{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T22:19:03Z","timestamp":1793312343630,"version":"4.5.0"},"reference-count":8,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T00:00:00Z","timestamp":1705276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["6\/9-7[151]2017-ECD II"],"award-info":[{"award-number":["6\/9-7[151]2017-ECD II"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology, Government of India","doi-asserted-by":"publisher","award":["SR\/FST\/LS1-663\/2016"],"award-info":[{"award-number":["SR\/FST\/LS1-663\/2016"]}],"id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008789","name":"Department of Science and Technology, Government of West Bengal","doi-asserted-by":"publisher","award":["ST\/P\/S&T\/9G-22\/2016"],"award-info":[{"award-number":["ST\/P\/S&T\/9G-22\/2016"]}],"id":[{"id":"10.13039\/501100008789","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science Engineering and Research Board","award":["JCB\/2019\/000043"],"award-info":[{"award-number":["JCB\/2019\/000043"]}]},{"DOI":"10.13039\/501100001409","name":"DST","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Focused efforts of the visceral leishmaniasis elimination program have led to a drastic decline in cases, and the present challenge is disease monitoring, which this study aimed to assess.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>A Leishmania kinetoplastid-targeted qPCR quantified parasite load at disease presentation, and following treatment completion (n=49); an additional 80 cases were monitored after completion of treatment.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The parasite load at disease presentation was 13\u2009461.00 (2560.00\u201337764.00)\/\u00b5g gDNA, which upon completion of treatment reduced in 47 of 49 cases to 1(1\u20131)\/\u00b5g gDNA, p&amp;lt;0.0001. In 80 cases that presented &amp;gt;2 months post-treatment, their parasite burden similarly decreased to 1(1\u20131)\/\u00b5g gDNA except in 6 of 80 cases, which were qPCR positive.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>In 129 cases of visceral leishmaniasis, qPCR by quantification of parasite burden proved effective for monitoring treatment.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/trstmh\/trad103","type":"journal-article","created":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T09:22:42Z","timestamp":1705310562000},"page":"343-345","source":"Crossref","is-referenced-by-count":5,"title":["Molecular monitoring of treatment efficacy in human visceral leishmaniasis"],"prefix":"10.1093","volume":"118","author":[{"given":"Sutopa","family":"Roy","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Institute of Postgraduate Medical Education and Research , Kolkata 700020 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srija","family":"Moulik","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Institute of Postgraduate Medical Education and Research , Kolkata 700020 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surya Jyati","family":"Chaudhuri","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sarat Chandra Chattopadhyay Government Medical College and Hospital , Uluberia, Howrah 711316 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manab K","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, School of Tropical Medicine , Kolkata700073 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R P","family":"Goswami","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, School of Tropical Medicine , Kolkata700073 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bibhuti","family":"Saha","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine, School of Tropical Medicine , Kolkata700073 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5116-7298","authenticated-orcid":false,"given":"Mitali","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Institute of Postgraduate Medical Education and Research , Kolkata 700020 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,1,15]]},"reference":[{"issue":"4","key":"2024050112193095100_bib1","doi-asserted-by":"crossref","first-page":"e00048","DOI":"10.1128\/CMR.00048-18","article-title":"Recent development of visceral leishmaniasis treatments: Successes, pitfalls, and perspectives","volume":"31","author":"Alves","year":"2018","journal-title":"Clin Microbiol Rev"},{"issue":"3","key":"2024050112193095100_bib2","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1093\/cid\/cix808","article-title":"Monitoring of parasite kinetics in Indian post-kala-azar dermal leishmaniasis","volume":"66","author":"Moulik","year":"2018","journal-title":"Clin Infect Dis"},{"issue":"3","key":"2024050112193095100_bib3","doi-asserted-by":"crossref","first-page":"670","DOI":"10.3390\/diagnostics12030670","article-title":"Diagnosis of visceral leishmaniasis in an elimination setting: A validation study of the diagnostic algorithm in India","volume":"12","author":"Cloots","year":"2022","journal-title":"Diagnostics (Basel)"},{"issue":"4","key":"2024050112193095100_bib4","doi-asserted-by":"crossref","first-page":"795","DOI":"10.4269\/ajtmh.19-0179","article-title":"Effectiveness of single-dose liposomal amphotericin B in visceral leishmaniasis in Bihar","volume":"101","author":"Sundar","year":"2019","journal-title":"Am J Trop Med Hyg"},{"issue":"8","key":"2024050112193095100_bib5","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1093\/jac\/dkr185","article-title":"Study of parasite kinetics with antileishmanial drugs using real-time quantitative PCR in Indian visceral leishmaniasis","volume":"66","author":"Sudarshan","year":"2011","journal-title":"J Antimicrob Chemother"},{"issue":"5","key":"2024050112193095100_bib6","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.4269\/ajtmh.17-0094","article-title":"Efficacy and safety of liposomal amphotericin B for visceral leishmaniasis in children and adolescents at a tertiary care center in Bihar, India","volume":"97","author":"Pandey","year":"2017","journal-title":"Am J Trop Med Hyg"},{"issue":"5","key":"2024050112193095100_bib7","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1093\/cid\/ciab124","article-title":"Blood parasite load as an early marker to predict treatment response in visceral leishmaniasis in Eastern Africa","volume":"73","author":"Verrest","year":"2021","journal-title":"Clin Infect Dis"},{"key":"2024050112193095100_bib8","doi-asserted-by":"crossref","first-page":"e0456","DOI":"10.1590\/0037-8682-0456-2022","article-title":"Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: A case series study","volume":"56","author":"Aquino","year":"2023","journal-title":"Rev Soc Bras Med Trop"}],"container-title":["Transactions of The Royal Society of Tropical Medicine and 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