{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T20:30:11Z","timestamp":1788294611374,"version":"build-2803163510"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,2,8]],"date-time":"2025-02-08T00:00:00Z","timestamp":1738972800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000974","name":"Australian Centre for International Agricultural Research","doi-asserted-by":"publisher","award":["LS\/2014\/055"],"award-info":[{"award-number":["LS\/2014\/055"]}],"id":[{"id":"10.13039\/501100000974","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Faecal microscopy is the mainstay of soil-transmitted helminth diagnosis and commonly completed on formalin-fixed samples when resources are insufficient to analyse fresh samples. This study assessed the diagnostic sensitivity of microscopic techniques using formalin-fixed samples.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Formalin-fixed faecal samples from 574 individuals were tested by the formalin-ethyl acetate concentration technique (FECT), Malachite smear, McMaster and McMaster2 methods. Agreement between tests was assessed by Kappa. Bayesian latent class models and a composite reference standard estimated the diagnostic sensitivity of each test.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Moderate-to-good agreement between tests was observed for A. lumbricoides. Agreement was poorer for hookworm and Trichuris trichiura. The FECT (72.70%, credible interval [CrI]: 68.92\u201376.56%) and McMaster2 method (67.93%, 95% CrIs: 62.41\u201373.31%) had the highest sensitivities for A. lumbricoides. For hookworm, the McMaster2 method (70.56%, 95% CrIs: 64.10\u201376.96%) was more sensitive than all other tests. For T. trichiura, the McMaster (90.10%, 95% CrIs: 83.29\u201394.67%) and McMaster2 (89.3%, 95% CrIs: 82.28\u201394.52%) methods were the most sensitive.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The McMaster2 method is a viable alternative to FECT and provides important information on the intensity of infection. The effect of formalin-fixation on test performance may not be as great as previously assumed. This study reports formalin-fixed sensitivities similar to previous estimates using fresh samples.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/trstmh\/traf011","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T04:16:53Z","timestamp":1737433013000},"page":"596-605","source":"Crossref","is-referenced-by-count":3,"title":["Diagnostic sensitivity of formalin-fixed faecal microscopy for the detection of soil-transmitted helminths"],"prefix":"10.1093","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2741-1059","authenticated-orcid":false,"given":"Andrew","family":"Larkins","sequence":"first","affiliation":[{"name":"Molecular and Forensic Sciences, Murdoch University School of Medical, , 90 South St, Perth 6150 ,","place":["Australia"]},{"name":"Harry Butler Institute, Murdoch University Centre for Biosecurity and One Health, , 90 South St, Perth 6150 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