{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:36:30Z","timestamp":1792794990814,"version":"4.5.0"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T00:00:00Z","timestamp":1747699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Office of Infectious Diseases, Bureau for Global Health","id":[{"id":"https:\/\/ror.org\/01z6p8p31","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Malaria Vaccine Development Program","award":["OAA-C-15-00071"],"award-info":[{"award-number":["OAA-C-15-00071"]}]},{"DOI":"10.13039\/501100000272","name":"National Institute for Health Research","doi-asserted-by":"publisher","award":["CL-2016-04-502"],"award-info":[{"award-number":["CL-2016-04-502"]}],"id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]},{"name":"UK Department of Health and Social Care","id":[{"id":"https:\/\/ror.org\/03sbpja79","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Wellcome Trust Research Training Fellowship","award":["108734\/Z\/15\/Z"],"award-info":[{"award-number":["108734\/Z\/15\/Z"]}],"id":[{"id":"https:\/\/ror.org\/029chgv08","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Wellcome Trust Senior Fellowship","award":["106917\/Z\/15\/Z"],"award-info":[{"award-number":["106917\/Z\/15\/Z"]}],"id":[{"id":"https:\/\/ror.org\/029chgv08","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Malaria is the most common tropical infection in the UK. Current guidelines suggest that testing on 3 consecutive days is required following an initial negative result. This study aimed to see whether newer diagnostics (loop-mediated amplification assay [LAMP]) had sufficient sensitivity to support a change in diagnostic practice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Blood samples from 11 participants who had undergone controlled human malaria infection (CHMI) with Plasmodium falciparum malaria were assessed from day 6 (C+6) for malaria positivity using the Carestart Malaria rapid diagnostic test (RDT) and from C+4 using the Alethia Malaria LAMP assay. Quantitative polymerase chain reaction had been performed twice daily during CHMI follow-up. A retrospective analysis of samples submitted to the Sheffield Teaching Hospitals for malaria testing over a 5-y period was conducted, evaluating the combination of the Carestart RDT alongside blood film analysis, as per UK guidelines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In CHMI samples, LAMP was positive for all parasitaemias &amp;gt;1000\u00a0parasites\/ml, whereas RDTs were less reliable (59% positive for parasitaemias &amp;gt;1000\u00a0parasites\/ml). The combination of RDT and blood films for clinical samples diagnosed most infections, but only a minority of negative samples had subsequent tests.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>LAMP has higher sensitivity than current UK recommended methods, with a potential to review the requirement for additional days of testing in the majority of patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/trstmh\/traf050","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T07:44:38Z","timestamp":1745394278000},"page":"1149-1156","source":"Crossref","is-referenced-by-count":3,"title":["Diagnosis of\n                    <i>Plasmodium falciparum<\/i>\n                    malaria at very low parasitaemias using a commercially available LAMP assay and RDT"],"prefix":"10.1093","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8490-671X","authenticated-orcid":false,"given":"Ruth O","family":"Payne","sequence":"first","affiliation":[{"name":"University of Oxford The Jenner Institute, , Old Road Campus Research Building, Roosevelt Drive, Oxford OX3\u00a07DQ ,","place":["UK"]},{"name":"University of Sheffield Division of Clinical Medicine, School of Medicine and Population Health, , Sheffield S10\u00a02RX ,","place":["UK"]},{"name":"Northern General Hospital Laboratory Medicine, Sheffield Teaching Hospitals NHS Foundation Trust, , Herries Road, Sheffield, S5 7AU ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nick J","family":"Edwards","sequence":"additional","affiliation":[{"name":"University of Oxford The Jenner Institute, , Old Road Campus Research Building, Roosevelt Drive, Oxford OX3\u00a07DQ 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