{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:31:06Z","timestamp":1759336266625,"version":"3.37.3"},"reference-count":6,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T00:00:00Z","timestamp":1712534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Melioidosis is caused by Burkholderia pseudomallei. Direct molecular detection from unamplified blood remains insensitive.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Three different extraction methods\u2014QIAamp UCP Pathogen Mini Kit, High Pure PCR template and MagNA Pure Pathogen Universal\u2014were trialled using spiked human ethylenediaminetetraacetic acid blood. A type III secretion system 1 (TTSS-1) polymerase chain reaction was used for detection.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The QIAamp UCP Pathogen Mini Kit performed best, with a limit of detection of 1.5\u00d7102\u00a0cfu\/ml.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>It is planned to use the QIAamp UCP Pathogen Mini Kit to do a larger study on blood collected from patients with melioidosis.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/trstmh\/trae020","type":"journal-article","created":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T09:13:24Z","timestamp":1712567604000},"page":"629-631","source":"Crossref","is-referenced-by-count":1,"title":["A comparison of DNA extraction methods used in the direct molecular detection of <i>Burkholderia pseudomallei<\/i> from blood"],"prefix":"10.1093","volume":"118","author":[{"given":"Georgia","family":"Mataira","sequence":"first","affiliation":[{"name":"Pathology Queensland, Townsville University Hospital , Douglas, Townsville, Queensland 4814 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fleur","family":"Francis","sequence":"additional","affiliation":[{"name":"Pathology Queensland, Townsville University Hospital , Douglas, Townsville, Queensland 4814 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaimie","family":"Frazer","sequence":"additional","affiliation":[{"name":"Pathology Queensland, Townsville University Hospital , Douglas, Townsville, Queensland 4814 , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3948-9272","authenticated-orcid":false,"given":"Robert","family":"Norton","sequence":"additional","affiliation":[{"name":"Pathology Queensland, Townsville University Hospital , Douglas, Townsville, Queensland 4814 , Australia"},{"name":"Faculty of Medicine, University of Queensland , Brisbane , Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,4,8]]},"reference":[{"issue":"2","key":"2024090212560116200_bib1","doi-asserted-by":"crossref","first-page":"e00006","DOI":"10.1128\/CMR.00006-19","article-title":"Human melioidosis","volume":"33","author":"Gassiep","year":"2020","journal-title":"Clin Microbiol Rev"},{"issue":"8","key":"2024090212560116200_bib2","doi-asserted-by":"crossref","first-page":"e12485","DOI":"10.1371\/journal.pone.0012485","article-title":"Defining the true sensitivity of culture for the diagnosis of melioidosis using Bayesian latent class models","volume":"5","author":"Limmathurotsakul","year":"2010","journal-title":"PLoS 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Rep"},{"issue":"8","key":"2024090212560116200_bib6","doi-asserted-by":"crossref","first-page":"3028","DOI":"10.1128\/JCM.00913-06","article-title":"Clinical evaluation of a type III secretion system real-time PCR assay for diagnosing melioidosis","volume":"44","author":"Meumann","year":"2006","journal-title":"J Clin Microbiol"}],"container-title":["Transactions of The Royal Society of Tropical Medicine and 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