{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:40:41Z","timestamp":1791981641769,"version":"4.4.0"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,12]]},"DOI":"10.1093\/toxsci\/kfn194","type":"journal-article","created":{"date-parts":[[2008,9,13]],"date-time":"2008-09-13T01:07:31Z","timestamp":1221268051000},"page":"464-478","source":"Crossref","is-referenced-by-count":210,"title":["LC-MS\u2013Based Characterization of the Peptide Reactivity of Chemicals to Improve the In Vitro Prediction of the Skin Sensitization Potential"],"prefix":"10.1093","volume":"106","author":[{"given":"Andreas","family":"Natsch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Gfeller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2008,9,12]]},"reference":[{"key":"key\n\t\t\t\t20170508170306_bib1","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1159\/000068288","article-title":"Reactivity of contact allergenic haptens to amino acid residues in a model carrier peptide, and characterization of formed peptide-hapten adducts","volume":"16","year":"2003","journal-title":"Skin Pharmacol. Appl. Skin Physiol."},{"key":"key\n\t\t\t\t20170508170306_bib2","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1021\/tx049935+","article-title":"Effect of glutathione on the covalent binding of the (13)C-labeled skin sensitizer 5-chloro-2-methylisothiazol-3-one to human serum albumin: identification of adducts by nuclear magnetic resonance, matrix-assisted laser desorption\/ionization mass spectrometry, and nanoelectrospray tandem mass spectrometry","volume":"17","year":"2004","journal-title":"Chemical Res. Toxicol."},{"key":"key\n\t\t\t\t20170508170306_bib3","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/S0278-6915(01)00130-2","article-title":"Local lymph node assay\u2014Validation and use in practice","volume":"40","year":"2002","journal-title":"Food Chem. Toxicol."},{"key":"key\n\t\t\t\t20170508170306_bib4","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1021\/tx050312l","article-title":"Cytosolic and nuclear protein targets of thiol-reactive electrophiles","volume":"19","year":"2006","journal-title":"Chemical Res. Toxicol."},{"key":"key\n\t\t\t\t20170508170306_bib5","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1111\/j.0105-1873.2005.00683.x","article-title":"Hapten-protein binding: from theory to practical application in the in vitro prediction of skin sensitization","volume":"53","year":"2005","journal-title":"Contact Dermatitis"},{"key":"key\n\t\t\t\t20170508170306_bib6","first-page":"215","article-title":"Chemical reactivity measurement and the predictive identification of skin sensitisers: The report and recommendations of ECVAM workshop 64a","volume":"36","year":"2008","journal-title":"ATLA"},{"key":"key\n\t\t\t\t20170508170306_bib7","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1111\/j.0105-1873.2004.00290.x","article-title":"A chemical dataset for evaluation of alternative approaches to skin-sensitization testing","volume":"50","year":"2004","journal-title":"Contact Dermatitis"},{"key":"key\n\t\t\t\t20170508170306_bib8","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1093\/toxsci\/kfh213","article-title":"Development of a peptide reactivity assay for screening contact allergens","volume":"81","year":"2004","journal-title":"Toxicol. Sci."},{"key":"key\n\t\t\t\t20170508170306_bib9","first-page":"414","article-title":"Quantification of chemical peptide reactivity for screening contact allergens: A classification tree model approach","volume":"97","year":"2007","journal-title":"Toxicol. Sci."},{"key":"key\n\t\t\t\t20170508170306_bib10","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1038\/sj.jid.5700319","article-title":"Identification of gene expression changes induced by chemical allergens in dendritic cells: Opportunities for skin sensitization testing","volume":"126","year":"2006","journal-title":"J. Invest. Dermatol."},{"key":"key\n\t\t\t\t20170508170306_bib11","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.1016\/S0278-6915(03)00223-0","article-title":"Classification of contact allergens according to potency: Proposals","volume":"41","year":"2003","journal-title":"Food Chem. Toxicol."},{"key":"key\n\t\t\t\t20170508170306_bib12","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1093\/toxsci\/kfm259","article-title":"Skin sensitizers induce antioxidant response element dependent genes: Application to the in vitro testing of the sensitization potential of chemicals","volume":"102","year":"2008","journal-title":"Toxicol. Sci."},{"key":"key\n\t\t\t\t20170508170306_bib13","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1016\/j.tiv.2007.03.016","article-title":"Use and limitations of a peptide reactivity assay to predict fragrance allergens in vitro","volume":"21","year":"2007","journal-title":"Toxicol. In Vitro"},{"key":"key\n\t\t\t\t20170508170306_bib14","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1021\/tx049758c","article-title":"A conjugated diene identified as a prohapten: Contact allergenic activity and chemical reactivity of proposed epoxide metabolites","volume":"18","year":"2005","journal-title":"Chem. Res. Toxicol."},{"key":"key\n\t\t\t\t20170508170306_bib15","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1021\/tx060102o","article-title":"Mechanistic applicability domain for non-animal based prediction of toxicological endpoints. QSAR analysis of the Schiff base applicability domain for skin sensitization","volume":"19","year":"2006","journal-title":"Chem. Res. Toxicol."},{"key":"key\n\t\t\t\t20170508170306_bib16","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1002\/jat.1293","article-title":"Chemical reactivity indices and mechanism-based read-across for non-animal based assessment of skin sensitization potential","volume":"28","year":"2008","journal-title":"J. Appl. Toxicol."},{"key":"key\n\t\t\t\t20170508170306_bib17","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1021\/tx700024w","article-title":"Mechanistic applicability domain classification of a local lymph node assay dataset for skin sensitization","volume":"20","year":"2007","journal-title":"Chem. Res. Toxicol."},{"key":"key\n\t\t\t\t20170508170306_bib18","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1016\/j.tiv.2005.10.014","article-title":"Development of an in vitro skin sensitization test using human cell lines: Human cell line activation test (h-CLAT) II. An inter-laboratory study of the h-CLAT","volume":"20","year":"2006","journal-title":"Toxicol. In Vitro"},{"key":"key\n\t\t\t\t20170508170306_bib19","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1080\/10659360500204152","article-title":"Structure-activity relationships for reactivity of carbonyl-containing compounds with glutathione","volume":"16","year":"2005","journal-title":"SAR QSAR Environ. Res."},{"key":"key\n\t\t\t\t20170508170306_bib20","volume-title":"Allergic Contact Dermatitis: Chemical and Metabolic Mechanisms","year":"2001"},{"key":"key\n\t\t\t\t20170508170306_bib21","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1021\/tx050303c","article-title":"A semiquantitative method for the determination of reactive metabolite conjugate levels in vitro utilizing liquid chromatography-tandem mass spectrometry and novel quaternary ammonium glutathione analogues","volume":"19","year":"2006","journal-title":"Chem. Res. Toxicol."},{"key":"key\n\t\t\t\t20170508170306_bib22","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1021\/tx600277y","article-title":"Screening and identification of GSH-trapped reactive metabolites using hybrid triple quadruple linear ion trap mass spectrometry","volume":"20","year":"2007","journal-title":"Chem. Res. Toxicol."}],"container-title":["Toxicological Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/toxsci\/article-pdf\/106\/2\/464\/16679584\/kfn194.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,8,24]],"date-time":"2017-08-24T02:19:29Z","timestamp":1503541169000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/toxsci\/article-lookup\/doi\/10.1093\/toxsci\/kfn194"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,9,12]]},"references-count":22,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2008,9,12]]},"published-print":{"date-parts":[[2008,12]]}},"URL":"https:\/\/doi.org\/10.1093\/toxsci\/kfn194","relation":{},"ISSN":["1096-6080","1096-0929"],"issn-type":[{"value":"1096-6080","type":"print"},{"value":"1096-0929","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,9,12]]}}}