{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T23:56:25Z","timestamp":1770335785696,"version":"3.49.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[1998,7,1]],"date-time":"1998-07-01T00:00:00Z","timestamp":899251200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Schizosaccharomyces pombe is an excellent organism in which to study cytokinesis as it divides by medial fission using an F-actin contractile ring. To enhance our understanding of the cell division process, a large genetic screen was carried out in which 17 genetic loci essential for cytokinesis were identified, 5 of which are novel. Mutants identifying three genes, rng3+, rng4+, and rng5+, were defective in organizing an actin contractile ring. Four mutants defective in septum deposition, septum initiation defective (sid)1, sid2, sid3, and sid4, were also identified and characterized. Genetic analyses revealed that the sid mutants display strong negative interactions with the previously described septation mutants cdc7-24, cdc11-123, and cdc14-118. The rng5+, sid2+, and sid3+ genes were cloned and shown to encode Myo2p (a myosin heavy chain), a protein kinase related to budding yeast Dbf2p, and Spg1p, a GTP binding protein that is a member of the ras superfamily of GTPases, respectively. The ability of Spg1p to promote septum formation from any point in the cell cycle depends on the activity of Sid4p. In addition, we have characterized a phenotype that has not been described previously in cytokinesis mutants, namely the failure to reorganize actin patches to the medial region of the cell in preparation for septum formation.<\/jats:p>","DOI":"10.1093\/genetics\/149.3.1265","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T01:42:29Z","timestamp":1619574149000},"page":"1265-1275","source":"Crossref","is-referenced-by-count":240,"title":["Isolation and Characterization of New Fission Yeast Cytokinesis Mutants"],"prefix":"10.1093","volume":"149","author":[{"given":"Mohan K","family":"Balasubramanian","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute and Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232"},{"name":"Institute of Molecular Agrobiology, Singapore 118240"}]},{"given":"Dannel","family":"McCollum","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232"},{"name":"Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545"}]},{"given":"Louise","family":"Chang","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232"}]},{"given":"Kelvin C Y","family":"Wong","sequence":"additional","affiliation":[{"name":"Institute of Molecular Agrobiology, Singapore 118240"}]},{"given":"Naweed I","family":"Naqvi","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232"},{"name":"Institute of Molecular Agrobiology, Singapore 118240"}]},{"given":"Xiangwei","family":"He","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030"}]},{"given":"Shelley","family":"Sazer","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030"}]},{"given":"Kathleen L","family":"Gould","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232"}]}],"member":"286","published-online":{"date-parts":[[1998,7,1]]},"reference":[{"key":"2022010309445526400_R1","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1038\/360084a0","article-title":"A new tropomyosin essential for cytokinesis in the fission yeast S. pombe","volume":"360","author":"Balasubramanian","year":"1992","journal-title":"Nature"},{"key":"2022010309445526400_R2","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1083\/jcb.125.6.1289","article-title":"The Schizosaccharomyces pombecdc3+ gene encodes a profilin essential for cytokinesis","volume":"125","author":"Balasubramanian","year":"1994","journal-title":"J. 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Cell Sci."},{"key":"2022010309445526400_R11","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1083\/jcb.137.1.169","article-title":"cdc12p, a protein required for cytokinesis in fission yeast, is a component of the cell division ring and interacts with profilin","volume":"137","author":"Chang","year":"1997","journal-title":"J. Cell Biol."},{"key":"2022010309445526400_R12","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1126\/science.276.5309.118","article-title":"Bni1p, a yeast formin linking Cdc42p and the actin cytoskeleton during polarized morphogenesis","volume":"276","author":"Evangelista","year":"1997","journal-title":"Science"},{"key":"2022010309445526400_R13","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1091\/mbc.4.5.531","article-title":"The Schizosaccharomyces pombe cdc14 gene is required for septum formation and can also inhibit nuclear division","volume":"4","author":"Fankhauser","year":"1993","journal-title":"Mol. Biol. 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