{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:49:38Z","timestamp":1740138578562,"version":"3.37.3"},"reference-count":5,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T00:00:00Z","timestamp":1624838400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["ERC-CoG 864180"],"award-info":[{"award-number":["ERC-CoG 864180"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill & Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1173572"],"award-info":[{"award-number":["OPP1173572"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Direct membrane feeding assays assess the transmission potential of malaria-infected individuals using whole blood collected in anticoagulant vacutainers.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The potential inhibitory effect of four commonly used anticoagulants on gametocyte infectivity to mosquitoes was assessed in standard membrane feeding assays with cultured Plasmodium falciparum.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Infection burden in mosquitoes was significantly reduced when blood was collected in sodium citrate and EDTA. Transmission was highest when blood was collected in lithium heparin and sodium heparin, although a concentration-dependent inhibition of mosquito infection was also observed.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Although anticoagulants can reduce transmission efficiency, lithium heparin and sodium heparin are the best anticoagulants for evaluating malaria transmission.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/trstmh\/trab095","type":"journal-article","created":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T19:12:41Z","timestamp":1623697961000},"page":"187-189","source":"Crossref","is-referenced-by-count":4,"title":["The effect of anticoagulants in blood collection tubes on <i>Plasmodium falciparum<\/i> transmission in direct membrane feeding assays"],"prefix":"10.1093","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3952-6491","authenticated-orcid":false,"given":"Wouter","family":"Graumans","sequence":"first","affiliation":[{"name":"Radboud University Medical Center , Radboud Institute for Health Sciences , Department of Medical Microbiology, 268, PO Box 9101, 6500 HB Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kjerstin","family":"Lanke","sequence":"additional","affiliation":[{"name":"Radboud University Medical Center , Radboud Institute for Health Sciences , Department of Medical Microbiology, 268, PO Box 9101, 6500 HB Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geert-Jan","family":"van Gemert","sequence":"additional","affiliation":[{"name":"Radboud University Medical Center , Radboud Institute for Health Sciences , Department of Medical Microbiology, 268, PO Box 9101, 6500 HB Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manon","family":"Alkema","sequence":"additional","affiliation":[{"name":"Radboud University Medical Center , Radboud Institute for Health Sciences , Department of Medical Microbiology, 268, PO Box 9101, 6500 HB Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marga","family":"van de Vegte-Bolmer","sequence":"additional","affiliation":[{"name":"Radboud University Medical Center , Radboud Institute for Health Sciences , Department of Medical Microbiology, 268, PO Box 9101, 6500 HB Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teun","family":"Bousema","sequence":"additional","affiliation":[{"name":"Radboud University Medical Center , Radboud Institute for Health Sciences , Department of Medical Microbiology, 268, PO Box 9101, 6500 HB Nijmegen, The Netherlands"},{"name":"Department of Immunology and Infection, London School of Hygiene and Tropical Medicine, Keppel St, London WC1E 7HT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7080-2215","authenticated-orcid":false,"given":"Katharine A","family":"Collins","sequence":"additional","affiliation":[{"name":"Radboud University Medical Center , Radboud Institute for Health Sciences , Department of Medical Microbiology, 268, PO Box 9101, 6500 HB Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,6,28]]},"reference":[{"issue":"4","key":"2022031008060786100_bib1","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1172\/JCI98012","article-title":"A controlled human malaria infection model enabling evaluation of transmission-blocking interventions","volume":"128","author":"Collins","year":"2018","journal-title":"J Clin Invest"},{"key":"2022031008060786100_bib2","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.1093\/infdis\/jiz680","article-title":"P. falciparum gametocyte density and infectivity in peripheral blood and skin tissue of naturally infected parasite carriers in Burkina Faso","volume":"223","author":"Meibalan","year":"2021","journal-title":"J Infect Dis"},{"issue":"8","key":"2022031008060786100_bib3","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.3390\/biom10081136","article-title":"Heparin administered to Anopheles in membrane feeding assays blocks Plasmodium development in the mosquito","volume":"10","author":"Lantero","year":"2020","journal-title":"Biomolecules"},{"issue":"2","key":"2022031008060786100_bib4","doi-asserted-by":"crossref","first-page":"242","DOI":"10.4269\/ajtmh.2007.77.242","article-title":"Effects of anticoagulants on Plasmodium vivax oocyst development in Anopheles albimanus mosquitoes","volume":"77","author":"Solarte","year":"2007","journal-title":"Am J Trop Med Hyg"},{"issue":"Pt 2","key":"2022031008060786100_bib5","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1017\/S0031182000062065","article-title":"Infectivity of cultured Plasmodium falciparum gametocytes to mosquitoes","volume":"98","author":"Ponnudurai","year":"1989","journal-title":"Parasitology"}],"container-title":["Transactions of The Royal Society of Tropical Medicine and Hygiene"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/trstmh\/article-pdf\/116\/2\/187\/42793773\/trab095.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/trstmh\/article-pdf\/116\/2\/187\/42793773\/trab095.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,10]],"date-time":"2022-03-10T08:08:30Z","timestamp":1646899710000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/trstmh\/article\/116\/2\/187\/6310906"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,28]]},"references-count":5,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021,6,28]]},"published-print":{"date-parts":[[2022,2,1]]}},"URL":"https:\/\/doi.org\/10.1093\/trstmh\/trab095","relation":{},"ISSN":["0035-9203","1878-3503"],"issn-type":[{"type":"print","value":"0035-9203"},{"type":"electronic","value":"1878-3503"}],"subject":[],"published-other":{"date-parts":[[2022,2]]},"published":{"date-parts":[[2021,6,28]]}}}