{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:05:43Z","timestamp":1776989143626,"version":"3.51.4"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2022,4,23]],"date-time":"2022-04-23T00:00:00Z","timestamp":1650672000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100004366","name":"Tabriz University of Medical Sciences","doi-asserted-by":"publisher","award":["95\/2-6\/2"],"award-info":[{"award-number":["95\/2-6\/2"]}],"id":[{"id":"10.13039\/501100004366","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005448","name":"HRM","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005448","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The occurrence of granulomatous amoebic encephalitis (GAE) was investigated due to the exposure of a large number of immunocompromised patients to opportunistic Acanthamoeba infections, which in most cases are fatal.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>In this case\u2013control study, 160 samples from the nasal mucosa of immunocompromised patients were collected between February 2019 to February 2020 in Isfahan, central Iran, using sterile cotton swabs; 150 ethnically matched controls were included. The pathogenic potential of the identified isolates was evaluated using temperature and osmotolerance assays. The identification of Acanthamoeba infection was confirmed by both morphological and phylomolecular tools.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Of 310 collected samples, 32 strains, including 25 (15.6%) and 7 (4.6%) isolates, were positive for the Acanthamoeba genus in the patient and control groups, respectively. The topology of the phylogenetic tree indicated that all the Acanthamoeba strains belonged to the T4 genotype. Only five of the isolates genotyped as T4 were positive for potential pathogenic assays. The heterogeneity analysis of 18S ribosomal RNA sequences of Acanthamoeba in human immunodeficiency virus (HIV)\/acquired immunodeficiency syndrome (AIDS) and hepatitis B and C patients revealed significant genetic diversity (haplotype diversity [Hd] 0.511) compared with that of healthy individuals (Hd 0.210).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The circulation of pathogenic isolates of Acanthamoeba, particularly in HIV\/AIDS patients, along with their genetic traits, indicates that clinicians should be more aware of fatal cases of GAE, especially in suspected encephalitis, in Iran and worldwide.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/trstmh\/trac026","type":"journal-article","created":{"date-parts":[[2022,3,18]],"date-time":"2022-03-18T20:13:00Z","timestamp":1647634380000},"page":"845-852","source":"Crossref","is-referenced-by-count":3,"title":["Occurrence and genetic evaluation of potentially pathogenic <i>Acanthamoeba<\/i> genotypes in nasal mucosa of immunocompromised patients: a case\u2013control study in Iran"],"prefix":"10.1093","volume":"116","author":[{"given":"Hamid Reza","family":"Moslemzadeh","sequence":"first","affiliation":[{"name":"Central Building of Tabriz University of Medical Sciences, Golgasht Street , Tabriz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmoud","family":"Mahami-Oskouei","sequence":"additional","affiliation":[{"name":"Central Building of Tabriz University of Medical Sciences, Golgasht Street , Tabriz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ehsan","family":"Ahmadpour","sequence":"additional","affiliation":[{"name":"Central Building of Tabriz University of Medical Sciences, Golgasht Street , Tabriz, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Niyyati","sequence":"additional","affiliation":[{"name":"Yaman 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