{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T16:29:55Z","timestamp":1781886595870,"version":"3.54.5"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T00:00:00Z","timestamp":1583107200000},"content-version":"vor","delay-in-days":366,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K08 HL105537"],"award-info":[{"award-number":["K08 HL105537"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 ES027574"],"award-info":[{"award-number":["R01 ES027574"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000139","name":"United States Environmental Protection Agency","doi-asserted-by":"publisher","award":["R-82811201"],"award-info":[{"award-number":["R-82811201"]}],"id":[{"id":"10.13039\/100000139","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Sex differences clearly exist in incidence, susceptibility, and severity of airway disease and in pulmonary responses to air pollutants such as ozone (O3). Prior rodent O3 exposure studies demonstrate sex-related differences in the expression of lung inflammatory mediators and signaling. However, whether or not sex modifies O3-induced airway physiologic responses remains less explored. To address this, we exposed 8- to 10-week-old male and female C57BL\/6 mice to either 1 or 2\u2009ppm O3 or filtered air (FA) for 3\u2009h. At 12, 24, 48, and 72\u2009h following exposure, we assessed airway hyperresponsiveness to methacholine (MCh), bronchoalveolar lavage fluid cellularity, cytokines and total protein\/albumin, serum progesterone, and whole lung immune cells by flow cytometry. Male mice generated consistent airway hyperresponsiveness to MCh at all time points following exposure. Alternatively, females had less consistent airway physiologic responses to MCh, which were more variable between individual experiments and did not correlate with serum progesterone levels. Bronchoalveolar lavage fluid total cells peaked at 12\u2009h and were persistently elevated through 72\u2009h. At 48\u2009h, bronchoalveolar lavage cells were greater in females versus males. Bronchoalveolar lavage fluid cytokines and total protein\/albumin increased following O3 exposure without sex differences. Flow cytometry of whole lung tissue identified dynamic O3-induced immune cell changes also independent of sex. Our results indicate sex differences in acute O3-induced airway physiology responses and airspace influx without significant difference in other injury and inflammation measures. This study highlights the importance of considering sex as a biological variable in acute O3-induced airway physiology responses.<\/jats:p>","DOI":"10.1093\/toxsci\/kfz056","type":"journal-article","created":{"date-parts":[[2019,2,27]],"date-time":"2019-02-27T01:44:34Z","timestamp":1551231874000},"page":"499-510","source":"Crossref","is-referenced-by-count":55,"title":["Sex Modifies Acute Ozone-Mediated Airway Physiologic Responses"],"prefix":"10.1093","volume":"169","author":[{"given":"Anastasiya","family":"Birukova","sequence":"first","affiliation":[{"name":"Department of Medicine, Duke University, Durham, North Carolina 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Cyphert-Daly","sequence":"first","affiliation":[{"name":"Department of Medicine, Duke University, Durham, North Carolina 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert Ian","family":"Cumming","sequence":"first","affiliation":[{"name":"Department of Medicine, Duke University, Durham, North Carolina 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yen-Rei","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Medicine, Duke University, Durham, North Carolina 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kymberly M","family":"Gowdy","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, East Carolina University, Greenville, North Carolina 27858"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loretta G","family":"Que","sequence":"first","affiliation":[{"name":"Department of Medicine, Duke University, Durham, North Carolina 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M","family":"Tighe","sequence":"first","affiliation":[{"name":"Department of Medicine, Duke University, Durham, North Carolina 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,3,2]]},"reference":[{"key":"2020030521271146700_kfz056-B1","doi-asserted-by":"crossref","first-page":"L118","DOI":"10.1152\/ajplung.00080.2013","article-title":"Endogenous osteopontin promotes ozone-induced neutrophil recruitment to the lungs and airway hyperresponsiveness to methacholine","volume":"305","author":"Barreno","year":"2013","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol"},{"key":"2020030521271146700_kfz056-B2","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1152\/japplphysiol.01274.2001","article-title":"Time dependence of recruitment and derecruitment in the lung: A theoretical model","volume":"93","author":"Bates","year":"2002","journal-title":"J. Appl. Physiol."},{"key":"2020030521271146700_kfz056-B3","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1152\/japplphysiol.00706.2002","article-title":"Measuring lung function in mice: The phenotyping uncertainty principle","volume":"94","author":"Bates","year":"2003","journal-title":"J. Appl. Physiol."},{"key":"2020030521271146700_kfz056-B4","doi-asserted-by":"crossref","first-page":"2372","DOI":"10.1001\/jama.292.19.2372","article-title":"Ozone and short-term mortality in 95 US urban communities, 1987\u20132000","volume":"292","author":"Bell","year":"2004","journal-title":"Jama"},{"key":"2020030521271146700_kfz056-B5","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1080\/08958370701665475","article-title":"Acute pulmonary function response to ozone in young adults as a function of body mass index","volume":"19","author":"Bennett","year":"2007","journal-title":"Inhal. Toxicol"},{"key":"2020030521271146700_kfz056-B6","doi-asserted-by":"crossref","first-page":"e0160030.","DOI":"10.1371\/journal.pone.0160030","article-title":"Effect of obesity on acute ozone-induced changes in airway function, reactivity, and inflammation in adult females","volume":"11","author":"Bennett","year":"2016","journal-title":"PLoS One"},{"key":"2020030521271146700_kfz056-B7","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1289\/ehp.1104851","article-title":"Health benefits from large-scale ozone reduction in the United States","volume":"120","author":"Berman","year":"2012","journal-title":"Environ. Health Perspect"},{"key":"2020030521271146700_kfz056-B8","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1164\/rccm.200203-245OC","article-title":"Clinical, radiologic, and induced sputum features of chronic obstructive pulmonary disease in nonsmokers: A descriptive study","volume":"166","author":"Birring","year":"2002","journal-title":"Am. J. Resp. Crit. Care Med"},{"key":"2020030521271146700_kfz056-B9","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1159\/000312635","article-title":"Airway inflammation and remodeling in two mouse models of asthma: Comparison of males and females","volume":"153","author":"Blacquiere","year":"2010","journal-title":"Int. Arch. Allergy Immunol"},{"key":"2020030521271146700_kfz056-B10","doi-asserted-by":"crossref","first-page":"L1150","DOI":"10.1152\/ajplung.00018.2015","article-title":"Sex differences in the expression of lung inflammatory mediators in response to ozone","volume":"309","author":"Cabello","year":"2015","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol"},{"key":"2020030521271146700_kfz056-B11","doi-asserted-by":"crossref","first-page":"621","DOI":"10.4049\/jimmunol.177.1.621","article-title":"Gender differences in murine airway responsiveness and lipopolysaccharide-induced inflammation","volume":"177","author":"Card","year":"2006","journal-title":"J. Immunol"},{"key":"2020030521271146700_kfz056-B12","doi-asserted-by":"crossref","first-page":"L908","DOI":"10.1152\/ajplung.00407.2006","article-title":"Male sex hormones promote vagally mediated reflex airway responsiveness to cholinergic stimulation","volume":"292","author":"Card","year":"2007","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol"},{"key":"2020030521271146700_kfz056-B13","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1164\/rccm.200509-1493OC","article-title":"Spontaneous airway hyperresponsiveness in estrogen receptor-alpha-deficient mice","volume":"175","author":"Carey","year":"2007","journal-title":"Am. J. Resp. Critical Care Med"},{"key":"2020030521271146700_kfz056-B15","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1165\/rcmb.2018-0099OC","article-title":"Sex differences in pulmonary responses to ozone in mice. Role of the microbiome","volume":"60","author":"Cho","year":"2019","journal-title":"Am. J. Resp. Cell Mol. Biol"},{"key":"2020030521271146700_kfz056-B16","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1165\/rcmb.2017-0404OC","article-title":"The microbiome regulates pulmonary responses to ozone in mice","volume":"59","author":"Cho","year":"2018","journal-title":"Am. J. Resp. Cell Mol. Biol"},{"key":"2020030521271146700_kfz056-B17","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.1016\/S0140-6736(17)30505-6","article-title":"Estimates and 25-year trends of the global burden of disease attributable to ambient air pollution: An analysis of data from the global burden of diseases study 2015","volume":"389","author":"Cohen","year":"2017","journal-title":"Lancet"},{"key":"2020030521271146700_kfz056-B18","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1165\/ajrcmb\/4.1.72","article-title":"Exposure of humans to ambient levels of ozone for 6.6 hours causes cellular and biochemical changes in the lung","volume":"4","author":"Devlin","year":"1991","journal-title":"Am. J. Resp. Cell Mol. Biol"},{"key":"2020030521271146700_kfz056-B19","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1152\/jappl.2000.89.5.1804","article-title":"Bronchial reactivity of healthy subjects: 18\u201320 h postexposure to ozone","volume":"89","author":"Foster","year":"2000","journal-title":"J. Appl. Physiol"},{"key":"2020030521271146700_kfz056-B20","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1093\/toxsci\/kfw226","article-title":"Editor's highlight: Ccr2 regulates inflammatory cell accumulation in the lung and tissue injury following ozone exposure","volume":"155","author":"Francis","year":"2017","journal-title":"Toxicol. Sci"},{"key":"2020030521271146700_kfz056-B21","doi-asserted-by":"crossref","first-page":"18.","DOI":"10.1186\/s13293-018-0177-7","article-title":"Sex-specific microRNA expression networks in an acute mouse model of ozone-induced lung inflammation","volume":"9","author":"Fuentes","year":"2018","journal-title":"Biol. Sex Differ"},{"key":"2020030521271146700_kfz056-B22","doi-asserted-by":"crossref","first-page":"52.","DOI":"10.1186\/1465-9921-7-52","article-title":"Female smokers beyond the perimenopausal period are at increased risk of chronic obstructive pulmonary disease: A systematic review and meta-analysis","volume":"7","author":"Gan","year":"2006","journal-title":"Resp. Res"},{"key":"2020030521271146700_kfz056-B23","doi-asserted-by":"crossref","first-page":"11309","DOI":"10.1074\/jbc.M802400200","article-title":"Hyaluronan mediates ozone-induced airway hyperresponsiveness in mice","volume":"284","author":"Garantziotis","year":"2009","journal-title":"J. Biol. Chem"},{"key":"2020030521271146700_kfz056-B24","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1152\/jappl.1992.73.2.427","article-title":"Mechanical impedances of lungs and chest wall in the cat","volume":"73","author":"Hantos","year":"1992","journal-title":"J. Appl. Physiol"},{"key":"2020030521271146700_kfz056-B25","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1152\/japplphysiol.00490.2003","article-title":"Distribution and reproducibility of spirometric response to ozone by gender and age","volume":"95","author":"Hazucha","year":"2003","journal-title":"J. Appl. Physiol"},{"key":"2020030521271146700_kfz056-B26","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1046\/j.1365-2222.2003.01743.x","article-title":"Progesterone increases airway eosinophilia and hyper-responsiveness in a murine model of allergic asthma","volume":"33","author":"Hellings","year":"2003","journal-title":"Clin. Exp. Allergy"},{"key":"2020030521271146700_kfz056-B27","first-page":"4.","article-title":"Measuring the lung function in the mouse: The challenge of size","volume":"4","author":"Irvin","year":"2003","journal-title":"Resp. Res"},{"key":"2020030521271146700_kfz056-B28","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1056\/NEJMoa0803894","article-title":"Long-term ozone exposure and mortality","volume":"360","author":"Jerrett","year":"2009","journal-title":"N. Eng. J. Med"},{"key":"2020030521271146700_kfz056-B29","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1152\/japplphysiol.00300.2006","article-title":"Pulmonary responses to acute ozone exposure in fasted mice: Effect of leptin administration","volume":"102","author":"Johnston","year":"2007","journal-title":"J. Appl. Physiol"},{"key":"2020030521271146700_kfz056-B30","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1038\/ng1297-475","article-title":"Linkage analysis of susceptibility to ozone-induced lung inflammation in inbred mice","volume":"17","author":"Kleeberger","year":"1997","journal-title":"Nat. Genet"},{"key":"2020030521271146700_kfz056-B31","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1038\/nri.2016.90","article-title":"Sex differences in immune responses","volume":"16","author":"Klein","year":"2016","journal-title":"Nat. Rev. Immunol"},{"key":"2020030521271146700_kfz056-B32","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1177\/0192623317728135","article-title":"Innate lymphoid cells mediate pulmonary eosinophilic inflammation, airway mucous cell metaplasia, and type 2 immunity in mice exposed to ozone","volume":"45","author":"Kumagai","year":"2017","journal-title":"Toxicol. Pathol"},{"key":"2020030521271146700_kfz056-B33","doi-asserted-by":"crossref","first-page":"2562","DOI":"10.4049\/jimmunol.180.4.2562","article-title":"Ccr2+ monocyte-derived dendritic cells and exudate macrophages produce influenza-induced pulmonary immune pathology and mortality","volume":"180","author":"Lin","year":"2008","journal-title":"J. Immunol"},{"key":"2020030521271146700_kfz056-B34","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1289\/ehp.1409495","article-title":"A time-stratified case-crossover study of ambient ozone exposure and emergency department visits for specific respiratory diagnoses in California (2005\u20132008)","volume":"124","author":"Malig","year":"2016","journal-title":"Environ. Health Perspect"},{"key":"2020030521271146700_kfz056-B35","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1289\/EHP272","article-title":"Il-33 drives augmented responses to ozone in obese mice","volume":"125","author":"Mathews","year":"2017","journal-title":"Environ. Health Perspect"},{"key":"2020030521271146700_kfz056-B36","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1165\/rcmb.2017-0071OC","article-title":"Augmented responses to ozone in obese mice require il-17a and gastrin-releasing peptide","volume":"58","author":"Mathews","year":"2018","journal-title":"Am. J. Resp. Cell Mol. Biol"},{"key":"2020030521271146700_kfz056-B37","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1080\/00039896.1991.9937441","article-title":"Respiratory response of humans exposed to low levels of ozone for 6.6 hours","volume":"46","author":"McDonnell","year":"1991","journal-title":"Arch. Environ. Health"},{"key":"2020030521271146700_kfz056-B38","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1007\/s11882-007-0012-4","article-title":"Are there reasons why adult asthma is more common in females?","volume":"7","author":"Melgert","year":"2007","journal-title":"Curr. Allergy Asthma Rep"},{"key":"2020030521271146700_kfz056-B39","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1165\/rcmb.2013-0086MA","article-title":"Flow cytometric analysis of macrophages and dendritic cell subsets in the mouse lung","volume":"49","author":"Misharin","year":"2013","journal-title":"Am. J. Resp. Cell Mol. Biol"},{"key":"2020030521271146700_kfz056-B40","doi-asserted-by":"crossref","first-page":"16.","DOI":"10.1186\/s13293-016-0069-7","article-title":"Sex-specific il-6-associated signaling activation in ozone-induced lung inflammation","volume":"7","author":"Mishra","year":"2016","journal-title":"Biol. Sex Differ"},{"key":"2020030521271146700_kfz056-B41","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1289\/ehp.7413","article-title":"Acute ozone-induced differential gene expression profiles in rat lung","volume":"113","author":"Nadadur","year":"2005","journal-title":"Environ. Health Perspect"},{"key":"2020030521271146700_kfz056-B42","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/ije\/27.1.27","article-title":"Mortality in women and men in relation to smoking","volume":"27","author":"Prescott","year":"1998","journal-title":"Int. J. Epidemiol"},{"key":"2020030521271146700_kfz056-B43","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1152\/japplphysiol.00337.2011","article-title":"Pulmonary function, bronchial reactivity, and epithelial permeability are response phenotypes to ozone and develop differentially in healthy humans","volume":"111","author":"Que","year":"2011","journal-title":"J. Appl. Physiol"},{"key":"2020030521271146700_kfz056-B44","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/S1081-1206(10)61533-5","article-title":"The relationship of sex to asthma prevalence, health care utilization, and medications in a large managed care organization","volume":"91","author":"Schatz","year":"2003","journal-title":"Ann. Allergy Asthma Immunol"},{"key":"2020030521271146700_kfz056-B45","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1002\/ppul.1950190304","article-title":"Prevalence of respiratory symptoms in Swiss children: Is bronchial asthma really more prevalent in boys?","volume":"19","author":"Sennhauser","year":"1995","journal-title":"Pediatr. Pulmonol"},{"key":"2020030521271146700_kfz056-B46","doi-asserted-by":"crossref","first-page":"25.","DOI":"10.1186\/s12940-015-0010-2","article-title":"Ambient ozone exposure and children's acute asthma in New York City: A case-crossover analysis","volume":"14","author":"Sheffield","year":"2015","journal-title":"Environ. Health"},{"key":"2020030521271146700_kfz056-B47","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1152\/japplphysiol.00381.2001","article-title":"Ozone-induced airway hyperresponsiveness is reduced in immature mice","volume":"92","author":"Shore","year":"2002","journal-title":"J. Appl. Physiol"},{"key":"2020030521271146700_kfz056-B48","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1164\/rccm.200908-1201OC","article-title":"Short-term associations between ambient air pollutants and pediatric asthma emergency department visits","volume":"182","author":"Strickland","year":"2010","journal-title":"Am. J. Resp. Critical Care Med"},{"key":"2020030521271146700_kfz056-B49","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.taap.2015.02.002","article-title":"Regulation of ozone-induced lung inflammation and injury by the beta-galactoside-binding lectin galectin-3","volume":"284","author":"Sunil","year":"2015","journal-title":"Toxicol. Appl. Pharmacol"},{"key":"2020030521271146700_kfz056-B50","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1093\/toxsci\/kft071","article-title":"Ozone-induced injury and oxidative stress in bronchiolar epithelium are associated with altered pulmonary mechanics","volume":"133","author":"Sunil","year":"2013","journal-title":"Toxicol. Sci"},{"key":"2020030521271146700_kfz056-B51","doi-asserted-by":"crossref","first-page":"24.","DOI":"10.1186\/1472-6874-11-24","article-title":"The role of female hormones on lung function in chronic lung diseases","volume":"11","author":"Tam","year":"2011","journal-title":"BMC Womens Health"},{"key":"2020030521271146700_kfz056-B52","doi-asserted-by":"crossref","DOI":"10.1165\/rcmb.2017-0357OC","article-title":"Euthanasia and lavage mediated effects on bronchoalveolar measures of lung injury and inflammation","author":"Tighe","year":"2018","journal-title":"Am. J. Resp. Cell Mol. Biol"},{"key":"2020030521271146700_kfz056-B53","doi-asserted-by":"crossref","first-page":"4800","DOI":"10.4049\/jimmunol.1101312","article-title":"Ozone inhalation promotes cx3cr1-dependent maturation of resident lung macrophages that limit oxidative stress and inflammation","volume":"187","author":"Tighe","year":"2011","journal-title":"J. Immunol"},{"key":"2020030521271146700_kfz056-B54","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1152\/japplphysiol.01129.2001","article-title":"Airway and tissue mechanics in a murine model of asthma: Alveolar capsule vs. Forced oscillations","volume":"93","author":"Tomioka","year":"2002","journal-title":"J. Appl. Physiol"},{"key":"2020030521271146700_kfz056-B55","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1210\/er.2010-0031","article-title":"Sex differences and sex steroids in lung health and disease","volume":"33","author":"Townsend","year":"2012","journal-title":"Endocr. Rev"},{"key":"2020030521271146700_kfz056-B56","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1289\/ehp.1408852","article-title":"Novel roles for notch3 and notch4 receptors in gene expression and susceptibility to ozone-induced lung inflammation in mice","volume":"123","author":"Verhein","year":"2015","journal-title":"Environ. Health Perspect"},{"key":"2020030521271146700_kfz056-B57","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1016\/j.jaci.2010.06.018","article-title":"Gender differences in asthma development and remission during transition through puberty: The tracking adolescents' individual lives survey (trails) study","volume":"126","author":"Vink","year":"2010","journal-title":"J. Allergy Clin. Immunol"},{"key":"2020030521271146700_kfz056-B58","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.envres.2014.02.013","article-title":"Association of short-term increases in ambient air pollution and timing of initial asthma diagnosis among medicaid-enrolled children in a metropolitan area","volume":"131","author":"Wendt","year":"2014","journal-title":"Environ. Res"},{"key":"2020030521271146700_kfz056-B59","doi-asserted-by":"crossref","first-page":"L1168","DOI":"10.1152\/ajplung.00393.2014","article-title":"Innate and ozone-induced airway hyperresponsiveness in obese mice: Role of TNF-alpha","volume":"308","author":"Williams","year":"2015","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol"},{"key":"2020030521271146700_kfz056-B60","doi-asserted-by":"crossref","first-page":"e0150606.","DOI":"10.1371\/journal.pone.0150606","article-title":"A protocol for the comprehensive flow cytometric analysis of immune cells in normal and inflamed murine non-lymphoid tissues","volume":"11","author":"Yu","year":"2016","journal-title":"PLoS One"},{"key":"2020030521271146700_kfz056-B61","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1165\/rcmb.2012-0366MA","article-title":"Identification of myeloid cell subsets in murine lungs using flow cytometry","volume":"49","author":"Zaynagetdinov","year":"2013","journal-title":"Am. J. Resp. Cell Mol. Biol"}],"container-title":["Toxicological Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/toxsci\/article-pdf\/169\/2\/499\/28745405\/kfz056.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/toxsci\/article-pdf\/169\/2\/499\/28745405\/kfz056.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,6]],"date-time":"2020-03-06T02:34:27Z","timestamp":1583462067000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/toxsci\/article\/169\/2\/499\/5368496"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,2]]},"references-count":60,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,3,2]]},"published-print":{"date-parts":[[2019,6,1]]}},"URL":"https:\/\/doi.org\/10.1093\/toxsci\/kfz056","relation":{},"ISSN":["1096-6080","1096-0929"],"issn-type":[{"value":"1096-6080","type":"print"},{"value":"1096-0929","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,6]]},"published":{"date-parts":[[2019,3,2]]}}}