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Produced from raw milk, it may carry zoonotic pathogens such as Brucella. This study included a randomized survey for the prevalence of Brucella-positive MAC and its associated factors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>MAC samples (n=55), each one from a different rural family-based cheese-processing agroindustry, were analysed for Brucella by direct polymerase chain reaction (PCR) species-specific DNA detection and cultivation-based approaches.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among 55 MACs that were analysed, we found 17 Brucella DNA-positive samples (30.9% [95% confidence interval {CI} 18.7 to 43.1]) by PCR and, for the first time, from one MAC (1.8% [95% CI 0.5 to 9.7]), viable Brucella abortus was recovered by cultivation. Higher values for two variables, the number of lactating cows per herd (p=0.043) and daily milk production per herd (p=0.043), were each associated with Brucella-positive MAC, which concentrated in three high-risk and one low-risk spatial clusters.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>MAC may be a source of Brucella for humans, since the positive samples were from batches that were sold by cheesemakers. This should be of concern and encourage cooperation between the health and agriculture sectors in order to mitigate this public health risk through One Health integrated approaches.<\/jats:p><\/jats:sec>","DOI":"10.1093\/trstmh\/trac083","type":"journal-article","created":{"date-parts":[[2022,9,3]],"date-time":"2022-09-03T02:28:41Z","timestamp":1662172121000},"page":"1091-1099","source":"Crossref","is-referenced-by-count":6,"title":["Recovery of<i>Brucella<\/i>in raw milk Minas artisanal cheese approved for consumption by official inspection agency in Brazil: assessment of prevalence and risk factors through One Health integrated approaches"],"prefix":"10.1093","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0755-4415","authenticated-orcid":false,"given":"Marcio Roberto","family":"Silva","sequence":"first","affiliation":[{"name":"Embrapa Dairy Cattle , 36038-330 Juiz de Fora, Minas Gerais , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 Almeida Santos","family":"Duch","sequence":"additional","affiliation":[{"name":"Minas Gerais Agriculture and Livestock Institute , Belo Horizonte, Minas Gerais , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00f4mulo Tadeu Pace de Assis","family":"Lage","sequence":"additional","affiliation":[{"name":"Minas Gerais Agriculture and Livestock Institute , Belo Horizonte, Minas Gerais , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia Scafutto","family":"de Faria","sequence":"additional","affiliation":[{"name":"Embrapa Dairy Cattle , 36038-330 Juiz de Fora, Minas Gerais , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliane Denize Miranda","family":"Menezes","sequence":"additional","affiliation":[{"name":"Minas Gerais Agriculture and Livestock Institute , Belo Horizonte, Minas Gerais , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Batista","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Embrapa Dairy Cattle , 36038-330 Juiz de Fora, Minas Gerais , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilherme Nunes","family":"de Souza","sequence":"additional","affiliation":[{"name":"Embrapa Dairy Cattle , 36038-330 Juiz de Fora, Minas Gerais , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo Martins Soares","family":"Filho","sequence":"additional","affiliation":[{"name":"Brazilian Ministry of Agriculture, Livestock and Food Supply , 33600-000 Pedro Leopoldo, Minas Gerais , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingred Sales","family":"Preis","sequence":"additional","affiliation":[{"name":"Brazilian Ministry of Agriculture, Livestock and Food Supply , 33600-000 Pedro Leopoldo, Minas Gerais , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9rica Bravo","family":"Sales","sequence":"additional","affiliation":[{"name":"Brazilian Ministry of Agriculture, Livestock and Food Supply , 33600-000 Pedro Leopoldo, Minas Gerais , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia Gomes","family":"de Souza","sequence":"additional","affiliation":[{"name":"Brazilian Ministry of Agriculture, Livestock and Food Supply , 33600-000 Pedro Leopoldo, Minas Gerais , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1bio Ribeiro","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Embrapa Beef Cattle , 79106-550 Campo Grande , Mato Grosso do Sul, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Jos\u00e9 de Paula Souza e","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Evandro Chagas Institute , 67030-000 Ananindeua , Par\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tulio","family":"Mendes","sequence":"additional","affiliation":[{"name":"Marinha do Brasil , 20725090, Rio de Janeiro , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Pettan-Brewer","sequence":"additional","affiliation":[{"name":"School of Medicine 98195 University of Washington , Seattle , WA , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ant\u00f4nio Augusto","family":"Fonseca-J\u00fanior","sequence":"additional","affiliation":[{"name":"Brazilian Ministry of Agriculture, Livestock and Food Supply , 33600-000 Pedro Leopoldo, Minas Gerais , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,9,2]]},"reference":[{"key":"2022110111181613300_bib1","author":"Pires MCS","year":"2013","journal-title":"Mem\u00f3ria e arte do queijo do Serro: o saber sobre a mesa"},{"key":"2022110111181613300_bib2","first-page":"8","article-title":"Queijo minas artesanal: tradi\u00e7\u00e3o e qualidade que revelam minas","volume":"22","author":"Emater - 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