{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:07:29Z","timestamp":1779343649626,"version":"3.51.4"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[1998,9,1]],"date-time":"1998-09-01T00:00:00Z","timestamp":904608000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In wild-type Saccharomyces cerevisiae, a checkpoint slows the rate of progression of an ongoing S phase in response to exposure to a DNA-alkylating agent. Mutations that eliminate S phase regulation also confer sensitivity to alkylating agents, leading us to suggest that, by regulating the S phase rate, cells are either better able to repair or better able to replicate damaged DNA. In this study, we determine the effects of mutations that impair S phase regulation on the ability of excision repair-defective cells to replicate irreparably UV-damaged DNA. We assay survival after UV irradiation, as well as the genetic consequences of replicating a damaged template, namely mutation and sister chromatid exchange induction. We find that RAD9, RAD17, RAD24, and MEC3 are required for UV-induced (although not spontaneous) mutagenesis, and that RAD9 and RAD17 (but not REV3, RAD24, and MEC3) are required for maximal induction of replication-dependent sister chromatid exchange. Therefore, checkpoint genes not only control cell cycle progression in response to damage, but also play a role in accommodating DNA damage during replication.<\/jats:p>","DOI":"10.1093\/genetics\/150.1.75","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T02:11:07Z","timestamp":1619575867000},"page":"75-93","source":"Crossref","is-referenced-by-count":108,"title":["The Saccharomyces cerevisiae RAD9, RAD17, RAD24 and MEC3 Genes Are Required for Tolerating Irreparable, Ultraviolet-Induced DNA Damage"],"prefix":"10.1093","volume":"150","author":[{"given":"A G","family":"Paulovich","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C D","family":"Armour","sequence":"additional","affiliation":[{"name":"Fred Hutchinson Cancer Research Center, Seattle, Washington 98109"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L H","family":"Hartwell","sequence":"additional","affiliation":[{"name":"Fred Hutchinson Cancer Research Center, Seattle, Washington 98109"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1998,9,1]]},"reference":[{"key":"2022010311030154300_R1","first-page":"3224","article-title":"Semidominant suppressors of Srs2 helicase mutation of Saccharomyces cerevisiae map in the RAD51 gene, whose sequence predicts a protein with similarities to prokaryotic RecA proteins","volume":"12","author":"Aboussekhra","year":"1992","journal-title":"Mol. Cell. Biol."},{"key":"2022010311030154300_R2","doi-asserted-by":"crossref","first-page":"3912","DOI":"10.1002\/j.1460-2075.1996.tb00765.x","article-title":"A novel role for the budding yeast RAD9 checkpoint gene in DNA damage-dependent transcription","volume":"15","author":"Aboussekhra","year":"1996","journal-title":"EMBO J."},{"key":"2022010311030154300_R3","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1007\/BF00418125","article-title":"Yeast arginine permease: nucleotide sequence of the CAN1 gene","volume":"10","author":"Ahmad","year":"1986","journal-title":"Curr. 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Bacteriol."},{"key":"2022010311030154300_R40","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1093\/genetics\/127.1.75","article-title":"The hypergene conversion hpr5-1 mutation of Saccharomyces cerevisiae is an allele of the SRS2\/RADH gene","volume":"127","author":"Rong","year":"1991","journal-title":"Genetics"},{"key":"2022010311030154300_R41","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/0076-6879(83)01015-0","article-title":"One-step gene disruption in yeast","volume":"101","author":"Rothstein","year":"1983","journal-title":"Methods Enzymol."},{"key":"2022010311030154300_R42","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1093\/genetics\/124.4.817","article-title":"The SRS2 suppressor of rad6 mutations of Saccharomyces cerevisiae acts by channeling DNA lesions in to the RAD52 DNA repair pathway","volume":"124","author":"Schiestl","year":"1990","journal-title":"Genetics"},{"key":"2022010311030154300_R43","volume-title":"Methods in Yeast Genetics","author":"Sherman","year":"1981"},{"key":"2022010311030154300_R44","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1007\/BF00331068","article-title":"Analysis of mutagenic DNA repair in a thermoconditional mutant of Saccharomyces cerevisiae. I. Influence of cycloheximide on UV-irradiated rev2 ts cells","volume":"190","author":"Siede","year":"1983","journal-title":"Mol. Gen. Genet."},{"key":"2022010311030154300_R45","doi-asserted-by":"crossref","first-page":"7985","DOI":"10.1073\/pnas.90.17.7985","article-title":"RAD9-dependent G1 arrest defines a second checkpoint for damaged DNA in the cell cycle of Saccharomyces cerevisiae","volume":"90","author":"Siede","year":"1993","journal-title":"Proc. Natl. Acad. Sci. 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