{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:49:52Z","timestamp":1740138592475,"version":"3.37.3"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T00:00:00Z","timestamp":1644451200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100005671","name":"Funda\u00e7\u00e3o de Apoio \u00e0 Pesquisa e Inova\u00e7\u00e3o Tecnol\u00f3gica do Estado de Sergipe","doi-asserted-by":"publisher","award":["10\/2016"],"award-info":[{"award-number":["10\/2016"]}],"id":[{"id":"10.13039\/501100005671","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005671","name":"FAPITEC\/SE","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005671","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Dengue virus (DENV) and Zika virus (ZIKV) have a severe impact on human health worldwide. To understand the dynamics of these viruses in mosquito populations, it is necessary to maintain surveillance during non-epidemic years.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We aimed to assess the presence of DENV and ZIKV in Aedes aegypti females in the state of Sergipe, northeastern Brazil, during a non-epidemic year. The Ae. aegypti females collected were sectioned, and the heads and thorax were used to analyze the infection rate. Each female was first analyzed to detect Flavivirus using RT-PCR. Flavivirus-positive samples were further screened to detect ZIKV and DENV types 1, 2, 3 and 4.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>A total of 184 females were collected. ZIKV was identified in 5.4% of the sample and DENV in 7.1%, with the DENV-positive samples belonging to subtypes 2, 3 and 4. The presence of coinfected vectors was also observed. Of the four cities tested, only one was negative for all viruses.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>These results show that the maintenance of vigilance during non-epidemic years can provide data on viruses circulating before the onset of outbreaks. This can enable the planning and implementation of local control measures to prevent the appearance of new outbreaks.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/trstmh\/trac009","type":"journal-article","created":{"date-parts":[[2022,1,26]],"date-time":"2022-01-26T20:19:45Z","timestamp":1643228385000},"page":"924-929","source":"Crossref","is-referenced-by-count":4,"title":["Silent circulation of Zika and dengue virus in <i>Aedes aegypti<\/i> (Diptera: Culicidae) during a non-epidemic year in the state of Sergipe, northeastern Brazil"],"prefix":"10.1093","volume":"116","author":[{"given":"Myrela C S","family":"Jesus","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biologia Parasit\u00e1ria, Universidade Federal de Sergipe , S\u00e3o Crist\u00f3v\u00e3o 49100-000, Sergipe, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rynat D O","family":"Chagas","sequence":"additional","affiliation":[{"name":"Departamento de Medicina, Universidade Federal de Sergipe , Aracaju 49060-108, Sergipe, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matheus L J","family":"Santos","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Fisiol\u00f3gicas, Universidade Federal de Sergipe , S\u00e3o Crist\u00f3v\u00e3o 49100-000, Sergipe, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafaela W F","family":"Santos","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biologia Parasit\u00e1ria, Universidade Federal de Sergipe , S\u00e3o Crist\u00f3v\u00e3o 49100-000, Sergipe, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5017-3725","authenticated-orcid":false,"given":"Roseli","family":"La Corte","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biologia Parasit\u00e1ria, Universidade Federal de Sergipe , S\u00e3o Crist\u00f3v\u00e3o 49100-000, Sergipe, Brasil"},{"name":"Departamento de Morfologia, Universidade Federal de Sergipe , S\u00e3o Crist\u00f3v\u00e3o 49100-000, Sergipe, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2305-7996","authenticated-orcid":false,"given":"Luciane M","family":"Storti-Melo","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biologia Parasit\u00e1ria, Universidade Federal de Sergipe , S\u00e3o Crist\u00f3v\u00e3o 49100-000, Sergipe, Brasil"},{"name":"Departamento de Biologia, Universidade Federal de Sergipe , S\u00e3o Crist\u00f3v\u00e3o 49100-000, Sergipe, Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,2,10]]},"reference":[{"issue":"5","key":"2022100209511191600_bib1","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1097\/OGX.0000000000000433","article-title":"Mosquito-borne diseases as a global health problem","volume":"72","author":"Diouf","year":"2017","journal-title":"Obstet Gynecol Surv"},{"issue":"6","key":"2022100209511191600_bib2","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.4269\/ajtmh.17-0866","article-title":"Mosquito-borne human viral diseases: why aedes aegypti?","volume":"98","author":"Powell","year":"2018","journal-title":"Am J Trop Med Hyg"},{"issue":"6","key":"2022100209511191600_bib3","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/S1473-3099(16)00026-8","article-title":"Minist\u00e9rio da sa\u00fade. The global burden of dengue: an analysis from the Global Burden of Disease study 2013","volume":"16","author":"Stanaway","year":"2016","journal-title":"Lancet Infect Dis"},{"journal-title":"World Health Organization, Dengue","year":"2016","author":"WHO","key":"2022100209511191600_bib4"},{"issue":"2","key":"2022100209511191600_bib5","doi-asserted-by":"crossref","first-page":"e0228346","DOI":"10.1371\/journal.pone.0228346","article-title":"Spatial and temporal distribution of dengue in Brazil, 1990\u20132017","volume":"15","author":"Andrioli","year":"2020","journal-title":"PLoS ONE"},{"key":"2022100209511191600_bib6","first-page":"1","article-title":"Potential mechanisms of Zika-linked microcephaly","author":"Merfeld","year":"2017","journal-title":"Rev Dev Biol"},{"key":"2022100209511191600_bib7","doi-asserted-by":"crossref","first-page":"3","DOI":"10.4103\/0974-777X.176140","article-title":"The emergence of Zika virus as a global health security threat: a review and a consensus statement of the INDUSEM Joint Working Group (JWG)","volume":"8","author":"Sikka","year":"2016","journal-title":"J Glob Infect Dis"},{"article-title":"Informe epidemiol\u00f3gico No 57 \u2013 semana epidemiol\u00f3gica (Se) 52\/2016 (25 a 31\/12\/2016) monitoramento dos casos de microcefalia no Brasil","year":"2017","author":"BRASIL","key":"2022100209511191600_bib8"},{"key":"2022100209511191600_bib9","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1055\/s-0037-1603450","article-title":"Zika virus infection in pregnant women and microcephaly","volume":"39","author":"Duarte","year":"2017","journal-title":"Rev Bras Ginecol Obstet"},{"issue":"1","key":"2022100209511191600_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-019-3461-4","article-title":"Zika virus detection, isolation and genome sequencing through culicidae sampling during the epidemic in vit\u00f3ria, esp\u00edrito santo, brazil","volume":"12","author":"Ayres","year":"2019","journal-title":"Parasit Vector"},{"key":"2022100209511191600_bib11","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.actatropica.2017.07.014","article-title":"Dengue serotype circulation in natural populations of Aedes aegypti","volume":"176","author":"dos Santos","year":"2017","journal-title":"Acta Trop"},{"issue":"10","key":"2022100209511191600_bib12","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1590\/0074-02760160332","article-title":"First detection of natural infection of Aedes aegypti with Zika virus in Brazil and throughout South America","volume":"111","author":"Ferreira-de-Brito","year":"2016","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"1","key":"2022100209511191600_bib13","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1186\/1756-3305-6-253","article-title":"Detection of the V1016G mutation in the voltage-gated sodium channel gene of Aedes aegypti (Diptera: culicidae) by allele-specific PCR assay, and its distribution and effect on deltamethrin resistance in Thailand","volume":"6","author":"Stenhouse","year":"2013","journal-title":"Parasit Vectors"},{"issue":"1-2","key":"2022100209511191600_bib14","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.jviromet.2010.05.006","article-title":"Screening of mosquitoes using SYBR green I-based real-time RT-PCR with group-specific primers for detection of flaviviruses and alphaviruses in Taiwan","volume":"168","author":"Yang","year":"2010","journal-title":"J Virol Methods"},{"key":"2022100209511191600_bib15","first-page":"49","article-title":"Medidas dos n\u00edveis de infesta\u00e7\u00e3o urbana para aedes (stegomyia) aegypti e aedes (stegomyia) albopictus em programa de vigil\u00e2ncia entomol\u00f3gica","volume-title":"Informe Epidemiol\u00f3gico do SUS","author":"Gomes","year":"1998"},{"key":"2022100209511191600_bib16","first-page":"113","article-title":"Aedes aegypti: hist\u00f3rico do controle no Brasil","volume":"16","author":"Braga","year":"2007","journal-title":"Epidemiol Serv Sa\u00fade"},{"issue":"1","key":"2022100209511191600_bib17","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1186\/s13071-018-2830-8","article-title":"History, epidemiology and diagnostics of dengue in the American and Brazilian contexts: a review","volume":"11","author":"Salles","year":"2018","journal-title":"Parasit Vectors"},{"issue":"2","key":"2022100209511191600_bib18","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1590\/S1415-790X2013000200002","article-title":"A ocorr\u00eancia da dengue e varia\u00e7\u00f5es meteorol\u00f3gicas no Brasil: revis\u00e3o sistem\u00e1tica","volume":"16","author":"Viana","year":"2013","journal-title":"Rev Bras Epidemiol"},{"year":"2018","author":"SERGIPE","key":"2022100209511191600_bib19"},{"year":"2017","author":"BRASIL","key":"2022100209511191600_bib20"},{"year":"2019","author":"BRASIL","key":"2022100209511191600_bib21"},{"issue":"1","key":"2022100209511191600_bib22","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.jviromet.2008.09.023","article-title":"A retrospective survey of dengue virus infection in fatal cases from an epidemic in Brazil","volume":"155","author":"de Ara\u00fajo","year":"2009","journal-title":"J Virol Methods"},{"issue":"10","key":"2022100209511191600_bib23","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.3201\/eid1410.071164","article-title":"Recent shift in age pattern of dengue hemorrhagic fever, Brazil","volume":"14","author":"Teixeira","year":"2008","journal-title":"Emerg Infect Dis"},{"issue":"3","key":"2022100209511191600_bib24","doi-asserted-by":"crossref","first-page":"449","DOI":"10.4269\/ajtmh.2006.74.449","article-title":"Serotype-specific differences in clinical manifestations of dengue","volume":"74","author":"Balmaseda","year":"2006","journal-title":"Am J Trop Med Hyg"},{"year":"2019","author":"BRASIL","key":"2022100209511191600_bib25"},{"issue":"1","key":"2022100209511191600_bib26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-018-2883-8","article-title":"A high-risk zika and dengue transmission hub: virus detections in mosquitoes at a Brazilian university campus","volume":"11","author":"Eiras","year":"2018","journal-title":"Parasit Vectors"},{"issue":"1","key":"2022100209511191600_bib27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/emi.2017.59","article-title":"Zika virus replication in the mosquito culex quinquefasciatus in Brazil","volume":"6","author":"Guedes","year":"2017","journal-title":"Emerg Microbes Infect"},{"issue":"6","key":"2022100209511191600_bib28","doi-asserted-by":"crossref","first-page":"e0005654","DOI":"10.1371\/journal.pntd.0005654","article-title":"Mosquito co-infection with zika and chikungunya virus allows simultaneous transmission without affecting vector competence of Aedes aegypti","volume":"11","author":"G\u00f6ertz","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"issue":"4","key":"2022100209511191600_bib29","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1038\/s41577-019-0123-x","article-title":"Adaptive immune responses to primary and secondary dengue virus infections","volume":"19","author":"St. John","year":"2019","journal-title":"Nat Rev Immunol"},{"key":"2022100209511191600_bib30","first-page":"113","article-title":"Aedes aegypti: hist\u00f3rico do controle no Brasil","volume":"16","author":"Braga","year":"2007","journal-title":"Epidemiol Serv Sa\u00fade"}],"container-title":["Transactions of The Royal Society of Tropical Medicine and Hygiene"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/trstmh\/article-pdf\/116\/10\/924\/46287222\/trac009.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/trstmh\/article-pdf\/116\/10\/924\/46287222\/trac009.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,2]],"date-time":"2022-10-02T10:00:56Z","timestamp":1664704856000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/trstmh\/article\/116\/10\/924\/6527312"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,10]]},"references-count":30,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,2,10]]},"published-print":{"date-parts":[[2022,10,2]]}},"URL":"https:\/\/doi.org\/10.1093\/trstmh\/trac009","relation":{},"ISSN":["0035-9203","1878-3503"],"issn-type":[{"type":"print","value":"0035-9203"},{"type":"electronic","value":"1878-3503"}],"subject":[],"published-other":{"date-parts":[[2022,10]]},"published":{"date-parts":[[2022,2,10]]}}}