{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T14:43:07Z","timestamp":1780497787535,"version":"3.54.1"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2025,4,12]],"date-time":"2025-04-12T00:00:00Z","timestamp":1744416000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100000683","name":"Royal Society of Tropical Medicine and Hygiene","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000683","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004441","name":"Swedish International Development Cooperation Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004441","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Penn Center for AIDS Research","award":["5-P30-AI-045008-17"],"award-info":[{"award-number":["5-P30-AI-045008-17"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The emergence of Plasmodium falciparum resistance to artemisinin is a huge concern in Africa. We investigated and characterized mutations of Pfk13 propeller sequences from P. falciparum isolates across two endemic areas with different eco-geographical settings in Cameroon.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>A total of 259 dried blood spot samples were collected through a cross-sectional survey in two health facilities located in Bimengue (rural area) and the Efoulan District Hospital in Yaound\u00e9 (urban area). The molecular analysis of Pfk13 was performed using nested polymerase chain reaction followed by Sanger sequencing.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Our findings highlight that of the 23 mutations found, most of the genetic variants were observed in Yaound\u00e9, suggesting a possible higher drug pressure in this locality. Three Pfk13 mutations (F446S, C469W and A681D) were found in polymorphic sites known to be associated with artemisinin resistance, but with different amino acid substitutions. In addition, three other mutations have already been reported to circulate in other African countries, with an unknown impact on delayed response to artemisinin therapy (P475L, S477F and F628L).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Although there is an absence of validated artemisinin resistance\u2013associated polymorphisms in the study, the results highlight the need for further studies to quantify the frequency of these and other Pfk13 gene polymorphisms over time.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/trstmh\/traf040","type":"journal-article","created":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T17:31:06Z","timestamp":1743269466000},"page":"1030-1040","source":"Crossref","is-referenced-by-count":4,"title":["Investigating <i>Pfk13<\/i> mutations in <i>Plasmodium falciparum<\/i> natural populations from two malaria-endemic areas of Cameroon"],"prefix":"10.1093","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-7537-2837","authenticated-orcid":false,"given":"Charlotte S","family":"Milong Melong","sequence":"first","affiliation":[{"name":"University of Douala Department of Biochemistry, , Douala ,","place":["Cameroon"]},{"name":"Botswana\u2013University of Pennsylvania Partnership , Gaborone ,","place":["Botswana"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4286-147X","authenticated-orcid":false,"given":"Loick P","family":"Kojom Foko","sequence":"additional","affiliation":[{"name":"University of Douala Department of Animal Organisms, Faculty of Science, , Douala ,","place":["Cameroon"]},{"name":"Center for Expertise and Research in Applied Biology , Douala ,","place":["Cameroon"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4292-8440","authenticated-orcid":false,"given":"Francis 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