{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T16:02:53Z","timestamp":1782057773349,"version":"3.54.5"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,2,17]],"date-time":"2024-02-17T00:00:00Z","timestamp":1708128000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"JSPS KAKENHI","award":["20K18978"],"award-info":[{"award-number":["20K18978"]}]},{"name":"JSPS KAKENHI","award":["23K16373"],"award-info":[{"award-number":["23K16373"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Organophosphorus pesticides (OPPs) having a phosphate ester moiety, such as malathion (MA) and methidathion (DMTP), are widely used and have been detected in many fatal cases of accidental exposure or suicide in Japan. In forensic toxicology, the accurate determination of blood OPP concentration is mandatory to prove death by OPP poisoning. However, fatal pesticide concentration in blood at autopsy varies depending on the circumstances surrounding the dead body. In this study, we found that 16 OPPs were degraded by human serum albumin (HSA) in a temperature-dependent fashion. The mechanism underlying MA, DMTP, azinphos-methyl, etrimfos, fenthion (MPP), pirimiphos-methyl, (E)-dimethylvinphos, (Z)-dimethylvinphos, vamidothion, edifenphos (EDDP), fosthiazate, and pyraclofos degradation involves the formation of adducts with tyrosine residues in HSA. The mass spectra obtained by liquid chromatography quadrupole Orbitrap mass spectrometry revealed that phosphate ester amino acid adducts such as Y-adduct1, Y-adduct2, Y-adduct3, Y-adduct4, and Y-adduct5 were formed in HSA solution incubated with OPPs. These results indicate that the 16 OPPs are postmortem changed by HSA. The detection of phosphate ester amino acid adducts such as Y-adduct1, Y-adduct2, Y-adduct3, Y-adduct4, and Y-adduct5, instead of MA, DMTP, azinphos-methyl, etrimfos, MPP, pirimiphos-methyl, (E)-dimethylvinphos, (Z)-dimethylvinphos, vamidothion, EDDP, fosthiazate, and pyraclofos per se, may be used to determine death by these OPPs poisoning.<\/jats:p>","DOI":"10.1093\/toxsci\/kfae023","type":"journal-article","created":{"date-parts":[[2024,2,17]],"date-time":"2024-02-17T11:55:06Z","timestamp":1708170906000},"page":"40-48","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of organophosphorus pesticide tyrosine adducts for postmortem change by human serum albumin with liquid chromatography quadrupole Orbitrap mass spectrometry"],"prefix":"10.1093","volume":"199","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6719-5186","authenticated-orcid":false,"given":"Yoshikazu","family":"Yamagishi","sequence":"first","affiliation":[{"name":"Department of Legal Medicine, Graduate School of Medicine, Chiba University , Chuo, Chiba 260-8670, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayaka","family":"Nagasawa","sequence":"additional","affiliation":[{"name":"Department of Legal Medicine, Graduate School of Medicine, Chiba University , Chuo, Chiba 260-8670, Japan"},{"name":"Laboratory of Forensic Toxicology, Graduate School of Pharmaceutical Sciences, Chiba University , Chuo, Chiba 260-8675, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirotaro","family":"Iwase","sequence":"additional","affiliation":[{"name":"Department of Legal Medicine, Graduate School of Medicine, Chiba University , Chuo, Chiba 260-8670, Japan"},{"name":"Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo , Tokyo 113-8654, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8268-1828","authenticated-orcid":false,"given":"Yasumitsu","family":"Ogra","sequence":"additional","affiliation":[{"name":"Department of Legal Medicine, Graduate School of Medicine, Chiba University , Chuo, Chiba 260-8670, Japan"},{"name":"Laboratory of Forensic Toxicology, Graduate School of Pharmaceutical Sciences, Chiba University , Chuo, Chiba 260-8675, Japan"},{"name":"Laboratory of Toxicology and Environmental Health, Graduate School of Pharmaceutical Sciences, Chiba University , Chuo, Chiba 260-8675, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,2,17]]},"reference":[{"key":"2024042916032381500_kfae023-B1","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1007\/s00414-018-1898-1","article-title":"Temperature: The weak point of forensic entomology","volume":"133","author":"Charabidze","year":"2019","journal-title":"Int. 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