{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T10:05:42Z","timestamp":1746093942639,"version":"3.37.3"},"reference-count":7,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2020,7,25]],"date-time":"2020-07-25T00:00:00Z","timestamp":1595635200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Universidad Nacional de San Antonio Abad del Cusco, Cusco, Peru","award":["R-697-2012-UNSAAC"],"award-info":[{"award-number":["R-697-2012-UNSAAC"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,10,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Cutaneous leishmaniasis (CL) is generally diagnosed by molecular methods, including PCR, using biopsy samples, skin scrapings and clinical exudates. In this study, we assessed the PCR performance for diagnosis of CL using skin of biopsy samples vs PCR of skin lesion exudate samples on filter paper and compared the diagnostic concordance of PCR using both sampling methods.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We assessed the PCR performance using 80 skin biopsy samples and 80 filter paper samples containing exudates from skin lesions obtained from 74 patients with clinical suspicion of CL in Cusco, Peru.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>: PCR using skin biopsy samples had superior diagnostic accuracy compared with filter paper PCR (62.5% [50\/80] vs 38.7% [31\/80], respectively; p\u02c20.005) and the diagnostic concordance between both sampling methods was \u2018moderate\u2019 (kappa coefficient=0.50, 95% CI 0.98 to 1.0).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>PCR using biopsy samples remains the standard for diagnosis of CL.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/trstmh\/traa052","type":"journal-article","created":{"date-parts":[[2020,7,2]],"date-time":"2020-07-02T19:13:40Z","timestamp":1593717220000},"page":"721-724","source":"Crossref","is-referenced-by-count":3,"title":["PCR performance for the diagnosis of cutaneous leishmaniasis caused by Leishmania viannia complex using biopsy samples, compared with exudate samples from skin lesions on filter paper"],"prefix":"10.1093","volume":"114","author":[{"given":"Yahanda Gisela","family":"Apaza-Castillo","sequence":"first","affiliation":[{"name":"Facultad de Ciencias, Universidad Nacional de San Antonio Abad del Cusco, Cusco 08001, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8942-8868","authenticated-orcid":false,"given":"Elsa Gladys","family":"Aguilar-Ancori","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias, Universidad Nacional de San Antonio Abad del Cusco, Cusco 08001, Peru"},{"name":"Instituto Universitario de Enfermedades Tropicales y Biomedicina de Cusco \u2013 UNSAAC, Cusco 08001, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mercedes Maritza","family":"Quispe-Fl\u00f3rez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias, Universidad Nacional de San Antonio Abad del Cusco, Cusco 08001, Peru"},{"name":"Instituto Universitario de Enfermedades Tropicales y Biomedicina de Cusco \u2013 UNSAAC, Cusco 08001, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0471-6746","authenticated-orcid":false,"given":"Max Carlos","family":"Ram\u00edrez-Soto","sequence":"additional","affiliation":[{"name":"School of Public Health and Administration, Universidad Peruana Cayetano Heredia, Lima 15102, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa Luz","family":"Pacheco-Venero","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias, Universidad Nacional de San Antonio Abad del Cusco, Cusco 08001, Peru"},{"name":"Instituto Universitario de Enfermedades Tropicales y Biomedicina de Cusco \u2013 UNSAAC, Cusco 08001, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,7,25]]},"reference":[{"issue":"5","key":"2020100421241358400_bib1","doi-asserted-by":"crossref","first-page":"895","DOI":"10.4269\/ajtmh.13-0582","article-title":"Comparison of clinical samples and methods in chronic cutaneous leishmaniasis","volume":"91","author":"Eroglu","year":"2014","journal-title":"Am J Trop Med Hyg"},{"issue":"2","key":"2020100421241358400_bib2","doi-asserted-by":"crossref","first-page":"ofx037","DOI":"10.1093\/ofid\/ofx037","article-title":"Sensitive molecular diagnostics for cutaneous leishmaniasis","volume":"4","author":"Sagi","year":"2017","journal-title":"Open Forum Infect Dis"},{"key":"2020100421241358400_bib3","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1086\/648730","article-title":"Detection and species identification of Leishmania kDNA from filter-paper lesion impressions for patients with American cutaneous leishmaniasis","volume":"50","author":"Boggild","year":"2010","journal-title":"Clin Infect Dis"},{"issue":"3","key":"2020100421241358400_bib4","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1128\/JCM.02457-10","article-title":"Diagnostic performance of filter paper lesion impression PCR for secondarily infected ulcers and nonulcerative lesions caused by cutaneous leishmaniasis","volume":"49","author":"Boggild","year":"2011","journal-title":"J Clin Microbiol"},{"key":"2020100421241358400_bib5","volume-title":"Molecular Cloning. 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