{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T18:22:03Z","timestamp":1784830923545,"version":"3.55.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2019,10,14]],"date-time":"2019-10-14T00:00:00Z","timestamp":1571011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Yaws is a neglected tropical disease and results in lesions of skin, soft tissues and bones. PCR plays an important part in surveillance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Children suspected to have yaws were enrolled. From the largest lesion, paired swabs were collected, one in transport medium and one as a dry swab. In children with multiple lesions we collected additional swabs from up to four subsequent lesions. Swabs in transport medium were maintained in a cold chain while dry swabs were stored at ambient temperature. Swabs were tested by PCR for Treponema pallidum and Haemophilus ducreyi.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Of 55 individuals, 10 (18%) had at least one positive PCR for T. pallidum and 12 (22%) had at least one positive result for H. ducreyi. Concordance was 100% between swabs in transport medium and dry swabs. One patient had PCR-confirmed yaws on the swab of a third lesion when both the first and second lesions were PCR-negative.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Storing swabs in transport medium and transporting in a cold chain did not improve yield, however, detection of T. pallidum is increased by swabbing additional lesions. As the target for yaws is eradication, approaches to sample collection need revisiting to ensure cases are not missed.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/trstmh\/trz083","type":"journal-article","created":{"date-parts":[[2019,7,24]],"date-time":"2019-07-24T07:24:18Z","timestamp":1563953058000},"page":"776-780","source":"Crossref","is-referenced-by-count":4,"title":["Optimising the use of molecular tools for the diagnosis of yaws"],"prefix":"10.1093","volume":"113","author":[{"given":"Morgan","family":"Munson","sequence":"first","affiliation":[{"name":"Clinical Research Department, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Creswell","sequence":"additional","affiliation":[{"name":"Clinical Research Department, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kofi","family":"Kondobala","sequence":"additional","affiliation":[{"name":"Ghana Health Service, Accra, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bawa","family":"Ganiwu","sequence":"additional","affiliation":[{"name":"Ghana Health Service, Accra, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita Dede","family":"Lomotey","sequence":"additional","affiliation":[{"name":"Ghana Health Service, Accra, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Oppong","sequence":"additional","affiliation":[{"name":"Ghana Health Service, Accra, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederick Opoku","family":"Agyeman","sequence":"additional","affiliation":[{"name":"Ghana Health Service, Accra, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nana","family":"Kotye","sequence":"additional","affiliation":[{"name":"Ghana Health Service, Accra, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mukaila","family":"Diwura","sequence":"additional","affiliation":[{"name":"Ghana Health Service, Accra, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebenezer Padi","family":"Ako","sequence":"additional","affiliation":[{"name":"Ghana Health Service, Accra, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shirley Victoria","family":"Simpson","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kennedy Kwasi","family":"Addo","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry","family":"Pickering","sequence":"additional","affiliation":[{"name":"Clinical Research Department, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Becca L","family":"Handley","sequence":"additional","affiliation":[{"name":"Clinical Research Department, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Houghton","sequence":"additional","affiliation":[{"name":"Clinical Research Department, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Kwakye","sequence":"additional","affiliation":[{"name":"Ghana Health Service, Accra, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Marks","sequence":"additional","affiliation":[{"name":"Clinical Research Department, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London, UK"},{"name":"Hospital for Tropical Diseases, Mortimer Market Centre, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,10,14]]},"reference":[{"key":"2019121207373188500_ref1","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1093\/trstmh\/tru128","article-title":"Endemic treponemal diseases","volume":"108","author":"Marks","year":"2014","journal-title":"Trans R Soc Trop Med 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