{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T15:07:32Z","timestamp":1775920052712,"version":"3.50.1"},"reference-count":100,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2021,3,23]],"date-time":"2021-03-23T00:00:00Z","timestamp":1616457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Portuguese Foundation for Science and Technology","award":["SFRH\/BD\/137935\/2018"],"award-info":[{"award-number":["SFRH\/BD\/137935\/2018"]}]},{"name":"Portuguese Foundation for Science and Technology","award":["EF (CEECIND\/00627\/2017)"],"award-info":[{"award-number":["EF (CEECIND\/00627\/2017)"]}]},{"name":"Portuguese Foundation for Science and Technology","award":["MLL (2020.03608.CEECIND)"],"award-info":[{"award-number":["MLL (2020.03608.CEECIND)"]}]},{"name":"Portugal 2020 and the European Union through the ERDF, and by FCT through national funds"},{"DOI":"10.13039\/100006129","name":"FCT","doi-asserted-by":"publisher","award":["UIDB\/04423\/2020"],"award-info":[{"award-number":["UIDB\/04423\/2020"]}],"id":[{"id":"10.13039\/100006129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006129","name":"FCT","doi-asserted-by":"publisher","award":["UIDP\/04423\/2020"],"award-info":[{"award-number":["UIDP\/04423\/2020"]}],"id":[{"id":"10.13039\/100006129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,5,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Since historical times, the inherent human fascination with pearls turned the freshwater pearl mussel Margaritifera margaritifera (Linnaeus, 1758) into a highly valuable cultural and economic resource. Although pearl harvesting in M. margaritifera is nowadays residual, other human threats have aggravated the species conservation status, especially in Europe. This mussel presents a myriad of rare biological features, e.g. high longevity coupled with low senescence and Doubly Uniparental Inheritance of mitochondrial DNA, for which the underlying molecular mechanisms are poorly known. Here, the first draft genome assembly of M. margaritifera was produced using a combination of Illumina Paired-end and Mate-pair approaches. The genome assembly was 2.4 Gb long, possessing 105,185 scaffolds and a scaffold N50 length of 288,726 bp. The ab initio gene prediction allowed the identification of 35,119 protein-coding genes. This genome represents an essential resource for studying this species\u2019 unique biological and evolutionary features and ultimately will help to develop new tools to promote its conservation.<\/jats:p>","DOI":"10.1093\/dnares\/dsab002","type":"journal-article","created":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T16:14:31Z","timestamp":1616429671000},"source":"Crossref","is-referenced-by-count":25,"title":["The Crown Pearl: a draft genome assembly of the European freshwater pearl mussel\n                    <i>Margaritifera margaritifera<\/i>\n                    (Linnaeus, 1758)"],"prefix":"10.1093","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9973-4861","authenticated-orcid":false,"given":"Andr\u00e9","family":"Gomes-dos-Santos","sequence":"first","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos , S\/N, P 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre , 4169-007 Porto, Portugal"}]},{"given":"Manuel","family":"Lopes-Lima","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos , S\/N, P 4450-208 Matosinhos, Portugal"},{"name":"CIBIO\/InBIO\u2014Research Center in Biodiversity and Genetic Resources, Universidade do Porto, Campus Agr\u00e1rio de Vair\u00e3o, Rua Padre Armando Quintas , 4485-661 Vair\u00e3o, Portugal"},{"name":"IUCN SSC Mollusc Specialist Group, c\/o IUCN , Cambridge, England"}]},{"given":"Andr\u00e9 M","family":"Machado","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos , S\/N, P 4450-208 Matosinhos, Portugal"}]},{"given":"Ant\u00f3nio","family":"Marcos Ramos","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia Agr\u00edcola e Agro-alimentar do Alentejo (CEBAL), Instituto Polit\u00e9cnico de Beja (IPBeja) , 7801-908 Beja, Portugal"},{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development, CEBAL\u2014Centro de Biotecnologia Agr\u00edcola e Agro-Alimentar do Alentejo , 7801-908 Beja, Portugal"}]},{"given":"Ana","family":"Usi\u00e9","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia Agr\u00edcola e Agro-alimentar do Alentejo (CEBAL), Instituto Polit\u00e9cnico de Beja (IPBeja) , 7801-908 Beja, Portugal"},{"name":"MED\u2014Mediterranean Institute for Agriculture, Environment and Development, CEBAL\u2014Centro de Biotecnologia Agr\u00edcola e Agro-Alimentar do Alentejo , 7801-908 Beja, Portugal"}]},{"given":"Ivan N","family":"Bolotov","sequence":"additional","affiliation":[{"name":"Federal Center for Integrated Arctic Research, Russian Academy of Sciences , Arkhangelsk 163000, Russia"}]},{"given":"Ilya V","family":"Vikhrev","sequence":"additional","affiliation":[{"name":"Federal Center for Integrated Arctic Research, Russian Academy of Sciences , Arkhangelsk 163000, Russia"}]},{"given":"Sophie","family":"Breton","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Montreal , Montreal, Canada"}]},{"given":"L Filipe C","family":"Castro","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos , S\/N, P 4450-208 Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre , 4169-007 Porto, Portugal"}]},{"given":"Rute R","family":"da Fonseca","sequence":"additional","affiliation":[{"name":"Center for Macroecology, Evolution and Climate, GLOBE Institute, University of Copenhagen , 2100 Copenhagen, Denmark"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7698-3443","authenticated-orcid":false,"given":"Juergen","family":"Geist","sequence":"additional","affiliation":[{"name":"Aquatic Systems Biology Unit, Technical University of Munich, TUM School of Life Sciences , D-85354 Freising, Germany"}]},{"given":"Martin E","family":"\u00d6sterling","sequence":"additional","affiliation":[{"name":"Department of Environmental and Life Sciences\u2014Biology, Karlstad University , 651 88 Karlstad, Sweden"}]},{"given":"Vincent","family":"Pri\u00e9","sequence":"additional","affiliation":[{"name":"Research Associate, Institute of Systematics, Evolution, Biodiversity (ISYEB), National Museum of Natural History (MNHN), CNRS, SU, EPHE , 75005 Paris, France"}]},{"given":"Am\u00edlcar","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o de Montanha (CIMO), Instituto Polit\u00e9cnico de Bragan\u00e7a , Bragan\u00e7a, Portugal"}]},{"given":"Han Ming","family":"Gan","sequence":"additional","affiliation":[{"name":"GeneSEQ Sdn Bhd, Bandar Bukit Beruntung , Rawang 48300, Selangor, Malaysia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3585-4511","authenticated-orcid":false,"given":"Oleg","family":"Simakov","sequence":"additional","affiliation":[{"name":"Department of Neurosciences and Developmental Biology, University of Vienna , 1010 Vienna, Austria"}]},{"given":"Elsa","family":"Froufe","sequence":"additional","affiliation":[{"name":"CIIMAR\/CIMAR\u2014Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos , S\/N, P 4450-208 Matosinhos, Portugal"}]}],"member":"286","published-online":{"date-parts":[[2021,3,23]]},"reference":[{"key":"2022061815422435000_dsab002-B1","doi-asserted-by":"crossref","DOI":"10.5962\/bhl.title.47047","volume-title":"Die Perlnmuscheln und thre Perlen (Naturwissen-schaftlich und geschichtlich mit, Beruecksichtigung der Perlgewaesser Baerns)","author":"von Hessling","year":"1859"},{"key":"2022061815422435000_dsab002-B2","doi-asserted-by":"crossref","first-page":"580","DOI":"10.15506\/JoG.2015.34.7.580","article-title":"European freshwater pearls: part 1-Russia","volume":"34","author":"Strack","year":"2015","journal-title":"J. Gemmol"},{"key":"2022061815422435000_dsab002-B3","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1134\/S2079970512020025","article-title":"Historical geography of pearl fishing in rivers of the Southern White Sea Region (Arkhangelsk Oblast)","volume":"2","author":"Bespalaya","year":"2012","journal-title":"Reg. Res. 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