{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T06:11:28Z","timestamp":1777183888721,"version":"3.51.4"},"reference-count":127,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T00:00:00Z","timestamp":1742256000000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Luxembourg National Research Fund"},{"DOI":"10.13039\/100012125","name":"ARF","doi-asserted-by":"publisher","award":["14583934"],"award-info":[{"award-number":["14583934"]}],"id":[{"id":"10.13039\/100012125","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Studying bacteria in anaerobic digestion (AD) is crucial for optimizing microbial processes. While abundant taxa are often studied, less abundant groups may harbour novel metabolic potential. This study fills the gap by focusing on the Planctomycetota phylum, known to encode diverse carbohydrate-active enzymes (CAZymes). Despite their common presence in diverse aerobic and anaerobic environments, their role in AD is relatively unexplored. We utilized both culture-dependent and culture-independent techniques to investigate the phylogenetic and metabolic diversity of Planctomycetota within AD reactors. Our findings revealed that among the diverse planctomycetotal operational taxonomic units present, only a few are prevalent and abundant community members. Planctomycetota share functional traits with e.g. Verrucomicrobiota exhibiting distinct CAZyme gene repertoires that indicates specialization in degrading algal polysaccharides and glycoproteins. To explore the planctomycetotal metabolic capabilities, we monitored their presence in algal-fed digesters. Additionally, we isolated a strain from mucin-based medium, revealing its genetic potential for a mixotrophic lifestyle. Based on the genomic analysis, we propose to introduce the Candidatus Luxemburgiella decessa gen. nov. sp. nov., belonging to the Thermoguttaceae family within the Pirellulales order of the Planctomycetia class. This study enhances our understanding of Planctomycetota in AD by highlighting their phylogenetic diversity and metabolic capabilities.<\/jats:p>","DOI":"10.1093\/femsec\/fiaf025","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T02:43:37Z","timestamp":1742265817000},"source":"Crossref","is-referenced-by-count":3,"title":["Insights into the phylogenetic and metabolic diversity of <i>Planctomycetota<\/i> in anaerobic digesters and the isolation of novel <i>Thermoguttaceae<\/i> species"],"prefix":"10.1093","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6713-8333","authenticated-orcid":false,"given":"Dominika","family":"Klimek","sequence":"first","affiliation":[{"name":"Environmental and Industrial Biotechnology, Luxembourg Institute of Science and Technology (LIST) , L-4970 Hautcharage ,","place":["Luxembourg"]},{"name":"Faculty of Science, Technology and Medicine (FSTM), University of Luxembourg , L-4364 Esch-sur-Alzette ,","place":["Luxembourg"]}]},{"given":"Malte","family":"Herold","sequence":"additional","affiliation":[{"name":"Environmental and Industrial Biotechnology, Luxembourg Institute of Science and Technology (LIST) , L-4970 Hautcharage ,","place":["Luxembourg"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5316-4493","authenticated-orcid":false,"given":"In\u00eas Rosado","family":"Vitorino","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Sciences, University of Porto , 4169-007 Porto ,","place":["Portugal"]}]},{"given":"Zuzana","family":"Dedova","sequence":"additional","affiliation":[{"name":"Environmental and Industrial Biotechnology, Luxembourg Institute of Science and Technology (LIST) , L-4970 Hautcharage ,","place":["Luxembourg"]}]},{"given":"Sebastien","family":"Lemaigre","sequence":"additional","affiliation":[{"name":"Environmental and Industrial Biotechnology, Luxembourg Institute of Science and Technology (LIST) , L-4970 Hautcharage ,","place":["Luxembourg"]}]},{"given":"Jimmy","family":"Roussel","sequence":"additional","affiliation":[{"name":"Environmental and Industrial Biotechnology, Luxembourg Institute of Science and Technology (LIST) , L-4970 Hautcharage ,","place":["Luxembourg"]}]},{"given":"Xavier","family":"Goux","sequence":"additional","affiliation":[{"name":"Environmental and Industrial Biotechnology, Luxembourg Institute of Science and Technology (LIST) , L-4970 Hautcharage ,","place":["Luxembourg"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3950-3970","authenticated-orcid":false,"given":"Olga Maria","family":"Lage","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Sciences, University of Porto , 4169-007 Porto ,","place":["Portugal"]},{"name":"Interdisciplinary Centre of Marine and Environmental Research (CIIMAR\/CIMAR) , 4450-208 Matosinhos ,","place":["Portugal"]}]},{"given":"Magdalena","family":"Calusinska","sequence":"additional","affiliation":[{"name":"Environmental and Industrial Biotechnology, Luxembourg Institute of Science and Technology (LIST) , L-4970 Hautcharage ,","place":["Luxembourg"]}]}],"member":"286","published-online":{"date-parts":[[2025,3,17]]},"reference":[{"key":"2025032219043372700_bib1","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1186\/1471-2148-9-118","article-title":"Insights into the evolution of sialic acid catabolism among bacteria","volume":"9","author":"Almagro-Moreno","year":"2009","journal-title":"BMC Evol Biol"},{"key":"2025032219043372700_bib2","doi-asserted-by":"publisher","first-page":"13219","DOI":"10.1038\/ncomms13219","article-title":"Thousands of microbial genomes shed light on interconnected biogeochemical processes in an aquifer system","volume":"7","author":"Anantharaman","year":"2016","journal-title":"Nat Commun"},{"key":"2025032219043372700_bib3","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1016\/j.biotechadv.2018.01.011","article-title":"Biogas upgrading and utilization: current status and perspectives","volume":"36","author":"Angelidaki","year":"2018","journal-title":"Biotechnol Adv"},{"key":"2025032219043372700_bib4","doi-asserted-by":"publisher","first-page":"2500","DOI":"10.1038\/s41467-020-16366-7","article-title":"Precise phylogenetic analysis of microbial isolates and genomes from metagenomes using PhyloPhlAn 3.0","volume":"11","author":"Asnicar","year":"2020","journal-title":"Nat Commun"},{"key":"2025032219043372700_bib5","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1186\/1471-2164-9-75","article-title":"The RAST server: rapid annotations using subsystems technology","volume":"9","author":"Aziz","year":"2008","journal-title":"BMC Genomics"},{"key":"2025032219043372700_bib6","doi-asserted-by":"publisher","first-page":"1525","DOI":"10.1093\/jxb\/erm297","article-title":"Multiple Rubisco forms in proteobacteria: their functional significance in relation to CO2 acquisition by the CBB cycle","volume":"59","author":"Badger","year":"2008","journal-title":"J Exp Bot"},{"key":"2025032219043372700_bib7","doi-asserted-by":"publisher","first-page":"133776","DOI":"10.1016\/j.chemosphere.2022.133776","article-title":"The effect of substituting energy crop with agricultural waste on the dynamics of bacterial communities in a two-stage anaerobic digester","volume":"294","author":"Bellucci","year":"2022","journal-title":"Chemosphere"},{"key":"2025032219043372700_bib8","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1016\/j.mib.2021.09.008","article-title":"Microbial communities and their enzymes facilitate degradation of recalcitrant polymers in anaerobic digestion","volume":"64","author":"Blair","year":"2021","journal-title":"Curr Opin Microbiol"},{"key":"2025032219043372700_bib9","doi-asserted-by":"publisher","first-page":"14853","DOI":"10.1038\/ncomms14853","article-title":"Determining the bacterial cell biology of planctomycetes","volume":"8","author":"Boedeker","year":"2017","journal-title":"Nat Commun"},{"key":"2025032219043372700_bib10","doi-asserted-by":"publisher","first-page":"3221","DOI":"10.1128\/AEM.07307-11","article-title":"Microbial scout hypothesis, stochastic exit from dormancy, and the nature of slow growers","volume":"78","author":"Buerger","year":"2012","journal-title":"Appl Environ Microbiol"},{"key":"2025032219043372700_bib11","doi-asserted-by":"publisher","first-page":"1000","DOI":"10.1016\/j.renene.2021.09.100","article-title":"Biogas composition from agricultural sources and organic fraction of municipal solid waste","volume":"181","author":"Calbry-Muzyka","year":"2022","journal-title":"Renew Energy"},{"key":"2025032219043372700_bib12","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1186\/s13068-018-1195-8","article-title":"A year of monitoring 20 mesophilic full-scale bioreactors reveals the existence of stable but different core microbiomes in bio-waste and wastewater anaerobic digestion systems","volume":"11","author":"Calusinska","year":"2018","journal-title":"Biotechnol Biofuels"},{"key":"2025032219043372700_bib13","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1186\/s13068-020-01679-y","article-title":"New insights from the biogas microbiome by comprehensive genome-resolved metagenomics of nearly 1600 species originating from multiple anaerobic digesters","volume":"13","author":"Campanaro","year":"2020","journal-title":"Biotechnol Biofuels"},{"key":"2025032219043372700_bib14","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1016\/j.copbio.2015.01.008","article-title":"Microbial management of anaerobic digestion: exploiting the microbiome-functionality nexus","volume":"33","author":"Carballa","year":"2015","journal-title":"Curr Opin Biotechnol"},{"key":"2025032219043372700_bib15","doi-asserted-by":"publisher","first-page":"1082","DOI":"10.1111\/apm.12087","article-title":"Molecular diversity of the planctomycetes in the human gut microbiota in France and Senegal","volume":"121","author":"Cayrou","year":"2013","journal-title":"APMIS"},{"key":"2025032219043372700_bib16","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s40793-023-00545-2","article-title":"A unified compendium of prokaryotic and viral genomes from over 300 anaerobic digestion microbiomes","volume":"19","author":"Centurion","year":"2024","journal-title":"Environ Microbiome"},{"key":"2025032219043372700_bib17","doi-asserted-by":"publisher","first-page":"1925","DOI":"10.1093\/bioinformatics\/btz848","article-title":"GTDB-Tk: a toolkit to classify genomes with the Genome Taxonomy Database","volume":"36","author":"Chaumeil","year":"2020","journal-title":"Bioinformatics"},{"key":"2025032219043372700_bib18","doi-asserted-by":"publisher","first-page":"106002","DOI":"10.1016\/j.marenvres.2023.106002","article-title":"Insights into autotrophic carbon fixation strategies through metagonomics in the sediments of seagrass beds","volume":"188","author":"Chi","year":"2023","journal-title":"Mar Environ Res"},{"key":"2025032219043372700_bib19","doi-asserted-by":"publisher","first-page":"1203","DOI":"10.1038\/s41592-023-01940-w","article-title":"CheckM2: a rapid, scalable and accurate tool for assessing microbial genome quality using machine learning","volume":"20","author":"Chklovski","year":"2023","journal-title":"Nat Methods"},{"key":"2025032219043372700_bib20","doi-asserted-by":"publisher","first-page":"7354","DOI":"10.1128\/AEM.69.12.7354-7363.2003","article-title":"Molecular evidence for novel planctomycete diversity in a municipal wastewater treatment plant","volume":"69","author":"Chouari","year":"2003","journal-title":"Appl Environ Microbiol"},{"key":"2025032219043372700_bib21","doi-asserted-by":"publisher","first-page":"1314","DOI":"10.1038\/s41587-019-0260-6","article-title":"Targeted isolation and cultivation of uncultivated bacteria by reverse genomics","volume":"37","author":"Cross","year":"2019","journal-title":"Nat Biotechnol"},{"key":"2025032219043372700_bib22","doi-asserted-by":"publisher","first-page":"1450","DOI":"10.1038\/s41564-023-01407-w","article-title":"A genetic system for Akkermansia muciniphila reveals a role for mucin foraging in gut colonization and host sterol biosynthesis gene expression","volume":"8","author":"Davey","year":"2023","journal-title":"Nat Microbiol"},{"key":"2025032219043372700_bib23","doi-asserted-by":"publisher","first-page":"126050","DOI":"10.1016\/j.syapm.2019.126050","article-title":"Lacipirellula parvula gen. nov., sp. nov., representing a lineage of planctomycetes widespread in low-oxygen habitats, description of the family Lacipirellulaceae fam. nov. and proposal of the orders Pirellulales ord. nov., Gemmatales ord. nov. and Isosphaerales ord. nov","volume":"43","author":"Dedysh","year":"2020","journal-title":"Syst Appl Microbiol"},{"key":"2025032219043372700_bib24","doi-asserted-by":"publisher","first-page":"804","DOI":"10.1038\/s41564-018-0176-9","article-title":"Nitrogen-fixing populations of planctomycetes and proteobacteria are abundant in surface ocean metagenomes","volume":"3","author":"Delmont","year":"2018","journal-title":"Nat Microbiol"},{"key":"2025032219043372700_bib25","doi-asserted-by":"publisher","first-page":"D571","DOI":"10.1093\/nar\/gkab1045","article-title":"The carbohydrate-active enzyme database: functions and literature","volume":"50","author":"Drula","year":"2022","journal-title":"Nucleic Acids Res"},{"key":"2025032219043372700_bib26","doi-asserted-by":"publisher","first-page":"5361","DOI":"10.1101\/2023.08.24.554448","article-title":"MiDAS 5: global diversity of bacteria and archaea in anaerobic digesters","volume":"15","author":"Dueholm","year":"2023","journal-title":"Microbiology"},{"key":"2025032219043372700_bib27","doi-asserted-by":"publisher","first-page":"e1002195","DOI":"10.1371\/journal.pcbi.1002195","article-title":"Accelerated profile HMM searches","volume":"7","author":"Eddy","year":"2011","journal-title":"PLoS Comput Biol"},{"key":"2025032219043372700_bib28","doi-asserted-by":"publisher","first-page":"4707","DOI":"10.1128\/AEM.00591-07","article-title":"Phylogenetic and metabolic diversity of planctomycetes from anaerobic, sulfide- and sulfur-rich Zodletone Spring, Oklahoma","volume":"73","author":"Elshahed","year":"2007","journal-title":"Appl Environ Microbiol"},{"key":"2025032219043372700_bib29","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1038\/s41587-020-0439-x","article-title":"The nf-core framework for community-curated bioinformatics pipelines","volume":"38","author":"Ewels","year":"2020","journal-title":"Nat Biotechnol"},{"key":"2025032219043372700_bib30","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/j.ygeno.2017.10.007","article-title":"Planctomycetes attached to algal surfaces: insight into their genomes","volume":"110","author":"Faria","year":"2018","journal-title":"Genomics"},{"key":"2025032219043372700_bib31","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.963456","article-title":"Fish gut-associated bacterial communities in a tropical lagoon (Aghien lagoon, Ivory Coast)","volume":"13","author":"Gallet","year":"2022","journal-title":"Front Microbiol"},{"key":"2025032219043372700_bib32","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00037","article-title":"FeGenie: a comprehensive tool for the identification of iron genes and iron gene neighborhoods in genome and metagenome assemblies","volume":"11","author":"Garber","year":"2020","journal-title":"Front Microbiol"},{"key":"2025032219043372700_bib33","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41522-022-00309-9","article-title":"Functional and phylogenetic analyses of camel rumen microbiota associated with different lignocellulosic substrates","volume":"8","author":"Gharechahi","year":"2022","journal-title":"npj Biofilms Microbiomes"},{"key":"2025032219043372700_bib34","doi-asserted-by":"publisher","first-page":"181","DOI":"10.3390\/jmse12010181","article-title":"Fucanases related to the GH107 family from members of the PVC superphylum","volume":"12","author":"Gonzalez","year":"2024","journal-title":"JMSE"},{"key":"2025032219043372700_bib35","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1186\/s13068-015-0309-9","article-title":"Microbial community dynamics in replicate anaerobic digesters exposed sequentially to increasing organic loading rate, acidosis, and process recovery","volume":"8","author":"Goux","year":"2015","journal-title":"Biotechnol Biofuels"},{"key":"2025032219043372700_bib36","doi-asserted-by":"publisher","first-page":"100764","DOI":"10.1016\/j.biteb.2021.100764","article-title":"Insight into microbial community diversity and composition of two-stage anaerobic digestion: focusing methanogenic stage","volume":"15","author":"Hailu","year":"2021","journal-title":"Bioresour Technol Rep"},{"key":"2025032219043372700_bib37","doi-asserted-by":"publisher","first-page":"9057","DOI":"10.1038\/s41598-018-27350-z","article-title":"The impact of the Deepwater Horizon blowout on historic shipwreck-associated sediment microbiomes in the northern Gulf of Mexico","volume":"8","author":"Hamdan","year":"2018","journal-title":"Sci Rep"},{"key":"2025032219043372700_bib38","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2024.1341359","article-title":"Host species of freshwater snails within the same freshwater ecosystem shapes the intestinal microbiome","volume":"12","author":"Hu","year":"2024","journal-title":"Front Ecol Evol"},{"key":"2025032219043372700_bib39","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1186\/1754-6834-5-86","article-title":"Biogas from macroalgae: is it time to revisit the idea?","volume":"5","author":"Hughes","year":"2012","journal-title":"Biotechnol Biofuels"},{"key":"2025032219043372700_bib40","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1186\/1471-2105-11-119","article-title":"Prodigal: prokaryotic gene recognition and translation initiation site identification","volume":"11","author":"Hyatt","year":"2010","journal-title":"BMC Bioinf"},{"key":"2025032219043372700_bib41","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1007\/s10482-017-0973-9","article-title":"Metatranscriptomics reveals the hydrolytic potential of peat-inhabiting Planctomycetes","volume":"111","author":"Ivanova","year":"2018","journal-title":"Antonie Van Leeuwenhoek"},{"key":"2025032219043372700_bib42","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1007\/s10482-013-0007-1","article-title":"From genome mining to phenotypic microarrays: planctomycetes as source for novel bioactive molecules","volume":"104","author":"Jeske","year":"2013","journal-title":"Antonie Van Leeuwenhoek"},{"key":"2025032219043372700_bib43","doi-asserted-by":"publisher","first-page":"113458","DOI":"10.1016\/j.marpolbul.2022.113458","article-title":"Insights into carbon-fixation pathways through metagonomics in the sediments of deep-sea cold seeps","volume":"176","author":"Jiang","year":"2022","journal-title":"Mar Pollut Bull"},{"key":"2025032219043372700_bib44","doi-asserted-by":"publisher","first-page":"853","DOI":"10.1038\/ismej.2016.174","article-title":"Where less may be more: how the rare biosphere pulls ecosystems strings","volume":"11","author":"Jousset","year":"2017","journal-title":"ISME J"},{"key":"2025032219043372700_bib45","doi-asserted-by":"publisher","first-page":"519301","DOI":"10.3389\/fcimb.2020.519301","article-title":"Planctomycetes as host-associated bacteria: a perspective that holds promise for their future isolations, by mimicking their native environmental niches in clinical microbiology laboratories","volume":"10","author":"Kabor\u00e9","year":"2020","journal-title":"Front Cell Infect Microbiol"},{"key":"2025032219043372700_bib46","doi-asserted-by":"publisher","first-page":"D587","DOI":"10.1093\/nar\/gkac963","article-title":"KEGG for taxonomy-based analysis of pathways and genomes","volume":"51","author":"Kanehisa","year":"2023","journal-title":"Nucleic Acids Res"},{"key":"2025032219043372700_bib47","doi-asserted-by":"publisher","first-page":"726","DOI":"10.1016\/j.jmb.2015.11.006","article-title":"BlastKOALA and GhostKOALA: KEGG tools for functional characterization of genome and metagenome sequences","volume":"428","author":"Kanehisa","year":"2016","journal-title":"J Mol Biol"},{"key":"2025032219043372700_bib48","doi-asserted-by":"publisher","first-page":"e7359","DOI":"10.7717\/peerj.7359","article-title":"MetaBAT 2: an adaptive binning algorithm for robust and efficient genome reconstruction from metagenome assemblies","volume":"7","author":"Kang","year":"2019","journal-title":"PeerJ"},{"key":"2025032219043372700_bib49","doi-asserted-by":"publisher","DOI":"10.3389\/fbinf.2022.918853","article-title":"microTrait: a toolset for a trait-based representation of microbial genomes","volume":"2","author":"Karaoz","year":"2022","journal-title":"Front Bioinform"},{"key":"2025032219043372700_bib50","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1016\/B978-0-12-398264-3.00003-6","article-title":"Anammox\u2014growth physiology, cell biology, and metabolism","volume-title":"Advances in Microbial Physiology","author":"Kartal","year":"2012"},{"key":"2025032219043372700_bib51","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1186\/s13068-022-02207-w","article-title":"Adaptation of a microbial community to demand-oriented biological methanation","volume":"15","author":"Khesali\u00a0Aghtaei","year":"2022","journal-title":"Biotechnol Biofuels"},{"key":"2025032219043372700_bib52","doi-asserted-by":"publisher","first-page":"126522","DOI":"10.1016\/j.syapm.2024.126522","article-title":"Anaerobaca lacustris gen. nov., sp. nov., an obligately anaerobic planctomycete of the widespread SG8-4 group, isolated from a coastal lake, and proposal of Anaerobacaceae fam. nov","volume":"47","author":"Khomyakova","year":"2024","journal-title":"Syst Appl Microbiol"},{"key":"2025032219043372700_bib53","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1186\/s12864-024-10413-z","article-title":"Comparative genomic analysis of planctomycetota potential for polysaccharide degradation identifies biotechnologically relevant microbes","volume":"25","author":"Klimek","year":"2024","journal-title":"BMC Genomics"},{"key":"2025032219043372700_bib54","doi-asserted-by":"publisher","first-page":"1260","DOI":"10.1111\/j.1462-2920.2007.01540.x","article-title":"Novel lineages of Planctomycetes densely colonize the alkaline gut of soil-feeding termites (Cubitermes spp.)","volume":"10","author":"K\u00f6hler","year":"2008","journal-title":"Environ Microbiol"},{"key":"2025032219043372700_bib55","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1038\/s41587-019-0072-8","article-title":"Assembly of long, error-prone reads using repeat graphs","volume":"37","author":"Kolmogorov","year":"2019","journal-title":"Nat Biotechnol"},{"key":"2025032219043372700_bib56","doi-asserted-by":"publisher","first-page":"2399","DOI":"10.1038\/ismej.2017.113","article-title":"Uncultivated microbes in need of their own taxonomy","volume":"11","author":"Konstantinidis","year":"2017","journal-title":"ISME J"},{"key":"2025032219043372700_bib57","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1007\/s11783-018-1037-8","article-title":"Biogas and its opportunities\u2014a review","volume":"12","author":"Kougias","year":"2018","journal-title":"Front Environ Sci Eng"},{"key":"2025032219043372700_bib58","doi-asserted-by":"publisher","first-page":"5692","DOI":"10.1016\/j.biortech.2011.02.108","article-title":"Potential impact of process parameters upon the bacterial diversity in the mesophilic anaerobic digestion of beet silage","volume":"102","author":"Krakat","year":"2011","journal-title":"Bioresour Technol"},{"key":"2025032219043372700_bib59","doi-asserted-by":"crossref","first-page":"405","DOI":"10.3389\/fmicb.2012.00405","article-title":"Bringing planctomycetes into pure culture","volume":"3","author":"Lage","year":"2012","journal-title":"Front Microbiol"},{"key":"2025032219043372700_bib60","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1007\/s10482-013-9969-2","article-title":"Insights into the ultrastructural morphology of novel planctomycetes","volume":"104","author":"Lage","year":"2013","journal-title":"Antonie Van Leeuwenhoek"},{"key":"2025032219043372700_bib61","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1111\/j.1574-6941.2011.01168.x","article-title":"Planctomycetes diversity associated with macroalgae","volume":"78","author":"Lage","year":"2011","journal-title":"FEMS Microbiol Ecol"},{"key":"2025032219043372700_bib62","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00267","article-title":"Planctomycetes and macroalgae, a striking association","volume":"5","author":"Lage","year":"2014","journal-title":"Front Microbiol"},{"key":"2025032219043372700_bib63","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat Methods"},{"key":"2025032219043372700_bib64","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1186\/s13068-023-02438-5","article-title":"Potential of acetic acid to restore methane production in anaerobic reactors critically intoxicated by ammonia as evidenced by metabolic and microbial monitoring","volume":"16","author":"Lemaigre","year":"2023","journal-title":"Biotechnol Biofuels"},{"key":"2025032219043372700_bib65","doi-asserted-by":"publisher","first-page":"W293","DOI":"10.1093\/nar\/gkab301","article-title":"Interactive Tree of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation","volume":"49","author":"Letunic","year":"2021","journal-title":"Nucleic Acids Res"},{"key":"2025032219043372700_bib66","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.ymeth.2016.02.020","article-title":"MEGAHIT v1.0: a fast and scalable metagenome assembler driven by advanced methodologies and community practices","volume":"102","author":"Li","year":"2016","journal-title":"Methods"},{"key":"2025032219043372700_bib67","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01337","article-title":"Substrate type and free ammonia determine bacterial community structure in full-scale mesophilic anaerobic digesters treating cattle or swine manure","volume":"6","author":"Li","year":"2015","journal-title":"Front Microbiol"},{"key":"2025032219043372700_bib68","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1016\/j.biortech.2015.04.029","article-title":"Effect of microalgae supplementation on the silage quality and anaerobic digestion performance of Manyflower silvergrass","volume":"189","author":"Li","year":"2015","journal-title":"Bioresour Technol"},{"key":"2025032219043372700_bib69","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1267152","article-title":"Metagenomic survey reveals global distribution and evolution of microbial sialic acid catabolism","volume":"14","author":"Li","year":"2023","journal-title":"Front Microbiol"},{"key":"2025032219043372700_bib70","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/bs.aibe.2020.04.001","article-title":"Chapter one\u2014the microbiome driving anaerobic digestion and microbial analysis","volume-title":"Advances in Bioenergy","author":"Lim","year":"2020"},{"key":"2025032219043372700_bib71","first-page":"994","article-title":"Isolation and characterization of Thermopirellula anaerolimosa gen. nov., sp. nov., an obligate anaerobic hydrogen-producing bacterium of the phylum Planctomycetes","volume":"52","author":"Liu","year":"2012","journal-title":"Wei Sheng Wu Xue Bao"},{"key":"2025032219043372700_bib72","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1038\/nrmicro3400","article-title":"Ecology and exploration of the rare biosphere","volume":"13","author":"Lynch","year":"2015","journal-title":"Nat Rev Microbiol"},{"key":"2025032219043372700_bib73","doi-asserted-by":"publisher","first-page":"118565","DOI":"10.1016\/j.watres.2022.118565","article-title":"The in-situ release of algal bloom populations and the role of prokaryotic communities in their establishment and growth","volume":"219","author":"Ma","year":"2022","journal-title":"Water Res"},{"key":"2025032219043372700_bib74","doi-asserted-by":"publisher","first-page":"e01755","DOI":"10.1128\/aem.01755-22","article-title":"Human gut metagenomes encode diverse GH156 sialidases","volume":"88","author":"Mann","year":"2022","journal-title":"Appl Environ Microbiol"},{"key":"2025032219043372700_bib75","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1016\/B978-0-12-415847-4.00014-8","article-title":"Mucins and mucus","volume-title":"Mucosal Immunology","author":"McGuckin","year":"2015"},{"key":"2025032219043372700_bib76","doi-asserted-by":"publisher","first-page":"12265","DOI":"10.1021\/acs.est.8b02822","article-title":"Distribution of extracellular flavins in a coastal marine basin and their relationship to redox gradients and microbial community members","volume":"52","author":"Monteverde","year":"2018","journal-title":"Environ Sci Technol"},{"key":"2025032219043372700_bib77","doi-asserted-by":"publisher","first-page":"5323","DOI":"10.1007\/s00253-018-9003-8","article-title":"Seeking key microorganisms for enhancing methane production in anaerobic digestion of waste sewage sludge","volume":"102","author":"Mustapha","year":"2018","journal-title":"Appl Microbiol Biotechnol"},{"key":"2025032219043372700_bib78","doi-asserted-by":"publisher","first-page":"3730","DOI":"10.1016\/j.biortech.2010.11.119","article-title":"A meta-analysis of the microbial diversity observed in anaerobic digesters","volume":"102","author":"Nelson","year":"2011","journal-title":"Bioresour Technol"},{"key":"2025032219043372700_bib79","doi-asserted-by":"publisher","first-page":"824","DOI":"10.1101\/gr.213959.116","article-title":"metaSPAdes: a new versatile metagenomic assembler","volume":"27","author":"Nurk","year":"2017","journal-title":"Genome Res"},{"key":"2025032219043372700_bib80","doi-asserted-by":"publisher","first-page":"2864","DOI":"10.1038\/ismej.2017.126","article-title":"dRep: a tool for fast and accurate genomic comparisons that enables improved genome recovery from metagenomes through de-replication","volume":"11","author":"Olm","year":"2017","journal-title":"ISME J"},{"key":"2025032219043372700_bib81","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.005056","article-title":"Valid publication of the names of forty-two phyla of prokaryotes","volume":"71","author":"Oren","year":"2021","journal-title":"Int J Syst Evol Microbiol"},{"key":"2025032219043372700_bib82","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1016\/j.bpg.2017.10.001","article-title":"Action and function of Akkermansia muciniphila in microbiome ecology, health and disease","volume":"31","author":"Ottman","year":"2017","journal-title":"Best Pract Res Clin Gastroenterol"},{"key":"2025032219043372700_bib83","doi-asserted-by":"crossref","unstructured":"Pan S, Zhu C, Zhao X-M. \u00a0et al. \u00a0A deep siamese neural network improves metagenome-assembled genomes in microbiome datasets across different environments. Nat Commun. 2022;13:2326. 10.1038\/s41467-022-29843-y.","DOI":"10.1038\/s41467-022-29843-y"},{"key":"2025032219043372700_bib84","doi-asserted-by":"publisher","first-page":"996","DOI":"10.1038\/nbt.4229","article-title":"A standardized bacterial taxonomy based on genome phylogeny substantially revises the tree of life","volume":"36","author":"Parks","year":"2018","journal-title":"Nat Biotechnol"},{"key":"2025032219043372700_bib85","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.1101\/gr.186072.114","article-title":"CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes","volume":"25","author":"Parks","year":"2015","journal-title":"Genome Res"},{"key":"2025032219043372700_bib86","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00231","article-title":"The link between the ecology of the prokaryotic rare biosphere and its biotechnological potential","volume":"11","author":"Pascoal","year":"2020","journal-title":"Front Microbiol"},{"key":"2025032219043372700_bib87","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1146\/annurev-marine-120710-100948","article-title":"The rare bacterial biosphere","volume":"4","author":"Pedr\u00f3s-Ali\u00f3","year":"2012","journal-title":"Annu Rev Mar Sci"},{"key":"2025032219043372700_bib88","doi-asserted-by":"publisher","first-page":"137150","DOI":"10.1016\/j.chemosphere.2022.137150","article-title":"Evaluation of digestate-derived biochar to alleviate ammonia inhibition during long-term anaerobic digestion of food waste","volume":"311","author":"Peng","year":"2023","journal-title":"Chemosphere"},{"key":"2025032219043372700_bib89","doi-asserted-by":"publisher","first-page":"126523","DOI":"10.1016\/j.syapm.2024.126523","article-title":"Genomic and functional insights of a mucin foraging Rhodopirellula halodulae sp. nov","volume":"47","author":"Pk","year":"2024","journal-title":"Syst Appl Microbiol"},{"key":"2025032219043372700_bib90","doi-asserted-by":"publisher","first-page":"e102","DOI":"10.1002\/cpbi.102","article-title":"Using SPAdes De Novo Assembler","volume":"70","author":"Prjibelski","year":"2020","journal-title":"Curr Protoc Bioinformatics"},{"key":"2025032219043372700_bib91","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1007\/s00248-019-01403-w","article-title":"Dynamics of abundant and rare bacteria during degradation of lignocellulose from sugarcane biomass","volume":"79","author":"Puentes-T\u00e9llez","year":"2020","journal-title":"Microb Ecol"},{"key":"2025032219043372700_bib92","doi-asserted-by":"publisher","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: improved data processing and web-based tools","volume":"41","author":"Quast","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"2025032219043372700_bib93","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02645-17","article-title":"Genome analysis of fimbriiglobus ruber SP5T, a planctomycete with confirmed chitinolytic capability","volume":"84","author":"Ravin","year":"2018","journal-title":"Appl Environ Microbiol"},{"key":"2025032219043372700_bib94","doi-asserted-by":"publisher","first-page":"D503","DOI":"10.1093\/nar\/gkt953","article-title":"MEROPS: the database of proteolytic enzymes, their substrates and inhibitors","volume":"42","author":"Rawlings","year":"2014","journal-title":"Nucl Acids Res"},{"key":"2025032219043372700_bib95","doi-asserted-by":"publisher","first-page":"1640","DOI":"10.1038\/ismej.2017.26","article-title":"An alternative polysaccharide uptake mechanism of marine bacteria","volume":"11","author":"Reintjes","year":"2017","journal-title":"ISME J"},{"key":"2025032219043372700_bib96","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1038\/s41598-019-56978-8","article-title":"Non-essentiality of canonical cell division genes in the planctomycete Planctopirus limnophila","volume":"10","author":"Rivas-Marin","year":"2020","journal-title":"Sci Rep"},{"key":"2025032219043372700_bib97","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00731","article-title":"Genome-centric analysis of microbial populations enriched by hydraulic fracture fluid additives in a coal bed methane production well","volume":"7","author":"Robbins","year":"2016","journal-title":"Front Microbiol"},{"key":"2025032219043372700_bib98","doi-asserted-by":"publisher","first-page":"e13904","DOI":"10.1111\/1755-0998.13904","article-title":"MuDoGeR: multi-domain genome recovery from metagenomes made easy","volume":"24","author":"Rocha","year":"2024","journal-title":"Mol Ecol Resour"},{"key":"2025032219043372700_bib99","doi-asserted-by":"publisher","DOI":"10.7287\/peerj.preprints.1900v1","article-title":"The enveomics collection: a toolbox for specialized analyses of microbial genomes and metagenomes","author":"Rodriguez-R","year":"2016","journal-title":"PeerJ Inc"},{"key":"2025032219043372700_bib100","doi-asserted-by":"publisher","first-page":"ycad005","DOI":"10.1093\/ismeco\/ycad005","article-title":"Metagenome-assembled genomes of deep-sea sediments: changes in microbial functional potential lag behind redox transitions","volume":"4","author":"Schauberger","year":"2024","journal-title":"ISME Commun"},{"key":"2025032219043372700_bib101","doi-asserted-by":"publisher","first-page":"7537","DOI":"10.1128\/AEM.01541-09","article-title":"Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities","volume":"75","author":"Schloss","year":"2009","journal-title":"Appl Environ Microbiol"},{"key":"2025032219043372700_bib102","doi-asserted-by":"publisher","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: rapid prokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"2025032219043372700_bib103","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01371-14","article-title":"Conditionally rare taxa disproportionately contribute to temporal changes in microbial diversity","volume":"5","author":"Shade","year":"2014","journal-title":"mBio"},{"key":"2025032219043372700_bib104","doi-asserted-by":"publisher","first-page":"1833","DOI":"10.1038\/s41467-023-37533-6","article-title":"Sialidases and fucosidases of Akkermansia muciniphila are crucial for growth on mucin and nutrient sharing with mucus-associated gut bacteria","volume":"14","author":"Shuoker","year":"2023","journal-title":"Nat Commun"},{"key":"2025032219043372700_bib105","doi-asserted-by":"publisher","first-page":"1966","DOI":"10.1038\/s41396-023-01504-y","article-title":"Syntrophic entanglements for propionate and acetate oxidation under thermophilic and high-ammonia conditions","volume":"17","author":"Singh","year":"2023","journal-title":"ISME J"},{"key":"2025032219043372700_bib106","doi-asserted-by":"publisher","first-page":"760","DOI":"10.1099\/ijs.0.000009","article-title":"Thermogutta terrifontis gen. nov., sp. nov. and Thermogutta hypogea sp. nov., thermophilic anaerobic representatives of the phylum Planctomycetes","volume":"65","author":"Slobodkina","year":"2015","journal-title":"Int J Syst Evol Microbiol"},{"key":"2025032219043372700_bib107","doi-asserted-by":"publisher","first-page":"34212","DOI":"10.1038\/srep34212","article-title":"HydDB: a web tool for hydrogenase classification and analysis","volume":"6","author":"S\u00f8ndergaard","year":"2016","journal-title":"Sci Rep"},{"key":"2025032219043372700_bib108","doi-asserted-by":"publisher","first-page":"2438","DOI":"10.1111\/1462-2920.14253","article-title":"Genome biology of a novel lineage of planctomycetes widespread in anoxic aquatic environments","volume":"20","author":"Spring","year":"2018","journal-title":"Environ Microbiol"},{"key":"2025032219043372700_bib109","doi-asserted-by":"publisher","first-page":"D647","DOI":"10.1093\/nar\/gkac977","article-title":"SulfAtlas, the sulfatase database: state of the art and new developments","volume":"51","author":"Stam","year":"2023","journal-title":"Nucleic Acids Res"},{"key":"2025032219043372700_bib110","doi-asserted-by":"publisher","first-page":"953","DOI":"10.1038\/s41587-019-0202-3","article-title":"Compendium of 4941 rumen metagenome-assembled genomes for rumen microbiome biology and enzyme discovery","volume":"37","author":"Stewart","year":"2019","journal-title":"Nat Biotechnol"},{"key":"2025032219043372700_bib111","doi-asserted-by":"crossref","first-page":"628301","DOI":"10.3389\/fmicb.2021.628301","article-title":"Organic matter type defines the composition of active microbial communities originating from anoxic Baltic Sea sediments","volume":"12","author":"Suominen","year":"2021","journal-title":"Front Microbiol"},{"key":"2025032219043372700_bib112","doi-asserted-by":"publisher","first-page":"100120","DOI":"10.1016\/j.mtsust.2022.100120","article-title":"Efficient utilization and management of seaweed biomass for biogas production","volume":"18","author":"Thakur","year":"2022","journal-title":"Mater Tod Sustain"},{"key":"2025032219043372700_bib113","doi-asserted-by":"publisher","first-page":"3144","DOI":"10.1111\/1462-2920.13382","article-title":"Genome-centric resolution of microbial diversity, metabolism and interactions in anaerobic digestion","volume":"18","author":"Vanwonterghem","year":"2016","journal-title":"Environ Microbiol"},{"key":"2025032219043372700_bib114","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1186\/s13068-022-02125-x","article-title":"In silico evaluation of a targeted metaproteomics strategy for broad screening of cellulolytic enzyme capacities in anaerobic microbiome bioreactors","volume":"15","author":"Villalobos\u00a0Solis","year":"2022","journal-title":"Biotechnol Biofuels"},{"key":"2025032219043372700_bib115","doi-asserted-by":"publisher","first-page":"2078","DOI":"10.3390\/microorganisms9102078","article-title":"Novel and conventional isolation techniques to obtain planctomycetes from marine environments","volume":"9","author":"Vitorino","year":"2021","journal-title":"Microorganisms"},{"key":"2025032219043372700_bib116","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1007\/s10482-021-01699-0","article-title":"The planctomycetia: an overview of the currently largest class within the phylum planctomycetes","volume":"115","author":"Vitorino","year":"2022","journal-title":"Antonie Van Leeuwenhoek"},{"key":"2025032219043372700_bib117","doi-asserted-by":"publisher","first-page":"4607","DOI":"10.1128\/AEM.00055-15","article-title":"Stable-isotope probing identifies uncultured planctomycetes as primary degraders of a complex heteropolysaccharide in soil","volume":"81","author":"Wang","year":"2015","journal-title":"Appl Environ Microbiol"},{"key":"2025032219043372700_bib118","doi-asserted-by":"publisher","first-page":"e03321","DOI":"10.1128\/AEM.03321-16","article-title":"Quantifying the importance of the rare biosphere for microbial community response to organic pollutants in a freshwater ecosystem","volume":"83","author":"Wang","year":"2017","journal-title":"Appl Environ Microbiol"},{"key":"2025032219043372700_bib119","doi-asserted-by":"publisher","first-page":"fuab057","DOI":"10.1093\/femsre\/fuab057","article-title":"Syntrophic propionate-oxidizing bacteria in methanogenic systems","volume":"46","author":"Westerholm","year":"2022","journal-title":"FEMS Microbiol Rev"},{"key":"2025032219043372700_bib120","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01293","article-title":"Ecological functions of agricultural soil bacteria and microeukaryotes in chitin degradation: a case study","volume":"10","author":"Wieczorek","year":"2019","journal-title":"Front Microbiol"},{"key":"2025032219043372700_bib121","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1038\/s41564-019-0588-1","article-title":"Cultivation and functional characterization of 79 Planctomycetes uncovers their unique biology","volume":"5","author":"Wiegand","year":"2020","journal-title":"Nat Microbiol"},{"key":"2025032219043372700_bib122","doi-asserted-by":"publisher","first-page":"332","DOI":"10.1016\/j.biortech.2017.05.070","article-title":"Microbial monitoring of ammonia removal in a UASB reactor treating pre-digested chicken manure with anaerobic granular inoculum","volume":"241","author":"Yangin-Gomec","year":"2017","journal-title":"Bioresour Technol"},{"key":"2025032219043372700_bib123","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1038\/nrmicro3330","article-title":"Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences","volume":"12","author":"Yarza","year":"2014","journal-title":"Nat Rev Microbiol"},{"key":"2025032219043372700_bib124","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1007\/s10482-017-0844-4","article-title":"A large-scale evaluation of algorithms to calculate average nucleotide identity","volume":"110","author":"Yoon","year":"2017","journal-title":"Antonie Van Leeuwenhoek"},{"key":"2025032219043372700_bib125","doi-asserted-by":"publisher","first-page":"101533","DOI":"10.1016\/j.algal.2019.101533","article-title":"Thermophilic anaerobic conversion of raw microalgae: microbial community diversity in high solids retention systems","volume":"41","author":"Zamorano-L\u00f3pez","year":"2019","journal-title":"Algal Res"},{"key":"2025032219043372700_bib126","doi-asserted-by":"publisher","DOI":"10.1101\/2024.03.06.583742","article-title":"Microbial response to natural disturbances: rare biosphere often plays a role","volume-title":"bioRxiv","author":"Zhao","year":"2024"},{"key":"2025032219043372700_bib127","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/j.apenergy.2015.04.078","article-title":"Effect of dairy manure to switchgrass co-digestion ratio on methane production and the bacterial community in batch anaerobic digestion","volume":"151","author":"Zheng","year":"2015","journal-title":"Appl Energy"}],"container-title":["FEMS Microbiology Ecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/femsec\/advance-article-pdf\/doi\/10.1093\/femsec\/fiaf025\/62432242\/fiaf025.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/femsec\/article-pdf\/101\/4\/fiaf025\/62432242\/fiaf025.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/femsec\/article-pdf\/101\/4\/fiaf025\/62432242\/fiaf025.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,23]],"date-time":"2025-03-23T12:16:12Z","timestamp":1742732172000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/femsec\/article\/doi\/10.1093\/femsec\/fiaf025\/8082149"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,17]]},"references-count":127,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,3,18]]}},"URL":"https:\/\/doi.org\/10.1093\/femsec\/fiaf025","relation":{},"ISSN":["1574-6941"],"issn-type":[{"value":"1574-6941","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,4]]},"published":{"date-parts":[[2025,3,17]]},"article-number":"fiaf025"}}