{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T13:38:19Z","timestamp":1771594699361,"version":"3.50.1"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2023,6,19]],"date-time":"2023-06-19T00:00:00Z","timestamp":1687132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U19 AI129392-01"],"award-info":[{"award-number":["U19 AI129392-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U19 AI089686"],"award-info":[{"award-number":["U19 AI089686"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"PNG Institute of Medical Research Training Office Masters Scholarship"},{"name":"Australian National Health and Medical Research Council Research Fellowship","award":["GNT1161627"],"award-info":[{"award-number":["GNT1161627"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Plasmodium falciparum pigment-containing leucocytes (PCLs) are associated with adverse clinical manifestations of severe malaria in African children. However, limited data exist on the association of PCLs in settings outside of Africa.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Thin films on peripheral blood slides obtained from children ages 6\u00a0months\u201310\u00a0y with severe malaria were examined for PCLs. The intraleucocytic pigment data were correlated with clinical phenotypic data such as severe anaemia, metabolic acidosis and coma to determine the association of PCLs with clinical phenotypes of severe malaria and outcome.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Of the 169 children with severe P. falciparum malaria confirmed by microscopy, 76% (129\/169) had PCLs. Compared with children without PCLs, the presence (adjusted odds ratio [AOR] 3.2 [95% confidence interval {CI} 1.5 to 6.9], p\u22640.01) and quantity (AOR 1.0 [95% CI 1.0 to 1.1], p=0.04) of pigment-containing monocytes (PCMs) was significantly associated with severe anaemia, while the quantity of both PCMs (AOR 1.0 [95% CI 1.0 to 1.1], p\u22640.01) and pigment-containing neutrophils (AOR 1.0 [95% CI 1.0 to 1.1], p=0.01) was significantly associated with metabolic acidosis. Plasma P. falciparum histidine-rich protein-2 level negatively correlated with the platelet count (r=\u22120.5, p\u22640.01) in patients with PCLs and no PCLs.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>In Papua New Guinean children with severe P. falciparum malaria, the presence and quantity of PCLs are predictors of disease severity, severe anaemia and metabolic acidosis.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/trstmh\/trad037","type":"journal-article","created":{"date-parts":[[2023,6,19]],"date-time":"2023-06-19T09:35:25Z","timestamp":1687167325000},"page":"797-803","source":"Crossref","is-referenced-by-count":2,"title":["The association of intraleucocytic malaria pigment and disease severity in Papua New Guinean children with severe <i>P. falciparum<\/i> malaria"],"prefix":"10.1093","volume":"117","author":[{"given":"Elvin","family":"Lufele","sequence":"first","affiliation":[{"name":"Vector Borne Diseases Unit, Papua New Guinea Institute of Medical Research , Madang , Papua New Guinea"},{"name":"Global Tropical Health Division, Menzies School of Health Research, Charles Darwin University , Darwin, NT, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurens","family":"Manning","sequence":"additional","affiliation":[{"name":"School of Medicine and Pharmacology, University of Western Australia , Perth , WA, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lina","family":"Lorry","sequence":"additional","affiliation":[{"name":"Vector Borne Diseases Unit, Papua New Guinea Institute of Medical Research , Madang , Papua New Guinea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan","family":"Warrel","sequence":"additional","affiliation":[{"name":"Vector Borne Diseases Unit, Papua New Guinea Institute of Medical Research , Madang , Papua New Guinea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Aipit","sequence":"additional","affiliation":[{"name":"Vector Borne Diseases Unit, Papua New Guinea Institute of Medical Research , Madang , Papua New Guinea"},{"name":"Paediatrics Division, Modilon Hospital , Madang , Papua New Guinea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leanne J","family":"Robinson","sequence":"additional","affiliation":[{"name":"Vector Borne Diseases Unit, Papua New Guinea Institute of Medical Research , Madang , Papua New Guinea"},{"name":"Vector Borne Diseases and Tropical Public Health Division, Burnet Institute , Melbourne , VIC, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0964-805X","authenticated-orcid":false,"given":"Moses","family":"Laman","sequence":"additional","affiliation":[{"name":"Vector Borne Diseases Unit, Papua New Guinea Institute of Medical Research , Madang , Papua New Guinea"},{"name":"Paediatrics Division, Modilon Hospital , Madang , Papua New Guinea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,6,19]]},"reference":[{"issue":"Suppl 1","key":"2023110722204200400_bib1","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1016\/S0035-9203(00)90300-6","article-title":"Severe falciparum malaria. 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