{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:06:52Z","timestamp":1788912412962,"version":"build-2803163510"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":3,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005188","name":"Tobacco-Related Disease Research Program","doi-asserted-by":"publisher","award":["TRDRP T321R5150"],"award-info":[{"award-number":["TRDRP T321R5150"]}],"id":[{"id":"10.13039\/100005188","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of California and UC Irvine Chao Family Comprehensive Cancer Center","award":["P30CA062203"],"award-info":[{"award-number":["P30CA062203"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tobacco and nicotine use remain leading preventable drivers of cancer risk, and both combustible cigarettes and electronic nicotine devices perturb immune function and hematopoiesis. However, side-by-side comparisons of their effects across the hematopoietic hierarchy remain limited. Here, we evaluate the impact of cigarette smoke extract (CSE) and e-cigarette vapor extract (EVE) on inflammatory responses in mature myeloid cells and on hematopoietic stem and progenitor cell function using complementary in vitro and in vivo approaches. Using a standardized protocol applied across multiple macrophage models, including RAW264.7 cells, THP-1 macrophages, primary mouse bone marrow-derived macrophages, and human peripheral blood mononuclear cells, we found that CSE consistently suppressed LPS-induced TNF-\u03b1 secretion in a dose-dependent manner, whereas EVE alone had minimal effect. CSE also induced foam cell formation in macrophages through a reactive oxygen species-dependent mechanism. Additionally, both CSE and EVE suppressed hematopoietic progenitor colony formation. To model real-world exposure patterns, we used a nose-only inhalation system to deliver combustible cigarette smoke followed by e-cigarette aerosol to mice. Sequential smoke and e-cigarette exposure increased platelet counts and myeloid cell frequency, effects not observed with smoking cessation alone. Myeloid skewing persisted through secondary transplantation, indicating durable, cell-intrinsic changes to the hematopoietic stem cell compartment. Together, these findings demonstrate that tobacco and e-cigarette exposures blunt innate immune responsiveness while driving lasting myeloid-biased hematopoiesis, with implications for infection susceptibility, cardiovascular disease, and hematologic malignancy risk.<\/jats:p>","DOI":"10.1093\/toxsci\/kfag096","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T08:15:11Z","timestamp":1788509711000},"source":"Crossref","is-referenced-by-count":0,"title":["Cigarette smoke and e-cigarette aerosol extracts induce myelopoiesis and suppress inflammatory cytokine production"],"prefix":"10.1093","volume":"209","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9242-7858","authenticated-orcid":false,"given":"Jeanette Y C","family":"Sullivan","sequence":"first","affiliation":[{"name":"Department of Biological Chemistry, School of Medicine, University of California, Irvine , Irvine, CA 92617,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen X","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, School of Medicine, University of California, Irvine , Irvine, CA 92617,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhong C","family":"Heidmann","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, School of Medicine, University of California, Irvine , Irvine, CA 92617,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Diaz","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, School of Medicine, University of California, Irvine , Irvine, CA 92617,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Brzechffa","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, School of Medicine, University of California, Irvine , Irvine, CA 92617,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane H","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, School of Medicine, University of California, Irvine , Irvine, CA 92617,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eli M","family":"Soyfer","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, School of Medicine, University of California, Irvine , Irvine, CA 92617,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0504-388X","authenticated-orcid":false,"given":"Nneamaka","family":"Iwobi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, School of Pharmacy, University of California, Irvine , Irvine, CA 92617,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Hasen","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health, College of Health Sciences, University of California, Irvine , Irvine, CA 92617,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Ting","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health, College of Health Sciences, University of California, Irvine , Irvine, CA 92617,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6176-7895","authenticated-orcid":false,"given":"Nicole","family":"Sparks","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health, College of Health Sciences, University of California, Irvine , Irvine, CA 92617,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael T","family":"Kleinman","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health, College of Health Sciences, University of California, Irvine , Irvine, CA 92617,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3701-6079","authenticated-orcid":false,"given":"Angela G","family":"Fleischman","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, School of Medicine, University of California, Irvine , Irvine, CA 92617,","place":["United States"]},{"name":"Division of Hematology\/Oncology, School of Medicine, University of California, Irvine , Irvine, CA 92617,","place":["United States"]},{"name":"Chao Family Comprehensive Cancer Center, University of California, Irvine , Irvine, CA 92617,","place":["United 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