{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T14:18:56Z","timestamp":1786630736764,"version":"build-2736575974"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"18","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1991,9,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We demonstrate that the DNA polymerase isolated from Thermococcus litoralis (VentTM DNA polymerase) is the first thermostable DNA polymerase reported having a 3\u2032\u20145\u2032 proofreading exonuclease activity. This facilitates a highly accurate DNA synthesis in vitro by the polymerase. Mutational frequencies observed in the base substitution fidelity assays were in the range of 30\u00d710\u22126. These values were 5\u201310 times lower compared to other thermostable DNA polymerases lacking the proofreading activity. All classes of DNA polymerase errors (transitions, transversions, frameshift mutations) were assayed using the forward mutational assay (1). The mutation frequencies of Thermococcus litoralis DNA polymerase varied between 15\u221235\u00d710\u22124 being 2 \u2013 4 times lower than the respective values obtained using enzymes without proofreading activity. We also noticed that the fidelity of the DNA polymerase from Thermococcus litoralis responds to changes in dNTP concentration, units of enzyme used per one reaction and the concentration of MgSO4 relative to the total concentration of dNTPs present in the reaction. The high fidelity DNA synthesis In vitro by Thermococcus litoralis DNA polymerase provides good possibilities for maintaining the genetic information of original target DNA sequences intact in the DNA amplification applications.<\/jats:p>","DOI":"10.1093\/nar\/19.18.4967","type":"journal-article","created":{"date-parts":[[2007,1,4]],"date-time":"2007-01-04T21:00:53Z","timestamp":1167944453000},"page":"4967-4973","source":"Crossref","is-referenced-by-count":183,"title":["Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase\u2014an extremely heat stable enzyme with proofreading activity"],"prefix":"10.1093","volume":"19","author":[{"given":"P.","family":"Mattila","sequence":"first","affiliation":[{"name":"Finnzymes Oy Riihitontuntie 14 B, PO Box 148, SF 02200 Espoo, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Korpela","sequence":"additional","affiliation":[{"name":"Finnzymes Oy Riihitontuntie 14 B, PO Box 148, SF 02200 Espoo, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Tenkanen","sequence":"additional","affiliation":[{"name":"Finnzymes Oy Riihitontuntie 14 B, PO Box 148, SF 02200 Espoo, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Pitk\u00e4mem","sequence":"additional","affiliation":[{"name":"Finnzymes Oy Riihitontuntie 14 B, PO Box 148, SF 02200 Espoo, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1991,9,25]]},"container-title":["Nucleic Acids Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/nar\/article-pdf\/19\/18\/4967\/30095535\/19-18-4967.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T09:51:56Z","timestamp":1570009916000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/nar\/article\/19\/18\/4967\/1078626"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,9,25]]},"references-count":0,"journal-issue":{"issue":"18","published-online":{"date-parts":[[1991,9,25]]},"published-print":{"date-parts":[[1991,9,25]]}},"URL":"https:\/\/doi.org\/10.1093\/nar\/19.18.4967","relation":{},"ISSN":["1362-4962","0305-1048"],"issn-type":[{"value":"1362-4962","type":"electronic"},{"value":"0305-1048","type":"print"}],"subject":[],"published-other":{"date-parts":[[1991,9,25]]},"published":{"date-parts":[[1991,9,25]]}}}