{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T01:32:31Z","timestamp":1772155951376,"version":"3.50.1"},"reference-count":62,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2022,8,24]],"date-time":"2022-08-24T00:00:00Z","timestamp":1661299200000},"content-version":"vor","delay-in-days":54,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P31503-B26"],"award-info":[{"award-number":["P31503-B26"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,12,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mixed human cytomegalovirus (HCMV) strain infections are frequent in lung transplant recipients (LTRs). To date, the influence of the donor (D) and recipient (R) HCMV serostatus on intra-host HCMV strain composition and viral population dynamics after transplantation is only poorly understood. Here, we investigated ten pre-transplant lungs from HCMV-seropositive donors and 163 sequential HCMV-DNA-positive plasma and bronchoalveolar lavage samples from fifty LTRs with multiviremic episodes post-transplantation. The study cohort included D+R+ (38\u2009per cent), D+R\u2212 (36\u2009per cent), and D\u2212R+ (26\u2009per cent) patients. All samples were subjected to quantitative genotyping by short amplicon deep sequencing, and twenty-four of them were additionally PacBio long-read sequenced for genotype linkages. We find that D+R+ patients show a significantly elevated intra-host strain diversity compared to D+R\u2212 and D\u2212R+ patients (P\u2009=\u20090.0089). Both D+ patient groups display significantly higher viral population dynamics than D\u2212 patients (P\u2009=\u20090.0061). Five out of ten pre-transplant donor lungs were HCMV DNA positive, whereof three multiple HCMV strains were detected, indicating that multi-strain transmission via lung transplantation is likely. Using long reads, we show that intra-host haplotypes can share distinctly linked genotypes, which limits overall intra-host diversity in mixed infections. Together, our findings demonstrate donor-derived strains as the main source of increased HCMV strain diversity and dynamics post-transplantation. These results foster strategies to mitigate the potential transmission of the donor strain reservoir to the allograft, such as ex vivo delivery of HCMV-selective immunotoxins prior to transplantation to reduce latent HCMV.<\/jats:p>","DOI":"10.1093\/ve\/veac076","type":"journal-article","created":{"date-parts":[[2022,8,24]],"date-time":"2022-08-24T11:46:54Z","timestamp":1661341614000},"source":"Crossref","is-referenced-by-count":8,"title":["Human cytomegalovirus strain diversity and dynamics reveal the donor lung as a major contributor after transplantation"],"prefix":"10.1093","volume":"8","author":[{"given":"B\u00fcsra","family":"K\u00fclekci","sequence":"first","affiliation":[{"name":"Center for Virology, Medical University of Vienna , Kinderspitalgasse 15, Vienna 1090, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Medical University of Vienna , W\u00e4hringer G\u00fcrtel 18-20, Vienna 1090, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadja","family":"Brait","sequence":"additional","affiliation":[{"name":"Cluster of Microbial Ecology, Groningen Institute for Evolutionary Life Sciences, University of Groningen , Nijenborgh 7, Groningen 9747 AG, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Perkmann-Nagele","sequence":"additional","affiliation":[{"name":"Division of Clinical Virology, Medical University of Vienna , W\u00e4hringer G\u00fcrtel 18-20, Vienna 1090, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Jaksch","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Medical University of Vienna , W\u00e4hringer G\u00fcrtel 18-20, Vienna 1090, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konrad","family":"Hoetzenecker","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Medical University of Vienna , W\u00e4hringer G\u00fcrtel 18-20, Vienna 1090, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"Puchhammer-St\u00f6ckl","sequence":"additional","affiliation":[{"name":"Center for Virology, Medical University of Vienna , Kinderspitalgasse 15, Vienna 1090, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Goerzer","sequence":"additional","affiliation":[{"name":"Center for Virology, Medical University of Vienna , Kinderspitalgasse 15, Vienna 1090, 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