{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:59:21Z","timestamp":1791244761578,"version":"4.1.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Soluble support (tag)-assisted liquid-phase peptide synthesis (LPPS) is a greener alternative to solid-phase peptide synthesis (SPPS) because it reduces the consumption of reagents and solvents throughout the synthetic process. In conventional LPPS, tagged peptides are selectively precipitated and isolated by filtration; however, filtration can be time-consuming depending on the physical properties of the precipitate. Herein, we report a magnetic decantation-enabled, filtration-free LPPS protocol using chemically stable and reversibly dispersible hydrophobic magnetic nanoparticles. Magnetic decantation performed after each coupling\/deprotection sequence enables pot- and time-economical peptide synthesis without filtration. The feasibility of the protocol was demonstrated through the synthesis of leucine-enkephalin as a model peptide, affording the target peptide in excellent yield and high purity without chromatographic purification.<\/jats:p>","DOI":"10.1093\/bbb\/zbag149","type":"journal-article","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T11:42:05Z","timestamp":1790941325000},"source":"Crossref","is-referenced-by-count":0,"title":["Magnetic decantation-enabled pot- and time-economical liquid-phase peptide synthesis"],"prefix":"10.1093","author":[{"given":"Jun","family":"Karasawa","sequence":"first","affiliation":[{"name":"Tokyo University of Agriculture and Technology Department of Applied Biological Science, , 3-5-8 Sawai-cho, Fuchu, Tokyo, 183-8509 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Sudo","sequence":"additional","affiliation":[{"name":"Tokyo University of Agriculture and Technology Department of Applied Biological Science, , 3-5-8 Sawai-cho, Fuchu, Tokyo, 183-8509 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shohei","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Tokyo University of Agriculture and Technology Department of Applied Biological Science, , 3-5-8 Sawai-cho, Fuchu, Tokyo, 183-8509 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshikazu","family":"Kitano","sequence":"additional","affiliation":[{"name":"Tokyo University of Agriculture and Technology Department of Applied Biological Science, , 3-5-8 Sawai-cho, Fuchu, Tokyo, 183-8509 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4353-1595","authenticated-orcid":false,"given":"Yohei","family":"Okada","sequence":"additional","affiliation":[{"name":"Tokyo University of Agriculture and Technology Department of Applied Biological Science, , 3-5-8 Sawai-cho, Fuchu, Tokyo, 183-8509 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,5]]},"container-title":["Bioscience, Biotechnology, and Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bbb\/advance-article-pdf\/doi\/10.1093\/bbb\/zbag149\/71606404\/zbag149.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bbb\/advance-article-pdf\/doi\/10.1093\/bbb\/zbag149\/71606404\/zbag149.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:41:22Z","timestamp":1791243682000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bbb\/advance-article\/doi\/10.1093\/bbb\/zbag149\/8865314"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,5]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/bbb\/zbag149","relation":{},"ISSN":["1347-6947"],"issn-type":[{"value":"1347-6947","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,5]]},"article-number":"zbag149"}}