{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T08:14:39Z","timestamp":1758960879090,"version":"3.37.3"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2018,3,15]],"date-time":"2018-03-15T00:00:00Z","timestamp":1521072000000},"content-version":"vor","delay-in-days":73,"URL":"https:\/\/academic.oup.com\/journals\/pages\/about_us\/legal\/notices"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fundac\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul"},{"DOI":"10.13039\/501100002322","name":"Coordenac\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"name":"PROPESQ\/UFRGS"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,1,1]]},"DOI":"10.1093\/trstmh\/try017","type":"journal-article","created":{"date-parts":[[2018,2,8]],"date-time":"2018-02-08T15:11:47Z","timestamp":1518102707000},"page":"14-21","source":"Crossref","is-referenced-by-count":15,"title":["Backyard pigs are a reservoir of zoonotic hepatitis E virus in southern Brazil"],"prefix":"10.1093","volume":"112","author":[{"given":"M S","family":"da Silva","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Virologia, Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gon\u00e7alves 9090, CEP 91-540-000, Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Silveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia, Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gon\u00e7alves 9090, CEP 91-540-000, Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V S","family":"Caron","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia, Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gon\u00e7alves 9090, CEP 91-540-000, Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A C S","family":"M\u00f3sena","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia, Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gon\u00e7alves 9090, CEP 91-540-000, Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M N","family":"Weber","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia, Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gon\u00e7alves 9090, CEP 91-540-000, Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S P","family":"Cibulski","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia, Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gon\u00e7alves 9090, CEP 91-540-000, Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A A R","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Epidemiologia, Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G S","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Epidemiologia, Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L G","family":"Corbellini","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Epidemiologia, Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Klein","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia e Imunologia Avan\u00e7ada, Faculdade de Agronomia e Medicina Veterin\u00e1ria, Universidade de Passo Fundo (UPF), Passo Fundo, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L C","family":"Kreutz","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia e Imunologia Avan\u00e7ada, Faculdade de Agronomia e Medicina Veterin\u00e1ria, Universidade de Passo Fundo (UPF), Passo Fundo, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Frandoloso","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Microbiologia e Imunologia Avan\u00e7ada, Faculdade de Agronomia e Medicina Veterin\u00e1ria, Universidade de Passo Fundo (UPF), Passo Fundo, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C W","family":"Canal","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Virologia, Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gon\u00e7alves 9090, CEP 91-540-000, Porto Alegre, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2018,3,2]]},"reference":[{"key":"key\n\t\t\t\t20180329100320_try017C1","doi-asserted-by":"crossref","first-page":"2223","DOI":"10.1099\/vir.0.068429-0","article-title":"Consensus proposals for classification of the family Hepeviridae","volume":"95(Pt 5)","author":"Smith","year":"2014","journal-title":"J Gen Virol"},{"key":"key\n\t\t\t\t20180329100320_try017C2","first-page":"1","article-title":"Zoonotic hepatitis E virus: Classification, animal reservoirs and transmission routes","volume":"8(10)","author":"Doceul","year":"2016","journal-title":"Viruses"},{"key":"key\n\t\t\t\t20180329100320_try017C3","doi-asserted-by":"crossref","first-page":"2477","DOI":"10.1016\/S0140-6736(11)61849-7","article-title":"Hepatitis E","volume":"379(9835)","author":"Kamar","year":"2012","journal-title":"Lancet"},{"year":"2012","author":"IBGE","key":"key\n\t\t\t\t20180329100320_try017C4"},{"key":"key\n\t\t\t\t20180329100320_try017C5","first-page":"1","article-title":"Panorama da suinocultura no Rio Grande do Sul","volume":"33","author":"Poeta","year":"2014","journal-title":"Dep Def e Agropecu\u00e1ria"},{"key":"key\n\t\t\t\t20180329100320_try017C6","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0166-0934(99)00052-X","article-title":"Rapid detection of hepatitis E virus RNA by reverse transcription-polymerase chain reaction using universal oligonucleotide primers","volume":"81(1\u20132)","author":"Erker","year":"1999","journal-title":"J Virol Methods"},{"key":"key\n\t\t\t\t20180329100320_try017C7","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.trstmh.2007.03.014","article-title":"Hepatitis E: a neglected threat","volume":"102(3)","author":"Myint","year":"2008","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"key\n\t\t\t\t20180329100320_try017C8","first-page":"1","article-title":"Extra-hepatic manifestations associated with hepatitis E virus infection\u202f: a comprehensive review of the literature","volume":"4(1)","author":"Bazerbachi","year":"2016","journal-title":"Gastroenterol Rep (Oxf)"},{"key":"key\n\t\t\t\t20180329100320_try017C9","first-page":"4425","article-title":"Chronic hepatitis E virus infection in a pediatric female liver transplant recipient","volume":"52(12)","author":"Guimar\u00e3es","year":"2014","journal-title":"J Clin Microbiol"},{"key":"key\n\t\t\t\t20180329100320_try017C10","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1002\/jmv.23494","article-title":"First report and molecular characterization of hepatitis E virus infection in renal transplant recipients in Brazil","volume":"85(4)","author":"Passos","year":"2013","journal-title":"J Med Virol"},{"key":"key\n\t\t\t\t20180329100320_try017C11","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s12917-015-0322-z","article-title":"Seroprevalence and molecular characteristics of hepatitis E virus in household-raised pig population in the Philippines","volume":"11","author":"Liu","year":"2015","journal-title":"BMC Vet Res"},{"key":"key\n\t\t\t\t20180329100320_try017C12","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1590\/S0074-02762005000200003","article-title":"Serological evidence of hepatitis E virus infection in different animal species from the southeast of Brazil","volume":"100(2)","author":"Vitral","year":"2005","journal-title":"Mem Inst Oswaldo Cruz Rio Janeiro"},{"key":"key\n\t\t\t\t20180329100320_try017C13","doi-asserted-by":"crossref","first-page":"e0176409","DOI":"10.1371\/journal.pone.0176409","article-title":"In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG anti-hepatitis E virus amongst blood donors in south Brazil","volume":"12(5)","author":"Pandolfi","year":"2017","journal-title":"PLoS One"},{"key":"key\n\t\t\t\t20180329100320_try017C14","doi-asserted-by":"crossref","first-page":"fnw021","DOI":"10.1093\/femsle\/fnw021","article-title":"Production and characterization of a Brazilian candidate antigen for hepatitis E virus genotype 3 diagnosis","volume":"363(5)","author":"de Almeida Ramos","year":"2016","journal-title":"FEMS Microbiol Lett"},{"key":"key\n\t\t\t\t20180329100320_try017C15","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.virusres.2013.01.005","article-title":"Genetic heterogeneity and subtyping of human hepatitis E virus isolates from Uruguay","volume":"173(2)","author":"Mirazo","year":"2013","journal-title":"Virus Res"},{"key":"key\n\t\t\t\t20180329100320_try017C16","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1128\/JCM.40.4.1326-1332.2002","article-title":"Detection by reverse transcription-PCR and genetic characterization of field isolates of swine hepatitis E virus from pigs in different geographic regions of the United States","volume":"40(4)","author":"Huang","year":"2002","journal-title":"J Clin Microbiol"},{"key":"key\n\t\t\t\t20180329100320_try017C17","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1093\/bioinformatics\/bts199","article-title":"Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data","volume":"28(12)","author":"Kearse","year":"2012","journal-title":"Bioinformatics"},{"key":"key\n\t\t\t\t20180329100320_try017C18","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets","volume":"33(7)","author":"Kumar","year":"2016","journal-title":"Mol Biol Evol"},{"key":"key\n\t\t\t\t20180329100320_try017C19","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1099\/jgv.0.000393","article-title":"Proposed reference sequences for hepatitis E virus subtypes","volume":"97(3)","author":"Smith","year":"2016","journal-title":"J Gen Virol"},{"key":"key\n\t\t\t\t20180329100320_try017C20","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1590\/1678-4162-7733","article-title":"Molecular detection of hepatitis E virus in feces and slurry from swine farms, Rio Grande do Sul, southern Brazil","volume":"67(3)","author":"Vasconcelos","year":"2015","journal-title":"Arq Bras Med Veterin\u00e1ria e Zootec"},{"key":"key\n\t\t\t\t20180329100320_try017C21","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1590\/S0074-02762012000700016","article-title":"Hepatitis E virus in liver and bile samples from slaughtered pigs of Brazil","volume":"107(7)","author":"Gardinali","year":"2012","journal-title":"Mem Inst Oswaldo Cruz"},{"volume-title":"Veterinary epidemiology","year":"2007","author":"Thrusfield","key":"key\n\t\t\t\t20180329100320_try017C22"},{"key":"key\n\t\t\t\t20180329100320_try017C23","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.jcv.2009.12.021","article-title":"First report of a human autochthonous hepatitis E virus infection in Brazil","volume":"47(3)","author":"Lopes Dos Santos","year":"2010","journal-title":"J Clin Virol"},{"key":"key\n\t\t\t\t20180329100320_try017C24","first-page":"1807","article-title":"Correlation between positivity for immunoglobulin A antibodies and viraemia of swine hepatitis E virus observed among farm pigs in Japan","volume":"86(Pt 6)","author":"Takahashi","year":"2017","journal-title":"J Gen Virol"},{"key":"key\n\t\t\t\t20180329100320_try017C25","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.vetmic.2013.10.001","article-title":"Age-related and regional differences in the prevalence of hepatitis E virus-specific antibodies in pigs in Germany","volume":"167(3\u20134)","author":"Krumbholz","year":"2013","journal-title":"Vet Microbiol"},{"key":"key\n\t\t\t\t20180329100320_try017C26","first-page":"1","article-title":"Prevalence of hepatitis E virus antibodies in pigs in northern Italy","volume":"1","author":"Martinelli","year":"2011","journal-title":"Infect Ecol Epidemiol"},{"key":"key\n\t\t\t\t20180329100320_try017C27","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1016\/j.jcv.2013.08.030","article-title":"Hepatitis E virus seroprevalence and risk factors for individuals in working contact with animals","volume":"58(3)","author":"Chaussade","year":"2013","journal-title":"J Clin Virol"},{"key":"key\n\t\t\t\t20180329100320_try017C28","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1007\/s12560-016-9243-7","article-title":"Hepatitis E virus in surface water, sediments, and pork products marketed in southern Brazil","volume":"8(3)","author":"Heldt","year":"2016","journal-title":"Food Environ Virol"},{"key":"key\n\t\t\t\t20180329100320_try017C29","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1746-6148-5-7","article-title":"The course of hepatitis E virus infection in pigs after contact-infection and intravenous inoculation","volume":"5(1)","author":"Bouwknegt","year":"2009","journal-title":"BMC Vet Res"},{"key":"key\n\t\t\t\t20180329100320_try017C30","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.bjm.2016.10.022","article-title":"High frequency of hepatitis E virus infection in swine from south Brazil and close similarity to human HEV isolates","volume":"48(2)","author":"Passos-Castilho","year":"2017","journal-title":"Braz J Microbiol"}],"container-title":["Transactions of The Royal Society of Tropical Medicine and Hygiene"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/trstmh\/article-pdf\/112\/1\/14\/24510572\/try017.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,3,29]],"date-time":"2018-03-29T10:03:36Z","timestamp":1522317816000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/trstmh\/article\/112\/1\/14\/4938534"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,1]]},"references-count":30,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2018,3,2]]},"published-print":{"date-parts":[[2018,1,1]]}},"URL":"https:\/\/doi.org\/10.1093\/trstmh\/try017","relation":{},"ISSN":["0035-9203","1878-3503"],"issn-type":[{"type":"print","value":"0035-9203"},{"type":"electronic","value":"1878-3503"}],"subject":[],"published-other":{"date-parts":[[2018,1]]},"published":{"date-parts":[[2018,1,1]]}}}