{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T12:17:14Z","timestamp":1784809034514,"version":"3.55.0"},"reference-count":66,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2020,11,5]],"date-time":"2020-11-05T00:00:00Z","timestamp":1604534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100012155","name":"National Institute for Medical Research Development","doi-asserted-by":"publisher","award":["973166"],"award-info":[{"award-number":["973166"]}],"id":[{"id":"10.13039\/501100012155","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Early exacerbation of cutaneous leishmaniasis is mainly affected by both the salivary and Leishmania parasite components. Little is known of the vaccine combination made by immunogenic proteins of sandfly saliva (SP15) with Leishmania parasites (LeIF) as a single prophylactic vaccine, namely SaLeish. Also, there are no data available to determine the species-specific sequence of SP15 isolated from the Iranian Phlebotomus papatasi.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Integrated bioinformatics and genetic engineering methods were employed to design, optimize and obtain a vector\u2013parasite-based vaccine formulation in a whole-length fusion form of LeIF-SP15 against leishmaniasis. Holistic gene optimization was initially performed to obtain a high yield of pure \u2018whole-SaLeish\u2019 expression using bioinformatics analyses. Genomic and salivary gland RNAs of wild-caught P. papatasi were extracted and their complementary DNA was amplified and cloned into pJET vector.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The new chimeric protein of whole-SaLeish and randomly selected transcripts of native PpIRSP15 (GenBank accession nos. MT025054 and MN938854, MN938855 and MN938856) were successfully expressed, purified and validated by immunoblotting assay. Furthermore, despite the single amino acid polymorphisms of PpIRSP15 found at positions Y23 and E73 within the population of wild Iranian sandflies, antigenicity and conservancy of PpIRSP15 epitopes remained constant to activate T cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The SaLeish vaccine strategy takes advantage of a plethora of vector\u2013parasite immunogenic proteins with potential protective efficacy to stimulate both the innate and specific cellular immune responses against Leishmania parasites.<\/jats:p><\/jats:sec>","DOI":"10.1093\/trstmh\/traa119","type":"journal-article","created":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T19:13:27Z","timestamp":1602875607000},"page":"699-713","source":"Crossref","is-referenced-by-count":4,"title":["Cloning, high-level gene expression and bioinformatics analysis of SP15 and LeIF from<i>Leishmania major<\/i>and Iranian<i>Phlebotomus papatasi<\/i>saliva as single and novel fusion proteins: a potential vaccine candidate against leishmaniasis"],"prefix":"10.1093","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5984-7287","authenticated-orcid":false,"given":"Ali","family":"Bordbar","sequence":"first","affiliation":[{"name":"Molecular Systematics Laboratory, Parasitology Department, Pasteur Institute of Iran, 69 Pasteur Ave., Tehran, Iran"},{"name":"Venom and Biotherapeutics Molecules Laboratory, Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8559-1668","authenticated-orcid":false,"given":"Massoud","family":"Amanlou","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry, Faculty of Pharmacy and Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0125-3918","authenticated-orcid":false,"given":"Kamran","family":"Pooshang Bagheri","sequence":"additional","affiliation":[{"name":"Venom and Biotherapeutics Molecules Laboratory, Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul Donald","family":"Ready","sequence":"additional","affiliation":[{"name":"Department of Disease Control, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahar","family":"Ebrahimi","sequence":"additional","affiliation":[{"name":"Molecular Systematics Laboratory, Parasitology Department, Pasteur Institute of Iran, 69 Pasteur Ave., Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Shahbaz Mohammadi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Genetics and Metabolism Research Group, Pasteur Institute of Iran, Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyedeh Maryam","family":"Ghafari","sequence":"additional","affiliation":[{"name":"Molecular Systematics Laboratory, Parasitology Department, Pasteur Institute of Iran, 69 Pasteur Ave., Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6400-1254","authenticated-orcid":false,"given":"Parviz","family":"Parvizi","sequence":"additional","affiliation":[{"name":"Molecular Systematics Laboratory, Parasitology Department, Pasteur Institute of Iran, 69 Pasteur Ave., Tehran, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,11,5]]},"reference":[{"key":"2021060200005560500_bib1"},{"key":"2021060200005560500_bib2"},{"issue":"5","key":"2021060200005560500_bib3","doi-asserted-by":"crossref","first-page":"e35671","DOI":"10.1371\/journal.pone.0035671","article-title":"Leishmaniasis worldwide and global estimates of its incidence","volume":"7","author":"Alvar","year":"2012","journal-title":"PLoS One"},{"issue":"10","key":"2021060200005560500_bib4","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1111\/j.1469-0691.2011.03630.x","article-title":"Leishmaniasis chemotherapy\u2014challenges and opportunities","volume":"17","author":"Croft","year":"2011","journal-title":"Clin Microbiol Infect"},{"issue":"8","key":"2021060200005560500_bib5","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1016\/j.drudis.2015.04.006","article-title":"Potential therapeutic targets and the role of technology in developing novel antileishmanial drugs","volume":"20","author":"Rajasekaran","year":"2015","journal-title":"Drug Discov Today"},{"key":"2021060200005560500_bib6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jim.2015.03.017","article-title":"Vaccines for visceral leishmaniasis: a review","volume":"422","author":"Jain","year":"2015","journal-title":"J Immunol Methods"},{"issue":"25","key":"2021060200005560500_bib7","doi-asserted-by":"crossref","first-page":"2779","DOI":"10.1016\/j.vaccine.2016.04.067","article-title":"Strategic evaluation of vaccine candidate antigens for the prevention of visceral leishmaniasis","volume":"34","author":"Duthie","year":"2016","journal-title":"Vaccine"},{"key":"2021060200005560500_bib8","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/j.meegid.2014.07.028","article-title":"What's behind a sand fly bite? The profound effect of sand fly saliva on host hemostasis, inflammation and immunity","volume":"28","author":"Abdeladhim","year":"2014","journal-title":"Infect Genet Evol"},{"issue":"7","key":"2021060200005560500_bib9","doi-asserted-by":"crossref","first-page":"e0005600","DOI":"10.1371\/journal.pntd.0005600","article-title":"Insights into the sand fly saliva: blood-feeding and immune interactions between sand flies, hosts, and Leishmania","volume":"11","author":"Lestinova","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"issue":"2","key":"2021060200005560500_bib10","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1016\/S1286-4579(00)01331-9","article-title":"The biological and immunomodulatory properties of sand fly saliva and its role in the establishment of Leishmania infections","volume":"14","author":"Kamhawi","year":"2000","journal-title":"Microbes Infect"},{"issue":"9","key":"2021060200005560500_bib11","doi-asserted-by":"crossref","first-page":"4648","DOI":"10.1128\/IAI.00394-07","article-title":"Leish-111f, a recombinant polyprotein vaccine that protects against visceral leishmaniasis by elicitation of CD4+ T cells","volume":"75","author":"Coler","year":"2007","journal-title":"Infect Immun"},{"key":"2021060200005560500_bib12","article-title":"Study to evaluate the Leish-111f\u00a0+\u00a0MPL-SE vaccine in healthy adults not previously exposed to Leishmania parasite","author":"Piazza"},{"key":"2021060200005560500_bib13","doi-asserted-by":"crossref","first-page":"104189","DOI":"10.1016\/j.meegid.2020.104189","article-title":"Bioinformatics analyses of immunogenic T-cell epitopes of LeIF and PpSP15 proteins from Leishmania major and sand fly saliva used as model antigens for the design of a multi-epitope vaccine to control leishmaniasis","volume":"80","author":"Bordbar","year":"2020","journal-title":"Infect Genet Evol"},{"issue":"21","key":"2021060200005560500_bib14","doi-asserted-by":"crossref","first-page":"5904","DOI":"10.1016\/j.bmc.2017.07.013","article-title":"In silico analysis and in vitro evaluation of immunogenic and immunomodulatory properties of promiscuous peptides derived from Leishmania infantum eukaryotic initiation factor","volume":"25","author":"Koutsoni","year":"2017","journal-title":"Bioorg Med Chem"},{"issue":"5","key":"2021060200005560500_bib15","doi-asserted-by":"crossref","first-page":"e97319","DOI":"10.1371\/journal.pone.0097319","article-title":"Leishmania eukaryotic initiation factor (LeIF) inhibits parasite growth in murine macrophages","volume":"9","author":"Koutsoni","year":"2014","journal-title":"PLoS One"},{"key":"2021060200005560500_bib16","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.imlet.2019.04.001","article-title":"Leishmania infantum LeIF and its recombinant polypeptides induce the maturation of dendritic cells in vitro: an insight for dendritic cells based vaccine","volume":"210","author":"Barhoumi","year":"2019","journal-title":"Immunol Lett"},{"issue":"10","key":"2021060200005560500_bib17","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1111\/j.1365-3024.2011.01320.x","article-title":"Leishmania infantum LeIF and its recombinant polypeptides modulate interleukin IL-12p70, IL-10 and tumour necrosis factor-\u03b1 production by human monocytes","volume":"33","author":"Barhoumi","year":"2011","journal-title":"Parasite Immunol"},{"key":"2021060200005560500_bib18","doi-asserted-by":"crossref","first-page":"2634","DOI":"10.1002\/eji.1830271024","article-title":"A Leishmania protein that modulates interleukin (IL)-12, IL-10 and tumor necrosis factor-\u03b1 production and expression of B7-1 in human monocyte-derived antigen-presenting cells","volume":"27","author":"Probst","year":"1997","journal-title":"Eur J Immunol"},{"issue":"5","key":"2021060200005560500_bib19","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1078\/0171-2985-00105","article-title":"Dendritic cell-based vaccination strategies: induction of protective immunity against leishmaniasis","volume":"204","author":"Moll","year":"2011","journal-title":"Immunobiology"},{"issue":"2","key":"2021060200005560500_bib20","doi-asserted-by":"crossref","first-page":"896","DOI":"10.4049\/jimmunol.165.2.896","article-title":"IL-12 is required to maintain a Th1 response during Leishmania major infection","volume":"165","author":"Park","year":"2000","journal-title":"J Immunol"},{"issue":"2","key":"2021060200005560500_bib21","doi-asserted-by":"crossref","first-page":"135","DOI":"10.4103\/0974-777X.62876","article-title":"Immunological perspectives of leishmaniasis","volume":"2","author":"Nylen","year":"2010","journal-title":"J Glob Infect Dis"},{"issue":"4","key":"2021060200005560500_bib22","doi-asserted-by":"crossref","first-page":"e2781","DOI":"10.1371\/journal.pntd.0002781","article-title":"Characterization of the early inflammatory infiltrate at the feeding site of infected sand flies in mice protected from vector-transmitted Leishmania major by exposure to uninfected bites","volume":"8","author":"Teixeira","year":"2014","journal-title":"PLoS Negl Trop Dis"},{"issue":"290","key":"2021060200005560500_bib23","doi-asserted-by":"crossref","first-page":"290ra90","DOI":"10.1126\/scitranslmed.aaa3043","article-title":"A sand fly salivary protein vaccine shows efficacy against vector-transmitted cutaneous leishmaniasis in nonhuman primates","volume":"7","author":"Oliveira","year":"2015","journal-title":"Sci Transl Med"},{"issue":"3","key":"2021060200005560500_bib24","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1084\/jem.194.3.331","article-title":"Toward a defined anti-Leishmania vaccine targeting vector antigens: characterization of a protective salivary protein","volume":"194","author":"Valenzuela","year":"2001","journal-title":"J Exp Med"},{"issue":"12","key":"2021060200005560500_bib25","doi-asserted-by":"crossref","first-page":"2735","DOI":"10.1038\/jid.2012.205","article-title":"Immunity to sand fly salivary protein LJM11 modulates host response to vector-transmitted Leishmania conferring ulcer-free protection","volume":"132","author":"Gomes","year":"2012","journal-title":"J Invest Dermatol"},{"issue":"22","key":"2021060200005560500_bib26","doi-asserted-by":"crossref","first-page":"7845","DOI":"10.1073\/pnas.0712153105","article-title":"Immunity to a salivary protein of a sand fly vector protects against the fatal outcome of visceral leishmaniasis in a hamster model","volume":"105","author":"Gomes","year":"2008","journal-title":"Proc Natl Acad Sci USA"},{"issue":"4","key":"2021060200005560500_bib27","doi-asserted-by":"crossref","first-page":"e226","DOI":"10.1371\/journal.pntd.0000226","article-title":"Immunity to distinct sand fly salivary proteins primes the anti-Leishmania immune response towards protection or exacerbation of disease","volume":"2","author":"Oliveira","year":"2008","journal-title":"PLoS Negl Trop Dis"},{"issue":"1","key":"2021060200005560500_bib28","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1186\/s13071-015-0914-2","article-title":"Phlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations","volume":"8","author":"Ramalho-Ortig\u00e3o","year":"2015","journal-title":"Parasite Vector"},{"key":"2021060200005560500_bib29","doi-asserted-by":"crossref","first-page":"290","DOI":"10.3389\/fcimb.2018.00290","article-title":"Comparative evolution of sand fly salivary protein families and implications for biomarkers of vector exposure and salivary vaccine candidates","volume":"8","author":"Coutinho-Abreu","year":"2018","journal-title":"Front Cell Infect Microbiol."},{"issue":"1","key":"2021060200005560500_bib30","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1186\/1471-2164-7-226","article-title":"High degree of conservancy among secreted salivary gland proteins from two geographically distant Phlebotomus duboscqi sandflies populations (Mali and Kenya)","volume":"7","author":"Kato","year":"2006","journal-title":"BMC Genomics"},{"issue":"11","key":"2021060200005560500_bib31","doi-asserted-by":"crossref","first-page":"6171","DOI":"10.4049\/jimmunol.161.11.6171","article-title":"LeIF: a recombinant Leishmania protein that induces an IL-12-mediated Th1 cytokine profile","volume":"161","author":"Skeiky","year":"1998","journal-title":"J Immunol"},{"issue":"10","key":"2021060200005560500_bib32","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1086\/344733","article-title":"Seroconversion against Lutzomyia longipalpis saliva concurrent with the development of anti-Leishmania chagasi delayed-type hypersensitivity","volume":"186","author":"Gomes","year":"2002","journal-title":"J Infect Dis"},{"issue":"11","key":"2021060200005560500_bib33","doi-asserted-by":"crossref","first-page":"3111","DOI":"10.1002\/eji.200737431","article-title":"Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis","volume":"37","author":"Vinhas","year":"2007","journal-title":"Eur J Immunol"},{"issue":"19","key":"2021060200005560500_bib34","doi-asserted-by":"crossref","first-page":"3531","DOI":"10.1016\/j.vaccine.2011.02.096","article-title":"A clinical trial to evaluate the safety and immunogenicity of the LEISH-F1+ MPL-SE vaccine for use in the prevention of visceral leishmaniasis","volume":"29","author":"Chakravarty","year":"2011","journal-title":"Vaccine"},{"key":"2021060200005560500_bib35","first-page":"121","article-title":"A taxonomic review of the genus Phlebotomus (Diptera: Psychodidae)","volume-title":"Bull Br Museum","author":"Lewis","year":"1982"},{"issue":"1","key":"2021060200005560500_bib36","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.pep.2012.02.013","article-title":"Engineering genes for predictable protein expression","volume":"83","author":"Gustafsson","year":"2012","journal-title":"Protein Expr Purif"},{"issue":"1","key":"2021060200005560500_bib37","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/nrm.2017.91","article-title":"Codon optimality, bias and usage in translation and mRNA decay","volume":"19","author":"Hanson","year":"2018","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"Suppl 2","key":"2021060200005560500_bib38","doi-asserted-by":"crossref","first-page":"W126","DOI":"10.1093\/nar\/gkm219","article-title":"OPTIMIZER: a web server for optimizing the codon usage of DNA sequences","volume":"35","author":"Puigbo","year":"2007","journal-title":"Nucleic Acids Res"},{"issue":"Suppl 1","key":"2021060200005560500_bib39","doi-asserted-by":"crossref","first-page":"D524","DOI":"10.1093\/nar\/gkm831","article-title":"HEG-DB: a database of predicted highly expressed genes in prokaryotic complete genomes under translational selection","volume":"36","author":"Puigbo","year":"2007","journal-title":"Nucleic Acids Res"},{"issue":"112","key":"2021060200005560500_bib40","first-page":"531","article-title":"Protein identification and analysis tools in the ExPASy server","author":"Wilkins","year":"1999","journal-title":"Methods Mol Biol"},{"key":"2021060200005560500_bib41","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1385\/1-59259-890-0:571","article-title":"Protein identification and analysis tools on the ExPASy server","volume-title":"The Proteomics Protocols Handbook","author":"Gasteiger","year":"2005"},{"issue":"3","key":"2021060200005560500_bib42","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1006\/jmbi.2000.4315","article-title":"Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes","volume":"305","author":"Krogh","year":"2001","journal-title":"J Mol Biol"},{"issue":"13","key":"2021060200005560500_bib43","doi-asserted-by":"crossref","first-page":"3406","DOI":"10.1093\/nar\/gkg595","article-title":"Mfold web server for nucleic acid folding and hybridization prediction","volume":"31","author":"Zuker","year":"2003","journal-title":"Nucleic Acids Res"},{"issue":"W1","key":"2021060200005560500_bib44","doi-asserted-by":"crossref","first-page":"W402","DOI":"10.1093\/nar\/gkz297","article-title":"The PSIPRED protein analysis workbench: 20 years on","volume":"47","author":"Buchan","year":"2019","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"2021060200005560500_bib45","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1093\/nar\/9.1.133","article-title":"Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information","volume":"9","author":"Zuker","year":"1981","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"2021060200005560500_bib46","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1073\/pnas.95.4.1460","article-title":"A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics","volume":"95","author":"Santa Lucia","year":"1998","journal-title":"Proc Natl Acad Sci USA"},{"issue":"Suppl 2","key":"2021060200005560500_bib47","doi-asserted-by":"crossref","first-page":"W70","DOI":"10.1093\/nar\/gkn188","article-title":"The Vienna RNA websuite","volume":"36","author":"Gruber","year":"2008","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"2021060200005560500_bib48","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/1748-7188-6-26","article-title":"ViennaRNA package 2.0","volume":"6","author":"Lorenz","year":"2011","journal-title":"Algorithm Mol Biol"},{"issue":"Suppl 2","key":"2021060200005560500_bib49","doi-asserted-by":"crossref","first-page":"W230","DOI":"10.1093\/nar\/gki412","article-title":"DiANNA: a web server for disulfide connectivity prediction","volume":"33","author":"Ferr\u00e8","year":"2005","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"2021060200005560500_bib50","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1038\/nmeth.3213","article-title":"The I-TASSER suite: protein structure and function prediction","volume":"12","author":"Yang","year":"2015","journal-title":"Nat Methods"},{"key":"2021060200005560500_bib51","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/1471-2105-9-40","article-title":"I-TASSER server for protein 3D structure prediction","volume":"9","author":"Zhang","year":"2008","journal-title":"BMC Bioinformatics"},{"issue":"2","key":"2021060200005560500_bib52","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1002\/prot.21968","article-title":"Specific interactions for ab initio folding of protein terminal regions with secondary structures","volume":"72","author":"Yang","year":"2008","journal-title":"Proteins"},{"issue":"13","key":"2021060200005560500_bib53","doi-asserted-by":"crossref","first-page":"3252","DOI":"10.1002\/j.1460-2075.1995.tb07328.x","article-title":"The stability of Escherichia coli lacZ mRNA depends upon the simultaneity of its synthesis and translation","volume":"14","author":"Iost","year":"1995","journal-title":"EMBO J"},{"key":"2021060200005560500_bib54","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/S0076-6879(05)94008-1","article-title":"Robotic cloning and protein production platform of the Northeast Structural Genomics Consortium","volume":"394","author":"Acton","year":"2005","journal-title":"Methods Enzymol"},{"issue":"1","key":"2021060200005560500_bib55","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1002\/0471142727.mb1001as76","article-title":"Spectrophotometric and colorimetric determination of protein concentration","volume":"76","author":"Simonian","year":"2006","journal-title":"Curr Protoc Mol Biol"},{"issue":"7586","key":"2021060200005560500_bib56","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1038\/nature16509","article-title":"Codon influence on protein expression in E. coli correlates with mRNA levels","volume":"529","author":"Bo\u00ebl","year":"2016","journal-title":"Nature"},{"issue":"11","key":"2021060200005560500_bib57","doi-asserted-by":"crossref","first-page":"e47347","DOI":"10.1371\/journal.pone.0047347","article-title":"Updating the salivary gland transcriptome of Phlebotomus papatasi (Tunisian strain): the search for sand fly-secreted immunogenic proteins for humans","volume":"7","author":"Abdeladhim","year":"2012","journal-title":"PLoS One"},{"issue":"2","key":"2021060200005560500_bib58","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1111\/j.1365-2583.2004.00539.x","article-title":"Genetic variation in the sand fly salivary protein, SP-15, a potential vaccine candidate against Leishmania major","volume":"14","author":"Elnaiem","year":"2005","journal-title":"Insect Mol Biol"},{"issue":"21","key":"2021060200005560500_bib59","doi-asserted-by":"crossref","first-page":"3717","DOI":"10.1242\/jeb.01185","article-title":"Identification of the most abundant secreted proteins from the salivary glands of the sand fly Lutzomyia longipalpis, vector of Leishmania chagasi","volume":"207","author":"Valenzuela","year":"2004","journal-title":"J Exp Biol"},{"issue":"5","key":"2021060200005560500_bib60","doi-asserted-by":"crossref","first-page":"e1000441","DOI":"10.1371\/journal.ppat.1000441","article-title":"Sand fly salivary proteins induce strong cellular immunity in a natural reservoir of visceral leishmaniasis with adverse consequences for Leishmania","volume":"5","author":"Collin","year":"2009","journal-title":"PLoS Pathog"},{"key":"2021060200005560500_bib61","doi-asserted-by":"crossref","first-page":"110","DOI":"10.3389\/fimmu.2012.00110","article-title":"The immune response to sand fly salivary proteins and its influence on Leishmania immunity","volume":"3","author":"Gomes","year":"2012","journal-title":"Front Immunol"},{"issue":"11","key":"2021060200005560500_bib62","doi-asserted-by":"crossref","first-page":"7160","DOI":"10.4049\/jimmunol.174.11.7160","article-title":"Kinetoplastid membrane protein-11 DNA vaccination induces complete protection against both pentavalent antimonial-sensitive and-resistant strains of Leishmania donovani that correlates with inducible nitric oxide synthase activity and IL-4 generation: evidence for mixed Th1-and Th2-like responses in visceral leishmaniasis","volume":"174","author":"Basu","year":"2005","journal-title":"J Immunol"},{"key":"2021060200005560500_bib63","doi-asserted-by":"crossref","first-page":"165","DOI":"10.3389\/fpubh.2014.00165","article-title":"The role of CD4 and CD8 T cells in human cutaneous leishmaniasis","volume":"2","author":"da Silva Santos","year":"2014","journal-title":"Front Public Health"},{"issue":"22","key":"2021060200005560500_bib64","doi-asserted-by":"crossref","first-page":"5086","DOI":"10.1111\/j.1742-4658.2006.05506.x","article-title":"Leishmania infantum LeIF protein is an ATP-dependent RNA helicase and an eIF4A-like factor that inhibits translation in yeast","volume":"273","author":"Barhoumi","year":"2006","journal-title":"FEBS J"},{"issue":"10","key":"2021060200005560500_bib65","doi-asserted-by":"crossref","first-page":"2207","DOI":"10.1016\/j.vaccine.2009.12.055","article-title":"Intranasal immunization with Leish-111f induces IFN-\u03b3 production and protects mice from Leishmania major infection","volume":"28","author":"Sakai","year":"2010","journal-title":"Vaccine"},{"issue":"3","key":"2021060200005560500_bib66","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.trsl.2013.03.006","article-title":"New vaccines for neglected parasitic diseases and dengue","volume":"162","author":"Beaumier","year":"2013","journal-title":"Transl Res"}],"container-title":["Transactions of The Royal Society of Tropical Medicine and Hygiene"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/trstmh\/article-pdf\/115\/6\/699\/38384470\/traa119.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/trstmh\/article-pdf\/115\/6\/699\/38384470\/traa119.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T22:45:44Z","timestamp":1696891544000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/trstmh\/article\/115\/6\/699\/5957303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,5]]},"references-count":66,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,11,5]]},"published-print":{"date-parts":[[2021,6,2]]}},"URL":"https:\/\/doi.org\/10.1093\/trstmh\/traa119","relation":{},"ISSN":["0035-9203","1878-3503"],"issn-type":[{"value":"0035-9203","type":"print"},{"value":"1878-3503","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,6]]},"published":{"date-parts":[[2020,11,5]]}}}