{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T23:11:00Z","timestamp":1786403460170,"version":"build-2736575974"},"reference-count":59,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T00:00:00Z","timestamp":1743120000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01ES028201"],"award-info":[{"award-number":["R01ES028201"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21ES033532"],"award-info":[{"award-number":["R21ES033532"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008975","name":"University of Massachusetts Amherst","doi-asserted-by":"publisher","award":["T32-GM135096"],"award-info":[{"award-number":["T32-GM135096"]}],"id":[{"id":"10.13039\/100008975","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Developmental perfluorooctanesulfonic acid (PFOS) exposure in zebrafish reduces digestive gene expression and pancreas length, indicating exocrine insufficiency. This project focuses on the production and function of digestive proteases with PFOS exposure. We test the hypothesis that developmental PFOS exposure impairs exocrine pancreas function in the absence of severe morphological changes. Three larval timepoints were assessed, where the nutrient source varies (yolk feed at 4-d postfertilization [dpf], yolk depleted at 6 dpf, and exogenously fed at 9 dpf) to understand how nutrients were being used throughout exocrine pancreas development. Tg(ptf1a: GFP) zebrafish were exposed to 0 (0.01% DMSO), 1, 2, and 4\u2009\u03bcM PFOS from 0 to 4 dpf. At 4 dpf, pancreas length was decreased with 1\u00a0\u03bcM and yolk sac area was reduced with 2 and 4\u2009\u03bcM PFOS. By 6 dpf, pancreata of zebrafish exposed to 1\u2009\u03bcM PFOS had recovered, and pancreas size was decreased with 4\u2009\u03bcM PFOS. Protease activity was reduced with PFOS exposure, accompanied by decreases in digestive protease gene expression and trypsin protein. At 9 dpf, there was no measurable change in pancreas size or protease activity with 1 and 2\u2009\u03bcM PFOS, indicating morphological and functional recovery even though PFOS was detected in the larvae. This study demonstrates that PFOS exposure can affect the function of the exocrine pancreas in the absence of a detectable change in organ size. We also highlight the mishandling of yolk nutrients, leading to undernutrition at later larval stages and show catch-up growth in morphology and function.<\/jats:p>","DOI":"10.1093\/toxsci\/kfaf041","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T19:54:15Z","timestamp":1743450855000},"page":"46-57","source":"Crossref","is-referenced-by-count":2,"title":["Developmental perfluorooctanesulfonic acid exposure impairs exocrine pancreas function in zebrafish (<i>Danio rerio<\/i>)"],"prefix":"10.1093","volume":"206","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7128-5216","authenticated-orcid":false,"given":"Madeline C","family":"Tompach","sequence":"first","affiliation":[{"name":"Molecular and Cellular Biology Graduate Program, University of Massachusetts Amherst , Amherst, MA 01003,","place":["United States"]},{"name":"Biotechnology Training Program, University of Massachusetts Amherst , Amherst, MA 01003,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7807-948X","authenticated-orcid":false,"given":"Charlotte K","family":"Gridley","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Biology Graduate Program, University of Massachusetts Amherst , Amherst, MA 01003,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9293-0392","authenticated-orcid":false,"given":"Marjorie","family":"Marin","sequence":"additional","affiliation":[{"name":"Biotechnology Training Program, University of Massachusetts Amherst , Amherst, MA 01003,","place":["United States"]},{"name":"Department of Environmental Health Sciences, University of Massachusetts Amherst , Amherst, MA 01003,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4373-5902","authenticated-orcid":false,"given":"Patrick F","family":"Murphy","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Biology Graduate Program, University of Massachusetts Amherst , Amherst, MA 01003,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1922-3901","authenticated-orcid":false,"given":"Junghak","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts Amherst , Amherst, MA 01003,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M","family":"Clark","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts Amherst , Amherst, MA 01003,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8835-5038","authenticated-orcid":false,"given":"Alicia R","family":"Timme-Laragy","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Biology Graduate Program, University of Massachusetts Amherst , Amherst, MA 01003,","place":["United States"]},{"name":"Department of Environmental Health Sciences, University of Massachusetts Amherst , Amherst, MA 01003,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,3,28]]},"reference":[{"key":"2025062603522293700_kfaf041-B1","doi-asserted-by":"crossref","first-page":"588S","DOI":"10.1080\/07315724.2004.10719428","article-title":"The developmental origins of adult disease","volume":"23","author":"Barker","year":"2004","journal-title":"J Am Coll Nutr"},{"key":"2025062603522293700_kfaf041-B2","doi-asserted-by":"publisher","first-page":"G10","DOI":"10.1152\/ajpgi.00077.2010","article-title":"The islet-acinar axis of the pancreas: more than just insulin","volume":"299","author":"Barreto","year":"2010","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"2025062603522293700_kfaf041-B3","doi-asserted-by":"publisher","first-page":"152565","DOI":"10.1016\/j.tox.2020.152565","article-title":"Early life exposure to per- and polyfluoroalkyl substances (PFAS) and latent health outcomes: a review including the placenta as a target tissue and possible driver of peri- and postnatal effects","volume":"443","author":"Blake","year":"2020","journal-title":"Toxicology"},{"key":"2025062603522293700_kfaf041-B4","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1111\/j.1432-0436.1992.tb00680.x","article-title":"Immunohistochemical study of secretory proteins in the developing human exocrine pancreas","volume":"51","author":"Carrere","year":"1992","journal-title":"Differentiation"},{"key":"2025062603522293700_kfaf041-B6","doi-asserted-by":"publisher","first-page":"6387","DOI":"10.1021\/acs.est.6b06049","article-title":"Chlorinated polyfluoroalkyl ether sulfonic acids in matched maternal, cord, and placenta samples: a study of transplacental transfer","volume":"51","author":"Chen","year":"2017","journal-title":"Environ Sci Technol"},{"key":"2025062603522293700_kfaf041-B7","doi-asserted-by":"publisher","first-page":"9769","DOI":"10.1038\/s41467-024-54258-2","article-title":"Intestinal DHA-PA-PG axis promotes digestive organ expansion by mediating usage of maternally deposited yolk lipids","volume":"15","author":"Chen","year":"2024","journal-title":"Nat Commun"},{"key":"2025062603522293700_kfaf041-B9","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1002\/etc4890","article-title":"Per- and polyfluoroalkyl substance toxicity and human health review: current state of knowledge and strategies for informing future research","volume":"40","author":"Fenton","year":"2021","journal-title":"Environ Toxicol Chem"},{"key":"2025062603522293700_kfaf041-B10","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1016\/s0012-1606(03)00308-7","article-title":"Formation of the digestive system in zebrafish. II. Pancreas morphogenesis","volume":"261","author":"Field","year":"2003","journal-title":"Dev Biol"},{"key":"2025062603522293700_kfaf041-B11","doi-asserted-by":"publisher","first-page":"1317","DOI":"10.1016\/j.celrep.2016.01.016","article-title":"Zebrafish embryonic lipidomic analysis reveals that the yolk cell is metabolically active in processing lipid","volume":"14","author":"Fraher","year":"2016","journal-title":"Cell Rep"},{"key":"2025062603522293700_kfaf041-B12","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1111\/j.1432-0436.1986.tb00393.x","article-title":"Development of human pancreas","volume":"31","author":"Fukayama","year":"1986","journal-title":"Differentiation"},{"key":"2025062603522293700_kfaf041-B13","doi-asserted-by":"publisher","first-page":"47005","DOI":"10.1289\/EHP5843","article-title":"Evaluation of developmental toxicity, developmental neurotoxicity, and tissue dose in zebrafish exposed to GenX and other PFAS","volume":"128","author":"Gaballah","year":"2020","journal-title":"Environ Health Perspect"},{"key":"2025062603522293700_kfaf041-B14","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/S0091-679X(04)76024-0","article-title":"Pancreas development in zebrafish","volume":"76","author":"Gn\u00fcgge","year":"2004","journal-title":"Methods Cell Biol"},{"key":"2025062603522293700_kfaf041-B15","doi-asserted-by":"publisher","first-page":"5069","DOI":"10.1242\/dev.02075","article-title":"Targeting of amacrine cell neurites to appropriate synaptic laminae in the developing zebrafish retina","volume":"132","author":"Godinho","year":"2005","journal-title":"Development"},{"key":"2025062603522293700_kfaf041-B16","doi-asserted-by":"publisher","first-page":"684","DOI":"10.3390\/toxics10110684","article-title":"Reduced birth weight and exposure to per- and polyfluoroalkyl substances: a review of possible underlying mechanisms using the AOP-HelpFinder","volume":"10","author":"Gundacker","year":"2022","journal-title":"Toxics"},{"key":"2025062603522293700_kfaf041-B17","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/j.aquatox.2014.10.017","article-title":"PFOS affects posterior swim bladder chamber inflation and swimming performance of zebrafish larvae","volume":"157","author":"Hagenaars","year":"2014","journal-title":"Aquat Toxicol"},{"key":"2025062603522293700_kfaf041-B18","doi-asserted-by":"publisher","first-page":"133873","DOI":"10.1016\/j.chemosphere.2022.133873","article-title":"Concentrations of per- and polyfluoroalkyl substances (PFAS) in human placental tissues and associations with birth outcomes","volume":"295","author":"Hall","year":"2022","journal-title":"Chemosphere"},{"key":"2025062603522293700_kfaf041-B19","doi-asserted-by":"publisher","first-page":"G445","DOI":"10.1152\/ajpgi.90513.2008","article-title":"In vivo imaging of zebrafish digestive organ function using multiple quenched fluorescent reporters","volume":"296","author":"Hama","year":"2009","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"2025062603522293700_kfaf041-B20","doi-asserted-by":"publisher","first-page":"e1479","DOI":"10.1371\/journal.pone.0001479","article-title":"Genomewide expression analysis in zebrafish mind bomb alleles with pancreas defects of different severity identifies putative notch responsive genes","volume":"3","author":"Hegde","year":"2008","journal-title":"PLoS One"},{"key":"2025062603522293700_kfaf041-B21","doi-asserted-by":"publisher","first-page":"3416","DOI":"10.1210\/EN.2015-1394","article-title":"Developmental origins of health and disease: integrating environmental influences","volume":"156","author":"Heindel","year":"2015","journal-title":"Endocrinology"},{"key":"2025062603522293700_kfaf041-B22","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1097\/MOP.0000000000000191","article-title":"Developmental origins of health and disease: a paradigm for understanding disease cause and prevention","volume":"27","author":"Heindel","year":"2015","journal-title":"Curr Opin Pediatr"},{"key":"2025062603522293700_kfaf041-B23","doi-asserted-by":"publisher","first-page":"bio046839","DOI":"10.1242\/bio.046839","article-title":"Exocrine pancreas proteases regulate beta-cell proliferation in zebrafish ciliopathy models and in murine systems","volume":"10","author":"Hostelley","year":"2021","journal-title":"Biol Open"},{"key":"2025062603522293700_kfaf041-B24","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.aquatox.2010.02.003","article-title":"Toxicity, uptake kinetics and behavior assessment in zebrafish embryos following exposure to perfluorooctanesulphonicacid (PFOS)","volume":"98","author":"Huang","year":"2010","journal-title":"Aquat Toxicol"},{"key":"2025062603522293700_kfaf041-B25","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1016\/j.aquatox.2016.03.026","article-title":"PFOS, PFNA, and PFOA sub-lethal exposure to embryonic zebrafish have different toxicity profiles in terms of morphometrics, behavior and gene expression","volume":"175","author":"Jantzen","year":"2016","journal-title":"Aquat Toxicol"},{"key":"2025062603522293700_kfaf041-B26","doi-asserted-by":"publisher","first-page":"807632","DOI":"10.3389\/fphys.2022.807632","article-title":"Pancreas-its functions, disorders, and physiological impact on the mammals\u2019 organism","volume":"13","author":"Karpi\u0144ska","year":"2022","journal-title":"Front Physiol"},{"key":"2025062603522293700_kfaf041-B27","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1002\/aja.1002030302","article-title":"Stages of embryonic development of the zebrafish","volume":"203","author":"Kimmel","year":"1995","journal-title":"Dev Dyn"},{"key":"2025062603522293700_kfaf041-B29","doi-asserted-by":"crossref","first-page":"F106","DOI":"10.1136\/fn.88.2.F106","article-title":"Faecal elastase 1 levels in premature and full term infants","volume":"88","author":"Kori","year":"2003","journal-title":"Arch Dis Child Fetal Neonatal Ed"},{"key":"2025062603522293700_kfaf041-B30","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1542\/peds.66.4.556","article-title":"Development of functional response in human exocrine pancreas","volume":"66","author":"Lebenthal","year":"1980","journal-title":"Pediatrics"},{"key":"2025062603522293700_kfaf041-B31","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.placenta.2016.01.010","article-title":"In utero perfluorooctane sulfonate exposure causes low body weights of fetal rats: a mechanism study","volume":"39","author":"Li","year":"2016","journal-title":"Placenta"},{"key":"2025062603522293700_kfaf041-B32","doi-asserted-by":"publisher","first-page":"702","DOI":"10.2337\/dc08-1816","article-title":"Association among serum perfluoroalkyl chemicals, glucose homeostasis, and metabolic syndrome in adolescents and adults","volume":"32","author":"Lin","year":"2009","journal-title":"Diabetes Care"},{"key":"2025062603522293700_kfaf041-B33","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative pcr and the 2(-Delta Delta c(t)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"2025062603522293700_kfaf041-B34","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1016\/j.envint.2019.01.010","article-title":"Concentrations of perfluoroalkyl substances (PFASs) in human embryonic and fetal organs from first, second, and third trimester pregnancies","volume":"124","author":"Mamsen","year":"2019","journal-title":"Environ Int"},{"key":"2025062603522293700_kfaf041-B35","doi-asserted-by":"publisher","first-page":"106923","DOI":"10.1016\/j.aquatox.2024.106923","article-title":"Maternal PFOS exposure affects offspring development in NRF2-dependent and independent ways in zebrafish (Danio rerio)","volume":"271","author":"Marin","year":"2024","journal-title":"Aquat Toxicol"},{"key":"2025062603522293700_kfaf041-B36","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1136\/adc.68.1_Spec_No.62","article-title":"Ontogeny of human pancreatic exocrine function","volume":"68","author":"McClean","year":"1993","journal-title":"Arch Dis Child"},{"key":"2025062603522293700_kfaf041-B38","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1111\/j.1742-7843.2007.00188.x","article-title":"Developmental origins of adult health and disease: the role of periconceptional and foetal nutrition","volume":"102","author":"McMillen","year":"2008","journal-title":"Basic Clin Pharmacol Toxicol"},{"key":"2025062603522293700_kfaf041-B39","doi-asserted-by":"publisher","first-page":"909648","DOI":"10.3389\/fped.2022.909648","article-title":"Development of the human pancreas and its exocrine function","volume":"10","author":"Mehta","year":"2022","journal-title":"Front Pediatr"},{"key":"2025062603522293700_kfaf041-B40","doi-asserted-by":"publisher","first-page":"R867","DOI":"10.1152\/ajpregu.00072.2018","article-title":"Fetal undernutrition, placental insufficiency, and pancreatic beta-cell development programming in utero","volume":"315","author":"Mohan","year":"2018","journal-title":"Am J Physiol Regul Integr Comp Physiol"},{"key":"2025062603522293700_kfaf041-B41","doi-asserted-by":"publisher","first-page":"780","DOI":"10.1002\/etc4899","article-title":"Perfluorooctanesulfonic acid-induced toxicity on zebrafish embryos in the presence or absence of the chorion","volume":"40","author":"Mylroie","year":"2021","journal-title":"Environ Toxicol Chem"},{"key":"2025062603522293700_kfaf041-B42","doi-asserted-by":"publisher","first-page":"397","DOI":"10.5551\/jat.RV17039","article-title":"Adult-onset diseases in low birth weight infants: association with adipose tissue maldevelopment","volume":"27","author":"Nakano","year":"2020","journal-title":"J Atheroscler Thromb"},{"key":"2025062603522293700_kfaf041-B43","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1021\/es1019614","article-title":"Partition of environmental chemicals between maternal and fetal blood tissues","volume":"45","author":"Needham","year":"2011","journal-title":"Environ Sci Technol"},{"key":"2025062603522293700_kfaf041-B44","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1038\/s41387-018-0062-9","article-title":"Glucose homeostasis dependency on acini-islet-acinar (AIA) axis communication: a new possible pathophysiological hypothesis regarding diabetes mellitus","volume":"8","author":"Pierzynowski","year":"2018","journal-title":"Nutr Diabetes"},{"key":"2025062603522293700_kfaf041-B45","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1007\/s11892-020-01304-0","article-title":"Exocrine pancreatic insufficiency in type 1 and type 2 diabetes","volume":"20","author":"Radlinger","year":"2020","journal-title":"Curr Diab Rep"},{"key":"2025062603522293700_kfaf041-B46","doi-asserted-by":"publisher","first-page":"1425537","DOI":"10.3389\/ftox.2024.1425537","article-title":"Diverse PFAS produce unique transcriptomic changes linked to developmental toxicity in zebrafish","volume":"6","author":"Rericha","year":"2024","journal-title":"Front Toxicol"},{"key":"2025062603522293700_kfaf041-B47","doi-asserted-by":"publisher","first-page":"117743","DOI":"10.1016\/j.envres.2023.117743","article-title":"Estimation of per- and polyfluoroalkyl substances (PFAS) half-lives in human studies: a systematic review and meta-analysis","volume":"242","author":"Rosato","year":"2024","journal-title":"Environ Res"},{"key":"2025062603522293700_kfaf041-B48","doi-asserted-by":"publisher","first-page":"116644","DOI":"10.1016\/j.envpol.2021.116644","article-title":"Developmental exposures to perfluorooctanesulfonic acid (PFOS) impact embryonic nutrition, pancreatic morphology, and adiposity in the zebrafish, Danio rerio","volume":"275","author":"Sant","year":"2021","journal-title":"Environ Pollut"},{"key":"2025062603522293700_kfaf041-B49","doi-asserted-by":"publisher","first-page":"807","DOI":"10.1016\/j.envpol.2016.10.057","article-title":"Embryonic exposures to perfluorooctanesulfonic acid (PFOS) disrupt pancreatic organogenesis in the zebrafish, Danio rerio","volume":"220","author":"Sant","year":"2017","journal-title":"Environ Pollut"},{"key":"2025062603522293700_kfaf041-B50","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1007\/s40572-018-0183-2","article-title":"Zebrafish as a model for toxicological perturbation of yolk and nutrition in the early embryo","volume":"5","author":"Sant","year":"2018","journal-title":"Curr Environ Health Rep"},{"key":"2025062603522293700_kfaf041-B51","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat Methods"},{"key":"2025062603522293700_kfaf041-B52","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1242\/dmm.026633","article-title":"ptf1a(+), ela3l(-) cells are developmentally maintained progenitors for exocrine regeneration following extreme loss of acinar cells in zebrafish larvae","volume":"10","author":"Schmitner","year":"2017","journal-title":"Dis Model Mech"},{"key":"2025062603522293700_kfaf041-B53","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.envint.2015.11.016","article-title":"Serum perfluorinated chemicals, glucose homeostasis and the risk of diabetes in working-aged Taiwanese adults","volume":"88","author":"Su","year":"2016","journal-title":"Environ Int"},{"key":"2025062603522293700_kfaf041-B54","doi-asserted-by":"publisher","first-page":"3028","DOI":"10.1016\/j.devcel.2023.11.001","article-title":"Single-cell analysis of shared signatures and transcriptional diversity during zebrafish development","volume":"58","author":"Sur","year":"2023","journal-title":"Dev Cell"},{"key":"2025062603522293700_kfaf041-B55","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1038\/nprot.2007.514","article-title":"High-resolution in situ hybridization to whole-mount zebrafish embryos","volume":"3","author":"Thisse","year":"2008","journal-title":"Nat Protoc"},{"key":"2025062603522293700_kfaf041-B56","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.mce.2009.04.018","article-title":"Zebrafish pancreas development","volume":"312","author":"Tiso","year":"2009","journal-title":"Mol Cell Endocrinol"},{"key":"2025062603522293700_kfaf041-B57","doi-asserted-by":"publisher","first-page":"2495","DOI":"10.1210\/clinem\/dgab267","article-title":"Life-course exposure to perfluoroalkyl substances in relation to markers of glucose homeostasis in early adulthood","volume":"106","author":"Valvi","year":"2021","journal-title":"J Clin Endocrinol Metab"},{"key":"2025062603522293700_kfaf041-B58","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1002\/etc2403","article-title":"Investigating alternatives to the fish early-life stage test: a strategy for discovering and annotating adverse outcome pathways for early fish development","volume":"33","author":"Villeneuve","year":"2014","journal-title":"Environ Toxicol Chem"},{"key":"2025062603522293700_kfaf041-B59","doi-asserted-by":"publisher","first-page":"3898","DOI":"10.1021\/acs.est.8b07188","article-title":"Toxicokinetics of perfluorinated alkyl acids influences their toxic potency in the zebrafish embryo (Danio rerio)","volume":"53","author":"Vogs","year":"2019","journal-title":"Environ Sci Technol"},{"key":"2025062603522293700_kfaf041-B60","volume-title":"The zebrafish book. A guide for the laboratory use of zebrafish (danio rerio)","author":"Westerfield","year":"2000","edition":"4th ed"},{"key":"2025062603522293700_kfaf041-B61","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.envint.2017.06.007","article-title":"Per- and polyfluoroalkyl substances in human serum and urine samples from a residentially exposed community","volume":"106","author":"Worley","year":"2017","journal-title":"Environ Int"},{"key":"2025062603522293700_kfaf041-B62","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1016\/j.eehl.2024.04.007","article-title":"Early-life exposure to per- and polyfluoroalkyl substances: analysis of levels, health risk and binding abilities to transport proteins","volume":"3","author":"Xu","year":"2024","journal-title":"Eco Environ Health"},{"key":"2025062603522293700_kfaf041-B63","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1203\/00006450-197212000-00005","article-title":"Exocrine pancreas function in premature and full term neonates","volume":"6","author":"Zoppi","year":"1972","journal-title":"Pediatr Res"}],"container-title":["Toxicological Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/toxsci\/advance-article-pdf\/doi\/10.1093\/toxsci\/kfaf041\/62801906\/kfaf041.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/toxsci\/article-pdf\/206\/1\/46\/62801906\/kfaf041.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/toxsci\/article-pdf\/206\/1\/46\/62801906\/kfaf041.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T07:52:34Z","timestamp":1750924354000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/toxsci\/article\/206\/1\/46\/8099455"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,28]]},"references-count":59,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,3,28]]},"published-print":{"date-parts":[[2025,7,1]]}},"URL":"https:\/\/doi.org\/10.1093\/toxsci\/kfaf041","relation":{},"ISSN":["1096-6080","1096-0929"],"issn-type":[{"value":"1096-6080","type":"print"},{"value":"1096-0929","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,7]]},"published":{"date-parts":[[2025,3,28]]}}}