{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:36:21Z","timestamp":1773966981158,"version":"3.50.1"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2015,7,8]],"date-time":"2015-07-08T00:00:00Z","timestamp":1436313600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,3,1]]},"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n Recent studies have shown that preeclampsia (PE) is associated with the presence of autoantibodies (AABs) that activate the angiotensin II AT 1 receptor, which could contribute to many of the symptoms of PE.<\/jats:p>\n <\/jats:sec>\n \n METHODS<\/jats:title>\n To investigate the frequency and the targets of AABs in preeclamptic women (31 cases) and healthy pregnant normotensive women (29 cases) in Brazil, antibodies from serum samples were detected by a bioassay using spontaneously beating neonatal rat cardiomyocytes in culture. In the cardiomyocytes, the agonistic AABs induce a positive or negative chronotropic response, mimicking the corresponding receptor agonists. The specificity of the AAB response was identified by specific receptor antagonists.<\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n Thirty preeclamptic patients (97%) presented AABs against the angiotensin II AT 1 receptor. The agonistic effect of the AAB was blocked by irbesartan and neutralized by a peptide corresponding to the second extracellular loop of this receptor. Strikingly, we discovered that all sera from the severe preeclamptic patients (16 cases) contained a novel agonist-like AAB directed against the endothelin-1 ETA receptor in addition to the AABs against the angiotensin II AT 1 receptor. This AAB was selectively blocked by the antagonist BQ-123, antagonized by the protein kinase C (PKC) inhibitor Calphostin C and neutralized by peptides corresponding to the second extracellular loop of the endothelin-1 ETA receptor subtype.<\/jats:p>\n <\/jats:sec>\n \n CONCLUSIONS<\/jats:title>\n We described, for the first time, the presence of endothelin-1 ETA receptor AABs in PE. Our results suggest that the presence of both agonistic AABs may be involved in the pathogenesis of severe PE.<\/jats:p>\n <\/jats:sec>","DOI":"10.1093\/ajh\/hpv099","type":"journal-article","created":{"date-parts":[[2015,7,10]],"date-time":"2015-07-10T01:19:27Z","timestamp":1436491167000},"page":"405-412","source":"Crossref","is-referenced-by-count":21,"title":["Identification of a Novel Agonist-Like Autoantibody in Preeclamptic\n Patients"],"prefix":"10.1093","volume":"29","author":[{"given":"Elizabeth Portugal","family":"Velloso","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, Federal University of Minas Gerais, Belo Horizonte 1 , Minas Gerais , Brazil ;"}]},{"given":"Renata L\u00facia","family":"Pimentel","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Federal University of Minas Gerais, Belo Horizonte 1 , Minas Gerais , Brazil ;"}]},{"given":"Jana\u00edna F.","family":"Braga","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Federal University of Minas Gerais, Belo Horizonte 1 , Minas Gerais , Brazil ;"}]},{"given":"Ant\u00f4nio Carlos Vieira","family":"Cabral","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, Federal University of Minas Gerais, Belo Horizonte 2 , Minas Gerais , Brazil ;"}]},{"given":"Zilma Silveira N.","family":"Reis","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, Federal University of Minas Gerais, Belo Horizonte 2 , Minas Gerais , Brazil ;"}]},{"given":"Michael","family":"Bader","sequence":"additional","affiliation":[{"name":"Max-Delbr\u00fcck-Center for Molecular Medicine (MDC) 3 , Berlin-Buch , Germany ."}]},{"given":"Robson Augusto S.","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Federal University of Minas Gerais, Belo Horizonte 1 , Minas Gerais , Brazil ;"}]},{"given":"Gerd","family":"Wallukat","sequence":"additional","affiliation":[{"name":"Max-Delbr\u00fcck-Center for Molecular Medicine (MDC) 3 , Berlin-Buch , Germany ."}]}],"member":"286","published-online":{"date-parts":[[2015,7,8]]},"reference":[{"key":"2024012721430374200_CIT0001","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1081\/PRG-120016792","article-title":"Summary of the NHLBI Working Group on Research on\n Hypertension During Pregnancy","volume":"22","author":"Roberts","year":"2003","journal-title":"Hypertens\n Pregnancy"},{"key":"2024012721430374200_CIT0002","first-page":"1122","article-title":"Hypertension in\n pregnancy. 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