{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:48:43Z","timestamp":1790668123358,"version":"4.1.0"},"reference-count":81,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T00:00:00Z","timestamp":1764979200000},"content-version":"vor","delay-in-days":339,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["#2023\/09610-8"],"award-info":[{"award-number":["#2023\/09610-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["307209\/2023-7"],"award-info":[{"award-number":["307209\/2023-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CAPES-Humboldt Research Fellowship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Understanding the viral diversity harboured by wildlife is essential for effective mapping and prevention of future zoonotic outbreaks. Bats, in particular, are recognized as natural reservoirs for several high-impact zoonotic viral pathogens, including coronaviruses responsible for Severe Acute Respiratory Syndrome (SARS), the rabies virus, diverse paramyxoviruses, Marburg, Ebola, Nipah, and Hendra viruses. However, a large extent of bat viruses remains unexplored, especially in highly biodiverse regions of the Neotropics such as Brazilian ecosystems. We used a meta-transcriptomic approach to characterize new virus genomes found in blood, oral, and anal samples collected from cave- and noncave bats from Northeast Brazil, Caatinga, and Atlantic Forest biomes. From a total of 19 coronavirus-positive bats, we have assembled two complete genomes of a new Betacoronavirus subgenus, named Ambecovirus (American betacoronavirus). The subgenus herein described is phylogenetically placed between the Sarbeco-\/Hibeco-\/Nobecovirus and the Merbeco-\/Embecovirus clades, being basal to the former. While the conserved S2 region of the spike protein retained hallmark domains, including HR1 and HR2, the S1\/S2 cleavage site and the furin cleavage site, the S1 region consistently displayed only the N-terminal domain. The receptor-binding domain from the C-terminal domai (CTD) region could not be identified due to high dissimilarity relative to known congeners. The detection of Ambercovirus in sympatric Pteronotus gymnonotus and Carollia perspicillata bats suggests potential interspecies transmission. Longitudinal sampling confirmed persistent Ambecovirus infection in P. gymnonotus over multiple years and virus dispersion at a minimum distance of 270\u00a0km between caves. The present study confirms that viral diversity in neotropical hosts remains largely unknown, not just in Brazil but likely in the other countries of the region, supporting the need for a systematic approach to virome exploration and analysis followed by in vitro experimentation to assess zoonotic potential.<\/jats:p>","DOI":"10.1093\/ve\/veaf094","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T13:20:07Z","timestamp":1764854407000},"source":"Crossref","is-referenced-by-count":4,"title":["<i>Ambecovirus<\/i>\n                    , a novel\n                    <i>Betacoronavirus<\/i>\n                    subgenus circulating in neotropical bats, sheds new light on bat-borne coronaviruses evolution"],"prefix":"10.1093","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1419-5713","authenticated-orcid":false,"given":"Gabriel da Luz","family":"Wallau","sequence":"first","affiliation":[{"name":"Departamento de Entomologia, Instituto Aggeu Magalh\u00e3es, Funda\u00e7\u00e3o Oswaldo Cruz , Avenida Professor Moraes Rego s\/n, Bairro Cidade Universit\u00e1ria, 50740-465, Recife, Pernambuco ,","place":["Brazil"]},{"name":"N\u00facleo de Bioinform\u00e1tica, Instituto Aggeu Magalh\u00e3es, Funda\u00e7\u00e3o Oswaldo Cruz , Avenida Professor Moraes Rego s\/n, Bairro Cidade Universit\u00e1ria, 50740-465, Recife, Pernambuco ,","place":["Brazil"]},{"name":"Department of Arbovirology and Entomology, Bernhard Nocht Institute for Tropical Medicine , Bernhard-Nocht-Strasse 74, 20359, Hamburg ,","place":["Germany"]},{"name":"Universidade Federal de Santa Maria (UFSM) , Av. Roraima n\u00ba 1000, Bairro - Camobi, 97105-900, Santa Maria, Rio Grande do Sul ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eder","family":"Barbier","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ci\u00eancia Aplicada \u00e0 Conserva\u00e7\u00e3o da Biodiversidade, Departamento de Zoologia, Centro de Bioci\u00eancias, Universidade Federal de Pernambuco UFPE , Avenida Professor Moraes Rego s\/n, Bairro Cidade Universit\u00e1ria, 50670-901, Recife, Pernambuco ,","place":["Brazil"]},{"name":"Departamento de Patologia, Reprodu\u00e7\u00e3o e Sa\u00fade \u00danica, Universidade Estadual Paulista , Via de Acesso Professor Paulo Donato Castellane, s\/n, Vila Industrial, 14884-900, Jaboticabal, S\u00e3o Paulo ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lais Ceschini","family":"Machado","sequence":"additional","affiliation":[{"name":"Departamento de Entomologia, Instituto Aggeu Magalh\u00e3es, Funda\u00e7\u00e3o Oswaldo Cruz , Avenida Professor Moraes Rego s\/n, Bairro Cidade Universit\u00e1ria, 50740-465, Recife, Pernambuco ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8034-8562","authenticated-orcid":false,"given":"Alexandre Freitas","family":"da Silva","sequence":"additional","affiliation":[{"name":"N\u00facleo de Bioinform\u00e1tica, Instituto Aggeu Magalh\u00e3es, Funda\u00e7\u00e3o Oswaldo Cruz , Avenida Professor Moraes Rego s\/n, Bairro Cidade Universit\u00e1ria, 50740-465, Recife, Pernambuco ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yago Jose Mariz","family":"Dias","sequence":"additional","affiliation":[{"name":"N\u00facleo de Bioinform\u00e1tica, Instituto Aggeu Magalh\u00e3es, Funda\u00e7\u00e3o Oswaldo Cruz , Avenida Professor Moraes Rego s\/n, Bairro Cidade Universit\u00e1ria, 50740-465, Recife, Pernambuco 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,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto D","family":"Lins","sequence":"additional","affiliation":[{"name":"Departamento de Virologia, Instituto Aggeu Magalh\u00e3es, Funda\u00e7\u00e3o Oswaldo Cruz , Avenida Professor Moraes Rego s\/n, Bairro Cidade Universit\u00e1ria, 50740-465, Recife, Pernambuco ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Bernard","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ci\u00eancia Aplicada \u00e0 Conserva\u00e7\u00e3o da Biodiversidade, Departamento de Zoologia, Centro de Bioci\u00eancias, Universidade Federal de Pernambuco UFPE , Avenida Professor Moraes Rego s\/n, Bairro Cidade Universit\u00e1ria, 50670-901, Recife, Pernambuco ,","place":["Brazil"]},{"name":"Laborat\u00f3rio de Ci\u00eancia em Biodiversidade , Departamento de Ecologia e Conserva\u00e7\u00e3o, Instituto de Ci\u00eancias Naturais, , Trevo Rotat\u00f3rio 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