{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:09:04Z","timestamp":1792786144551,"version":"4.5.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Australian Research Council project","award":["DP210102178"],"award-info":[{"award-number":["DP210102178"]}],"id":[{"id":"https:\/\/ror.org\/05mmh0f86","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Quantifying ascorbate (ASC) and glutathione (GSH) in woody perennial tissues presents significant methodological challenges due to complex tissue matrices and the lability of these redox-active metabolites. We adapted established enzymatic microplate assays for ASC and GSH quantification to mature dormant grapevine buds, a structure containing protective scales, hairs and lignified components. Key optimizations included: (i) use of 1\u00a0M HClO\u2084 rather than 0.2\u00a0M HCl for effective enzyme inactivation and matrix disruption; (ii) sample-to-solvent ratios of 1:10 to 1:15; and (iii) extended centrifugation to effectively sediment organic components. Standard addition experiments revealed significant matrix effects, with recovery of ~80% for ASC and 65% for GSH. The optimized method successfully determined &amp;gt;90% reduction status for both metabolites in mature buds. Critically, we observed severe ASC instability during frozen storage, with ~80% loss of ASC after 4 months at \u221280\u00a0\u00b0C, while GSH remained stable. These findings underscore the necessity of immediate analysis for ASC quantification in woody tissues and provide a validated protocol for studying redox metabolism in perennial bud tissues.<\/jats:p>","DOI":"10.1093\/treephys\/tpag103","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T11:57:37Z","timestamp":1784807857000},"source":"Crossref","is-referenced-by-count":0,"title":["Optimization of ascorbate and glutathione assays for woody perennial bud tissues: overcoming matrix effects and storage instability"],"prefix":"10.1093","volume":"46","author":[{"given":"Juwita R","family":"Dewi","sequence":"first","affiliation":[{"name":"Department of Environmental Science, Graduate School, Universitas Brawijaya , Jl. MT. Haryono No. 169, Ketawanggede, Lowokwaru, Malang , East Java, 65145,","place":["Indonesia"]},{"name":"The UWA Institute of Agriculture, The University of Western Australia , 35 Stirling Hwy, Perth, 6009 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pragya","family":"Poudel","sequence":"additional","affiliation":[{"name":"The UWA Institute of Agriculture, The University of Western Australia , 35 Stirling Hwy, Perth, 6009 ,","place":["Australia"]},{"name":"The School of Molecular Sciences, The University of Western Australia , 35 Stirling Hwy, Perth, 6009 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyi","family":"Xu","sequence":"additional","affiliation":[{"name":"The UWA Institute of Agriculture, The University of Western Australia , 35 Stirling Hwy, Perth, 6009 ,","place":["Australia"]},{"name":"The School of Molecular Sciences, The University of Western Australia , 35 Stirling Hwy, Perth, 6009 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1854-3164","authenticated-orcid":false,"given":"Santiago","family":"Signorelli","sequence":"additional","affiliation":[{"name":"Food and Plant Biology group, Department of Plant Biology, School of Agriculture, Universidad de la Rep\u00fablica , 780 Av. 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