrast, increased potency of TPM from cigarillos compared to cigarettes for inducing oxidative stress via reactive oxygen radicals and lipid peroxidation across cell lines was evident, while positivity was seen for shisha tobacco products albeit at much lower levels. Together, these studies provide new insight into the potential harmful effects of cigarillos for causing tobacco-associated diseases. The high level of carbonyls in shisha products, that in turn is impacted by the heating mechanism, reside largely in the gas phase which will distribute throughout the respiratory tract and systemic circulation to likely increase genotoxic stress.<\/jats:p>","DOI":"10.1093\/toxsci\/kfab101","type":"journal-article","created":{"date-parts":[[2021,8,12]],"date-time":"2021-08-12T11:23:15Z","timestamp":1628767395000},"page":"67-82","source":"Crossref","is-referenced-by-count":6,"title":["Comparative Genotoxicity and Mutagenicity of Cigarette, Cigarillo, and Shisha Tobacco Products in Epithelial and Cardiac Cells"],"prefix":"10.1093","volume":"184","author":[{"given":"Carmen S","family":"Tellez","sequence":"first","affiliation":[{"name":"Lung Cancer Program, Lovelace Respiratory Research Institute, Albuquerque, NM, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel E","family":"Juri","sequence":"additional","affiliation":[{"name":"Lung Cancer Program, Lovelace Respiratory Research Institute, Albuquerque, NM, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Loryn M","family":"Phillips","sequence":"additional","affiliation":[{"name":"Lung Cancer Program, Lovelace Respiratory Research Institute, Albuquerque, NM, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kieu","family":"Do","sequence":"additional","affiliation":[{"name":"Lung Cancer Program, Lovelace Respiratory Research Institute, Albuquerque, NM, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cindy L","family":"Thomas","sequence":"additional","affiliation":[{"name":"Lung Cancer Program, Lovelace Respiratory Research Institute, Albuquerque, NM, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Randy","family":"Willink","sequence":"additional","affiliation":[{"name":"Lung Cancer Program, Lovelace Respiratory Research Institute, Albuquerque, NM, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendy W","family":"Dye","sequence":"additional","affiliation":[{"name":"Lung Cancer Program, Lovelace Respiratory Research Institute, Albuquerque, NM, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guodong","family":"Wu","sequence":"additional","affiliation":[{"name":"Lung Cancer Program, Lovelace Respiratory Research Institute, Albuquerque, NM, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Zhou","sequence":"additional","affiliation":[{"name":"Lung Cancer Program, Lovelace Respiratory Research Institute, Albuquerque, NM, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hammad","family":"Irshad","sequence":"additional","affiliation":[{"name":"Lung Cancer Program, Lovelace Respiratory Research Institute, Albuquerque, NM, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shosei","family":"Kishida","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry and Genetics, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tohru","family":"Kiyono","sequence":"additional","affiliation":[{"name":"Exploratory Oncology Research & Clinical Trial Center, National Cancer Center, Chiba, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1240-2504","authenticated-orcid":false,"given":"Steven A","family":"Belinsky","sequence":"additional","affiliation":[{"name":"Lung Cancer Program, Lovelace Respiratory Research Institute, Albuquerque, NM, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,8,15]]},"reference":[{"key":"2021103112181795300_kfab101-B1","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/1617-9625-5-16","article-title":"Perceptions of hookah smoking harmfulness: predictors and characteristics among current hookah users","volume":"5","author":"Aljarrah","year":"2009","journal-title":"Tob. 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