{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,5]],"date-time":"2025-06-05T12:40:05Z","timestamp":1749127205879,"version":"3.41.0"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T00:00:00Z","timestamp":1739404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Consejo Nacional de Ciencia y T\u00e9cnica"},{"name":"Agencia Nacional de Promoci\u00f3n de la Ciencia y la Tecnolog\u00eda"},{"name":"Fundaci\u00f3n Argentina de Nanotecnolog\u00eda"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Leishmaniasis still imposes a heavy burden on many health systems and remains a public health problem. Early diagnosis in dogs, which act as a major reservoir for the pathogen, is central. The intention of epidemiologic studies is to identify the disease early, allowing rapid intervention to reduce its effects. This study aimed to develop two types of diagnostics tools, an enzyme-linked immunosorbent assay (ELISA) and a lateral flow immunochromatography assay for recognizing canine visceral leishmaniasis (CVL).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Multiwell ELISA plates were sensitized and strips were designed with the same chimeric recombinant antigen from Leishmania spp. (LeQuiDi) and the results were compared. The sera panel contained 409 samples, including 244 positives and 165 negatives. Of the 165 negative sera, 67 were obtained from Corrientes city, an endemic area for CVL in Argentina, and 98 of this group from La Plata city, a non-endemic area in Argentina (they were seropositive for other infections, as specificity control).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Serum with an elevated concentration of lipids, or bilirubin, or a variable percentage of haemolysis were tested without interference. Both the sensitivity and specificity of the LeQuiDi-based tests were high: \u226593% for ELISA and \u226587% for LFIA.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our study introduces LeQuiDi as a promising antigen for CVL diagnosis, regardless of the test format.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/trstmh\/traf012","type":"journal-article","created":{"date-parts":[[2025,1,20]],"date-time":"2025-01-20T20:18:00Z","timestamp":1737404280000},"page":"606-612","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of suitability of two tools for the diagnosis of canine visceral leishmaniasis"],"prefix":"10.1093","volume":"119","author":[{"given":"Valeria S","family":"Garcia","sequence":"first","affiliation":[{"name":"Grupo de Pol\u00edmeros y Reactores de Polimerizaci\u00f3n-Instituto de Desarrollo Tecnol\u00f3gico para la Industria Qu\u00edmica (UNL-CONICET) , G\u00fcemes 3450, 3000 - Santa Fe ,","place":["Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maira S","family":"Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Universidad Nacional del Litoral C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, , Paraje El Pozo, Ciudad Universitaria, 3000 - Santa Fe ,","place":["Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ver\u00f3nica D G","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Grupo de Pol\u00edmeros y Reactores de Polimerizaci\u00f3n-Instituto de Desarrollo Tecnol\u00f3gico para la Industria Qu\u00edmica (UNL-CONICET) , G\u00fcemes 3450, 3000 - Santa Fe ,","place":["Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis M","family":"Gugliotta","sequence":"additional","affiliation":[{"name":"Grupo de Pol\u00edmeros y Reactores de Polimerizaci\u00f3n-Instituto de Desarrollo Tecnol\u00f3gico para la Industria Qu\u00edmica (UNL-CONICET) , G\u00fcemes 3450, 3000 - Santa Fe ,","place":["Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexis","family":"Burna","sequence":"additional","affiliation":[{"name":"Universidad Nacional del Nordeste Catedra de Patolog\u00eda General y Sistem\u00e1tica Facultad de Ciencias Veterinarias, , Sargento Cabral 2139, W3402BKG - Corrientes ,","place":["Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego","family":"Eiras","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunoparasitologia Facultad de Ciencias Veterinarias Universidad Nacional de La Plata , Calle 60 y 118, 1900 - La Plata ,","place":["Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mat\u00edas S","family":"Cabeza","sequence":"additional","affiliation":[{"name":"Universidad Nacional del Litoral C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, , Paraje El Pozo, Ciudad Universitaria, 3000 - Santa Fe ,","place":["Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego G","family":"Arias","sequence":"additional","affiliation":[{"name":"Laboratorio de Enzimolog\u00eda Molecular - Instituto de Agrobiotecnolog\u00eda del Litoral (UNL-CONICET) , Colectora Ruta Nac. 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