{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T03:39:57Z","timestamp":1768534797978,"version":"3.49.0"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T00:00:00Z","timestamp":1651017600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"FDA Center for Drug Evaluation and Research"},{"name":"National Center for Toxicological Research"},{"name":"U.S. Department of Energy and FDA"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,6,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been widely used in the Comprehensive in vitro Proarrhythmia Assay (CiPA). The notable difference of the electrophysiological (EP) responses of hiPSC-CMs in serum and serum-free media (SFM) is puzzling and may impact regulatory decision-making on the cardiac safety of candidate drugs in inducing QT prolongation and torsade de pointes (TdP). In this study, we compared the EP responses of hiPSC-CMs to 10 CiPA compounds and moxifloxacin in serum and SFM; explained the potential reason behind the different EP responses\u2014abiotic compound loss to plastic tubes\/plates of hydrophobic compounds prepared in SFM; and investigated the impact of compound preparation methods on drug bioavailability in exposure media, which affects the TdP risk prediction of drugs tested in serum-containing and SFM. For assays to be conducted in SFM, awareness of abiotic compound loss of hydrophobic compounds in serum-free preparations is critical for delay repolarization evaluation and data extrapolation from in vitro to in vivo.<\/jats:p>","DOI":"10.1093\/toxsci\/kfac043","type":"journal-article","created":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T19:10:39Z","timestamp":1650654639000},"page":"48-61","source":"Crossref","is-referenced-by-count":6,"title":["Effects of Serum and Compound Preparation Methods on Delayed Repolarization Evaluation With Human iPSC-CMs"],"prefix":"10.1093","volume":"188","author":[{"given":"Feng","family":"Wei","sequence":"first","affiliation":[{"name":"Division of Systems Biology, National Center for Toxicological Research, U.S. Food and Drug Administration , Jefferson,\u00a0Arkansas 72079, USA"},{"name":"Department of Structural Heart Disease, the First Affiliated Hospital of Xi\u2019an Jiaotong University , Xi\u2019an, Shaanxi 710061, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Pence","sequence":"additional","affiliation":[{"name":"Division of Systems Biology, National Center for Toxicological Research, U.S. Food and Drug Administration , Jefferson,\u00a0Arkansas 72079, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kellie","family":"Woodling","sequence":"additional","affiliation":[{"name":"Division of Biochemical Toxicology, National Center for Toxicological Research, U.S. Food and Drug Administration , Jefferson,\u00a0Arkansas 72079, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prathyusha","family":"Bagam","sequence":"additional","affiliation":[{"name":"Division of Systems Biology, National Center for Toxicological Research, U.S. Food and Drug Administration , Jefferson,\u00a0Arkansas 72079, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Beger","sequence":"additional","affiliation":[{"name":"Division of Systems Biology, National Center for Toxicological Research, U.S. Food and Drug Administration , Jefferson,\u00a0Arkansas 72079, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Goncalo","family":"Gamboa da Costa","sequence":"additional","affiliation":[{"name":"Office of the Director, National Center for Toxicological Research, U.S. Food and Drug Administration , Jefferson,\u00a0Arkansas 72079, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Pang","sequence":"additional","affiliation":[{"name":"Division of Systems Biology, National Center for Toxicological Research, U.S. Food and Drug Administration , Jefferson,\u00a0Arkansas 72079, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,4,27]]},"reference":[{"key":"2022081807574828800_kfac043-B1","doi-asserted-by":"crossref","first-page":"178","DOI":"10.3389\/fcell.2020.00178","article-title":"A brief review of current maturation methods for human induced pluripotent stem cells-derived cardiomyocytes","volume":"8","author":"Ahmed","year":"2020","journal-title":"Front. 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