{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:30:10Z","timestamp":1759336210200,"version":"build-2065373602"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T00:00:00Z","timestamp":1748476800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100012537","name":"Ditmanson Medical Foundation Chia-Yi Christian Hospital","doi-asserted-by":"publisher","award":["R104-017"],"award-info":[{"award-number":["R104-017"]}],"id":[{"id":"10.13039\/501100012537","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Summer Medical Student Research Program 2014"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Anisakis simplex is a parasitic zoonosis associated with consuming raw or undercooked fish. Despite its global relevance, data from Africa are scarce. This study assessed the seroprevalence and risk factors of A. simplex infection among primary schoolchildren (PSC) in Makoko, an urban slum in Lagos, Nigeria.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>A cross-sectional study was conducted from November 2013 to March 2014 involving 196 PSC from three government schools. Serodiagnosis was performed using enzyme-linked immunosorbent assay (ELISA) with recombinant Ani s 1 and Ani s 7 allergens. Stool samples were examined for soil-transmitted helminths (STHs) using the Kato\u2013Katz method. Structured questionnaires gathered demographic and dietary information.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The overall seroprevalence of A. simplex infection was 12.25%. No significant association was found with gender or raw fish consumption. However, parental education and occupation influenced seropositivity. Interestingly, co-infection with STHs was inversely associated with A. simplex seropositivity. ELISA demonstrated high specificity with minimal cross-reactivity to STHs.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>This study provides the first seroepidemiological data on A. simplex in Nigerian children. Public health interventions, particularly fish-handling education, are warranted. Further research is needed to understand the clinical relevance of A. simplex sensitization in African settings.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/trstmh\/traf055","type":"journal-article","created":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T09:17:40Z","timestamp":1748510260000},"page":"1184-1191","source":"Crossref","is-referenced-by-count":0,"title":["Seroprevalence and risk factors of <i>Anisakis simplex<\/i> in Makoko schoolchildren: insights from a Lagos, Nigeria slum"],"prefix":"10.1093","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5579-8169","authenticated-orcid":false,"given":"Chia-Kwung","family":"Fan","sequence":"first","affiliation":[{"name":"School of Medicine, College of Medicine, Taipei Medical University Department of Molecular Parasitology and Tropical Diseases, , 250 Wu-Xing Street, Xing-Yi District, 110301 Taipei ,","place":["Taiwan"]},{"name":"Taipei Medical University Research Center of International Tropical Medicine, College of Medicine, , 250 Wu-Xing Street, Xing-Yi District, 110301 Taipei ,","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting-Wu","family":"Chuang","sequence":"additional","affiliation":[{"name":"School of Medicine, College of Medicine, Taipei Medical University Department of Molecular Parasitology and Tropical Diseases, , 250 Wu-Xing Street, Xing-Yi District, 110301 Taipei ,","place":["Taiwan"]},{"name":"Taipei Medical University Research Center of International Tropical Medicine, College of Medicine, , 250 Wu-Xing Street, Xing-Yi District, 110301 Taipei ,","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hon-Ian","family":"Lei","sequence":"additional","affiliation":[{"name":"School of Medicine, College of Medicine, Taipei Medical University Department of Molecular Parasitology and Tropical Diseases, , 250 Wu-Xing Street, Xing-Yi District, 110301 Taipei ,","place":["Taiwan"]},{"name":"Taipei Medical University Research Center of International Tropical Medicine, College of Medicine, , 250 Wu-Xing Street, Xing-Yi District, 110301 Taipei ,","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent P","family":"Gyang","sequence":"additional","affiliation":[{"name":"Nigerian Institute of Medical Research Public Health Division, Molecular Parasitology Research Laboratory, , 6, Edmund Crescent off Murtala Mohammed Way, P.M.B. 2013 Yaba, Lagos ,","place":["Nigeria"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tyng-Shiuan","family":"Hsieh","sequence":"additional","affiliation":[{"name":"College of Medicine and College of Engineering, National Taiwan University Department of Biomedical Engineering, , No. 1, Sec. 4, Roosevelt Rd., 10617 Taipei ,","place":["Taiwan"]},{"name":"Cancer Branch Department of Surgery and Section of Dermatology,"},{"name":"National Taiwan University Hospital Department of Dermatology, , No. 1, Sec. 1, Jen\u2013Ai Rd., Taipei 100 ,","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Po-Ching","family":"Cheng","sequence":"additional","affiliation":[{"name":"School of Medicine, College of Medicine, Taipei Medical University Department of Molecular Parasitology and Tropical Diseases, , 250 Wu-Xing Street, Xing-Yi District, 110301 Taipei ,","place":["Taiwan"]},{"name":"Taipei Medical University Research Center of International Tropical Medicine, College of Medicine, , 250 Wu-Xing Street, Xing-Yi District, 110301 Taipei ,","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chia-Mei","family":"Chou","sequence":"additional","affiliation":[{"name":"School of Medicine, College of Medicine, Taipei Medical University Department of Molecular Parasitology and Tropical Diseases, , 250 Wu-Xing Street, Xing-Yi District, 110301 Taipei ,","place":["Taiwan"]},{"name":"Taipei Medical University Research Center of International Tropical Medicine, College of Medicine, , 250 Wu-Xing Street, Xing-Yi District, 110301 Taipei ,","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olaoluwa P","family":"Akinwale","sequence":"additional","affiliation":[{"name":"Nigerian Institute of Medical Research Public Health Division, Molecular Parasitology Research Laboratory, , 6, Edmund Crescent off Murtala Mohammed Way, P.M.B. 2013 Yaba, Lagos ,","place":["Nigeria"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,5,29]]},"reference":[{"issue":"8","key":"2025100108461374300_bib1","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1007\/s00436-023-07881-9","article-title":"A critical review of anisakidosis cases occurring globally","volume":"122","author":"Shamsi","year":"2023","journal-title":"Parasitol 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