{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T03:16:19Z","timestamp":1771643779601,"version":"3.50.1"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T00:00:00Z","timestamp":1649203200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100000683","name":"Royal Society of Tropical Medicine and Hygiene","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000683","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001160","name":"Australian Society for Parasitology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001160","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In Cameroon, considerable research has been conducted on human ascariasis, but no studies have been undertaken to determine whether pigs contribute to the persistence of the infection in children or to unravel the evolutionary relationship between human-derived and pig-derived Ascaris.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>DNA was extracted from adult Ascaris worms collected from humans and pigs. Segments of the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes were sequenced and analysed for 83 worms to dissect the local transmission dynamics of Ascaris in Cameroon.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The data showed high genetic diversity and revealed demographically expanding populations in the human and pig Ascaris samples. A restricted gene flow between Ascaris lumbricoides and Ascaris suum populations correlating with the preference for humans and pigs, respectively, as hosts was evident. Phylogenetic analyses and haplotype networks split the haplotypes into two major clusters, A and B. However, support for cross-transmission between hosts and hybridization were revealed through shared haplotypes among worms from both hosts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study provides useful baseline information for future studies of the genetics of Ascaris in Cameroon and suggests that effective and sustainable control of human ascariasis should target both human and pig hosts.<\/jats:p><\/jats:sec>","DOI":"10.1093\/trstmh\/trac020","type":"journal-article","created":{"date-parts":[[2022,3,11]],"date-time":"2022-03-11T12:17:28Z","timestamp":1647001048000},"page":"949-958","source":"Crossref","is-referenced-by-count":1,"title":["Molecular epidemiology of<i>Ascaris<\/i>species recovered from humans and pigs in Cameroon"],"prefix":"10.1093","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6281-1541","authenticated-orcid":false,"given":"Vanessa R","family":"Nkouayep","sequence":"first","affiliation":[{"name":"Research Unit of Biology and Applied Ecology, Department of Animal Biology, Faculty of Science, University of Dschang , P.O. Box 067, Dschang, Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald P","family":"McManus","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute , Brisbane, QLD, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mpoame","family":"Mbida","sequence":"additional","affiliation":[{"name":"Research Unit of Biology and Applied Ecology, Department of Animal Biology, Faculty of Science, University of Dschang , P.O. Box 067, Dschang, Cameroon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine A","family":"Gordon","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute , Brisbane, QLD, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Nejsum","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, Faculty of Health, Aarhus University , Palle Juul-Jensens Boulevard 99, 8200 Aarhus N, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,4,6]]},"reference":[{"issue":"10100","key":"2022100209511205700_bib1","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1016\/S0140-6736(17)32154-2","article-title":"Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries, 1990\u20132016: a systematic analysis for the Global Burden of Disease Study 2016","volume":"390","author":"GBD 2016 Disease and Injury Incidence and Prevalence Collaborators","year":"2017","journal-title":"Lancet"},{"issue":"1","key":"2022100209511205700_bib2","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.gene.2011.10.043","article-title":"Comparative analyses of the complete mitochondrial genomes of Ascaris lumbricoides and Ascaris suum from humans and pigs","volume":"492","author":"Liu","year":"2012","journal-title":"Gene"},{"key":"2022100209511205700_bib3","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.meegid.2016.12.003","article-title":"Ascaris phylogeny based on multiple whole mtDNA genomes","volume":"48","author":"Nejsum","year":"2017","journal-title":"Infect Genet Evol."},{"issue":"3","key":"2022100209511205700_bib4","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/0020-7519(73)90109-4","article-title":"Value of the specific distinction between Ascaris lumbrico\u00efdes Linn\u00e9 1758 and Ascaris suum Goeze 1782","volume":"3","author":"Ansel","year":"1973","journal-title":"Int J Parasitol."},{"key":"2022100209511205700_bib5","article-title":"Soil-transmitted helminth infections","author":"World Health Organization"},{"key":"2022100209511205700_bib6","article-title":"Unit\u00e9 de Coordination Nationale de Lutte contre les Maladies Tropicales N\u00e9glig\u00e9es, Cameroun","author":"MINSANTE. 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