{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T22:59:40Z","timestamp":1770418780918,"version":"3.49.0"},"reference-count":111,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T00:00:00Z","timestamp":1655337600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The discovery of new bioactive compounds is an invaluable aid to the development of new drugs. Strategies for finding novel molecules can focus on the exploitation of less studied organisms and ecosystems such as planctomycetes and brackish habitats. The unique cell biology of the underexplored Planctomycetota mean it is of particular interest. In this study, we aimed to isolate planctomycetes from the estuary of the Tejo river (Portugal). To reach this goal, macroalgae, water and sediments were sampled and diverse media and isolation techniques applied. Sixty-nine planctomycetal strains were brought into pure culture. An analysis of the 16S rRNA genes found that the majority of the isolates were affiliated to the genus Rhodopirellula. Putative novel taxa belonging to genera Stieleria and Rhodopirellula were also isolated and characterized morphologically. Enterobacterial repetitive intergenic consensus fingerprinting analyses showed higher diversity and different genotypes within close strains. Relevant biosynthetic gene clusters were found in most isolates and acetone extracts from representative strains exhibited mild antimicrobial activities against Escherichia coli and Staphylococcus aureus. Our work has not only enlarged the number and diversity of cultured planctomycetes but has also shown the potential for the discovery of bioactive compounds from the novel taxa.<\/jats:p>","DOI":"10.1093\/femsec\/fiac066","type":"journal-article","created":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T19:52:45Z","timestamp":1655409165000},"source":"Crossref","is-referenced-by-count":14,"title":["Isolation, diversity and antimicrobial activity of planctomycetes from the Tejo river estuary (Portugal)"],"prefix":"10.1093","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5316-4493","authenticated-orcid":false,"given":"In\u00eas Rosado","family":"Vitorino","sequence":"first","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto , Rua do Campo Alegre s\/n\u00b0, 4169-007 Porto, Portugal"},{"name":"CIIMAR\/CIMAR, Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental, Universidade do Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos , S\/N, 4450-208 Matosinhos, Portugal"}]},{"given":"Alexandre","family":"Lobo-da-Cunha","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Celular, Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar, ICBAS, Universidade do Porto , Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}]},{"given":"V\u00edtor","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto , Rua do Campo Alegre s\/n\u00b0, 4169-007 Porto, Portugal"},{"name":"CIIMAR\/CIMAR, Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental, Universidade do Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos , S\/N, 4450-208 Matosinhos, Portugal"}]},{"given":"Francisca","family":"Vicente","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n MEDINA, Centro de Excelencia en Investigaci\u00f3n de Medicamentos Innovadores en Andaluc\u00eda , Avenida del Conocimiento 34, Parque Tecnol\u00f3gico de Ciencias de la Salud, 18016 Granada, Spain"}]},{"given":"Olga Maria","family":"Lage","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Faculdade de Ci\u00eancias, Universidade do Porto , Rua do Campo Alegre s\/n\u00b0, 4169-007 Porto, Portugal"},{"name":"CIIMAR\/CIMAR, Centro Interdisciplinar de Investiga\u00e7\u00e3o Marinha e Ambiental, Universidade do Porto, Terminal de Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos , S\/N, 4450-208 Matosinhos, Portugal"}]}],"member":"286","published-online":{"date-parts":[[2022,6,16]]},"reference":[{"key":"2022070119362792100_bib1","first-page":"277","article-title":"A bacterial tubulovesicular network","volume":"127","author":"Acehan","year":"2014","journal-title":"J Cell 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