{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T09:50:18Z","timestamp":1782899418535,"version":"3.54.5"},"reference-count":74,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":161,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EU Hera project DURABLE","award":["101102733"],"award-info":[{"award-number":["101102733"]}]},{"name":"German Federal Ministry of Education and Research through the German Centre for Infection Research","award":["8064701703"],"award-info":[{"award-number":["8064701703"]}]},{"name":"German Federal Ministry of Education and Research through the German Centre for Infection Research","award":["8040701710"],"award-info":[{"award-number":["8040701710"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Arboviruses evolve under unique ecological constraints imposed by their dual replication cycles in vertebrate and arthropod hosts. This dual-host requirement results in markedly low substitution rates, which complicate molecular clock calibration, particularly when temporal sampling spans only narrow time windows. For RNA arboviruses, wide sampling windows are especially rare due to the intrinsic instability of RNA. Here, we demonstrate that ethanol-preserved museum specimens can help overcome these temporal limitations. We successfully recovered the coding-complete genome of a bandavirus, a negative-sense segmented RNA virus that clusters with the highly pathogenic human severe fever with thrombocytopenia syndrome virus. The virus was detected in a Common pipistrelle (Pipistrellus pipistrellus) bat collected in northern Germany in 1919, making it one of the oldest sequenced mammalian RNA viruses, only comparable to historic measles and influenza A viruses from 1912 and 1918. Screening 1086 contemporary bat samples revealed strains of the same virus species in nine Common pipistrelle bats (2010\u20132018) and one Serotine bat (Eptesicus serotinus, 1999) from Germany and the Netherlands. Coding-complete genomes indicate frequent genome segment reassortment and widespread circulation of reassortants of this understudied virus species (Bandavirus zwieselense). We detected an exceptionally low substitution rate (&amp;lt;\u20096.88\u2009\u00d7\u200910\u22125 substitutions\/site\/year) between the RdRp coding sequence of the ancient genome and its nearest modern counterpart. Additionally, functional assays demonstrated that the virus\u2019s non-structural (NSs) protein effectively inhibits interferon induction in human HEK-293T cells. Our findings highlight the feasibility and scientific value of extracting and analysing ancient viral RNA from ethanol-preserved museum specimens to substantially enhance our understanding of RNA arbovirus evolution.<\/jats:p>","DOI":"10.1093\/ve\/veag034","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T15:33:06Z","timestamp":1781191986000},"source":"Crossref","is-referenced-by-count":0,"title":["Extending the temporal window of arbovirus evolutionary analysis through the recovery of a century-old bandavirus"],"prefix":"10.1093","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7623-9463","authenticated-orcid":false,"given":"Udo","family":"Gieraths","sequence":"first","affiliation":[{"name":"Institute of Virology, Campus Charit\u00e9 Mitte, Charit\u00e9 - Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin , Charit\u00e9pl. 1, Berlin 10117, Berlin ,","place":["Germany"]},{"name":"Faculty of Life Sciences, Humboldt Universit\u00e4t zu Berlin , Invalidenstra\u00dfe 42, Berlin 10115 ,","place":["Berlin, Germany"]},{"name":"Institute of Virology, Freiburg University Medical Center, Faculty of Medicine, University of Freiburg , Hermann Herder Stra\u00dfe 11, 79104 Freiburg, Baden-W\u00fcrttemberg ,","place":["Germany"]},{"name":"Section Clinical Virus Genomics, Institute of Virology, University Medical Center Freiburg , Hermann Herder Stra\u00dfe 11, 79104 Freiburg, Baden-W\u00fcrttemberg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rn","family":"Beheim-Schwarzbach","sequence":"additional","affiliation":[{"name":"Institute of Virology, Campus Charit\u00e9 Mitte, Charit\u00e9 - Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin , Charit\u00e9pl. 1, Berlin 10117, Berlin ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J","family":"Pickin","sequence":"additional","affiliation":[{"name":"Institute for Virology, FB10-Veterinary Medicine, Justus-Liebig University , Schubertstr. 81, 35392 Gie\u00dfen, Hessen ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2741-9904","authenticated-orcid":false,"given":"Annika","family":"Beyer","sequence":"additional","affiliation":[{"name":"Institute of Virology, Campus Charit\u00e9 Mitte, Charit\u00e9 - Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin , Charit\u00e9pl. 1, Berlin 10117, Berlin ,","place":["Germany"]},{"name":"Faculty of Life Sciences, Humboldt Universit\u00e4t zu Berlin , Invalidenstra\u00dfe 42, Berlin 10115 ,","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lineke","family":"Begeman","sequence":"additional","affiliation":[{"name":"Department of Viroscience, Erasmus University Medical Centre , Dr. Molewaterplein 40, 3015GD, Rotterdam ,","place":["the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health , S\u00fcdufer 10, Greifswald-Insel Riems 17493, Mecklenburg-Vorpommern ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3999-0393","authenticated-orcid":false,"given":"Kore","family":"Schlottau","sequence":"additional","affiliation":[{"name":"Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health , S\u00fcdufer 10, Greifswald-Insel Riems 17493, Mecklenburg-Vorpommern ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Beer","sequence":"additional","affiliation":[{"name":"Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health , S\u00fcdufer 10, Greifswald-Insel Riems 17493, Mecklenburg-Vorpommern ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5620-1528","authenticated-orcid":false,"given":"Rainer G","family":"Ulrich","sequence":"additional","affiliation":[{"name":"Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health , S\u00fcdufer 10, Greifswald-Insel Riems, Mecklenburg-Vorpommern ,","place":["Germany"]},{"name":"German Centre for Infection Research (DZIF), Partner Site Hamburg-L\u00fcbeck-Borstel-Riems , S\u00fcdufer 10, Greifswald-Insel Riems 17493, Mecklenburg-Vorpommern ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Institute for Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health , S\u00fcdufer 10, Greifswald-Insel Riems 17493, Mecklenburg-Vorpommern ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Conrad M","family":"Freuling","sequence":"additional","affiliation":[{"name":"Institute for Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health , S\u00fcdufer 10, Greifswald-Insel Riems 17493, Mecklenburg-Vorpommern ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiina","family":"Mauno","sequence":"additional","affiliation":[{"name":"Institute of Virology, Campus Charit\u00e9 Mitte, Charit\u00e9 - Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin , Charit\u00e9pl. 1, Berlin 10117, Berlin ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"van de Bildt","sequence":"additional","affiliation":[{"name":"Department of Viroscience, Erasmus University Medical Centre , Dr. Molewaterplein 40, 3015GD, Rotterdam ,","place":["the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera C","family":"Mols","sequence":"additional","affiliation":[{"name":"Department of Viroscience, Erasmus University Medical Centre , Dr. Molewaterplein 40, 3015GD, Rotterdam ,","place":["the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3605-0136","authenticated-orcid":false,"given":"Victor M","family":"Corman","sequence":"additional","affiliation":[{"name":"Institute of Virology, Campus Charit\u00e9 Mitte, Charit\u00e9 - Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin , Charit\u00e9pl. 1, Berlin 10117, Berlin ,","place":["Germany"]},{"name":"German Centre for Infection Research (DZIF), Partner Site Charit\u00e9 , Charit\u00e9pl. 1, Berlin 10117, Berlin ,","place":["Germany"]},{"name":"Labor Berlin - Charit\u00e9 Vivantes GmbH , Sylter Stra\u00dfe 2, 13353 Berlin, Berlin ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9737-337X","authenticated-orcid":false,"given":"Friedemann","family":"Weber","sequence":"additional","affiliation":[{"name":"Institute for Virology, FB10-Veterinary Medicine, Justus-Liebig University , Schubertstr. 81, 35392 Gie\u00dfen, Hessen ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1120-9531","authenticated-orcid":false,"given":"Terry 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