{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T12:03:10Z","timestamp":1781092990265,"version":"3.54.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1992,2,1]],"date-time":"1992-02-01T00:00:00Z","timestamp":696902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1992,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We have developed a sensitive, specific solid-phase immunoradiometric assay (IRMA) of parathyroid hormone-related protein (PTH-RP) with use of affinity-purified polyclonal immunoglobulins. Antibodies recognizing PTH-RP(37-74) are immobilized to a polystyrene bead to \"capture\" analytes from the sample; antibodies to epitopes within the 1-36 amino acid region of PTH-RP are labeled with 125I. This IRMA recognizes PTH-RP(1-74) and PTH-RP(1-86) equivalently, but does not detect N-terminal or C-terminal fragments of PTH-RP, intact human parathyrin (PTH), or fragments of PTH. PTH-RP is not stable in plasma at 3-5 degrees C or room temperature, but a mixture of aprotinin (500 kallikrein units\/L) and leupeptin (2.5 mg\/L) improves PTH-RP stability in blood samples. In plasma collected in the presence of these protease inhibitors from normal volunteers and patients with various disorders of calcium metabolism, PTH-RP concentrations were above normal (greater than 1.5 pmol\/L) in 91% (42 of 46) of patients with hypercalcemia associated with nonhematological malignancy. In plasma from patients with other hypercalcemic conditions (e.g., primary hyperparathyroidism, sarcoidosis, and vitamin D excess), PTH-RP was undetectable. Above-normal concentrations of PTH-RP and total calcium decreased to normal in a patient with an ovarian cyst adenocarcinoma after surgical removal of the tumor. We conclude that PTH-RP is related to and probably the causative agent of hypercalcemia in most patients with cancer, and that measurements of PTH-RP are useful in the diagnosis and management of patients with tumor-associated hypercalcemia.<\/jats:p>","DOI":"10.1093\/clinchem\/38.2.282","type":"journal-article","created":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T09:22:46Z","timestamp":1578907366000},"page":"282-288","source":"Crossref","is-referenced-by-count":76,"title":["Modified Immunoradiometric Assay of Parathyroid Hormone-Related Protein: Clinical Application in the Differential Diagnosis of Hypercalcemia"],"prefix":"10.1093","volume":"38","author":[{"given":"M R","family":"Pandian","sequence":"first","affiliation":[{"name":"Nichols Institute, 26441 Via de Anza, San Juan Capistrano, CA 92675"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig H","family":"Morgan","sequence":"first","affiliation":[{"name":"Nichols Institute, 26441 Via de Anza, San Juan Capistrano, CA 92675"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Carlton","sequence":"first","affiliation":[{"name":"Nichols Institute, 26441 Via de Anza, San Juan Capistrano, CA 92675"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gino V","family":"Segre","sequence":"first","affiliation":[{"name":"Massachusetts General Hospital, Endocrine Unit, Department of Medicine and Harvard Medical School, Boston, MA 02114"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1992,2,1]]},"container-title":["Clinical Chemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/clinchem\/article-pdf\/38\/2\/282\/32745409\/clinchem0282.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/clinchem\/article-pdf\/38\/2\/282\/32745409\/clinchem0282.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T06:25:29Z","timestamp":1583216729000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/clinchem\/article\/38\/2\/282\/5649818"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1992,2,1]]},"published-print":{"date-parts":[[1992,2,1]]}},"URL":"https:\/\/doi.org\/10.1093\/clinchem\/38.2.282","relation":{},"ISSN":["0009-9147","1530-8561"],"issn-type":[{"value":"0009-9147","type":"print"},{"value":"1530-8561","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1992,2,1]]},"published":{"date-parts":[[1992,2,1]]}}}