{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T01:02:32Z","timestamp":1768006952030,"version":"3.49.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"publisher","award":["BATTIS19IO"],"award-info":[{"award-number":["BATTIS19IO"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"publisher","award":["UID\/Multi\/04046\/2013"],"award-info":[{"award-number":["UID\/Multi\/04046\/2013"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"publisher","award":["UIDP\/04046\/2020"],"award-info":[{"award-number":["UIDP\/04046\/2020"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"publisher","award":["SFRH\/BD\/114390\/2016"],"award-info":[{"award-number":["SFRH\/BD\/114390\/2016"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,8,16]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pseudomonas aeruginosa is known to exhibit considerable resistance to the antimicrobial activity of the metal-sequestering protein calprotectin (CP). In this study, we demonstrate that although CP induces zinc deficiency in P. aeruginosa, a strain unable to import zinc through the two most important metal acquisition systems, namely ZnuABC and ZrmABCD, maintains significant growth capacity in the presence of high concentrations of CP. Furthermore, we have shown that nicotianamine, a molecule structurally similar to the metallophore pseudopaline, can favor the acquisition of the metal even in the presence of CP. To gain insights into the mechanisms through which metallophores can promote zinc acquisition, we analyzed the effect of nicotianamine on the activity of the metallo-\u03b2-lactamase VIM-1. Our data suggest that metallophores released by bacteria in response to zinc deficiency can extract the protein-bound metal. The ability to interfere with the binding of metals to proteins, as well as favoring the acquisition of zinc, may contribute to increasing the resistance of P. aeruginosa to the antimicrobial action of CP.<\/jats:p>","DOI":"10.1093\/femsle\/fnac071","type":"journal-article","created":{"date-parts":[[2022,7,27]],"date-time":"2022-07-27T03:49:27Z","timestamp":1658893767000},"source":"Crossref","is-referenced-by-count":8,"title":["Zinc-binding metallophores protect<i>Pseudomonas aeruginosa<\/i>from calprotectin-mediated metal starvation"],"prefix":"10.1093","volume":"369","author":[{"given":"Serena","family":"Ammendola","sequence":"first","affiliation":[{"name":"Department of Biology, Universit\u00e0 of Rome \u2018\u2018Tor Vergata\u2019\u2019, Via della Ricerca Scientifica , 00133 Rome, Italy"}]},{"given":"Valerio","family":"Secli","sequence":"additional","affiliation":[{"name":"Department of Biology, Universit\u00e0 of Rome \u2018\u2018Tor Vergata\u2019\u2019, Via della Ricerca Scientifica , 00133 Rome, Italy"}]},{"given":"Francesca","family":"Pacello","sequence":"additional","affiliation":[{"name":"Department of Biology, Universit\u00e0 of Rome \u2018\u2018Tor Vergata\u2019\u2019, Via della Ricerca Scientifica , 00133 Rome, Italy"}]},{"given":"Maria Chiara","family":"Mastropasqua","sequence":"additional","affiliation":[{"name":"Department of Biology, Universit\u00e0 of Rome \u2018\u2018Tor Vergata\u2019\u2019, Via della Ricerca Scientifica , 00133 Rome, Italy"}]},{"given":"Mariana A","family":"Rom\u00e3o","sequence":"additional","affiliation":[{"name":"Biosystems and Integrative Sciences Institute, Faculdade de Ci\u00eancias, Universidade de Lisboa , Lisbon, Portugal"},{"name":"Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias, Universidade de Lisboa , Lisbon, Portugal"}]},{"given":"Cl\u00e1udio M","family":"Gomes","sequence":"additional","affiliation":[{"name":"Biosystems and Integrative Sciences Institute, Faculdade de 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