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We evaluated the microbiological quality of Pacific oysters (Magallana gigas) by international standard methods in four groups (each with four to five animals) acquired from supermarkets and directly from a farm producer. Most of the groups presented satisfactory microbiological quality. In two groups of oysters, \u2018questionable\u2019 or \u2018unsatisfactory\u2019 quality was observed for the coagulase-positive Staphylococcus parameter. Culture-based methods did not detect Salmonella spp. or enteropathogenic Vibrio spp., but Vibrio alginolyticus, a potential foodborne pathogen, was identified by molecular analysis. Fifty strains, belonging to 19 species, were isolated in antibiotic-supplemented media, and their antibiotic susceptibility profile was evaluated. Genes coding for \u03b2-lactamases were searched by PCR in bacteria showing resistance phenotype. Decreased susceptibility or resistance to distinct antibiotics were observed for bacteria from depurated and non-depurated oysters. The blaTEM gene was identified in Escherichia fergusonii and Shigella dysenteriae strains, which showed multidrug-resistant phenotypes. The possibility that oysters might be a source of antibiotic-resistant bacteria\/antibiotic resistance genes is of great concern and highlights the need for stricter controls and preventative measures to mitigate and counteract the dissemination of antibiotic resistance across the food chain.<\/jats:p>","DOI":"10.1093\/femsle\/fnad053","type":"journal-article","created":{"date-parts":[[2023,6,10]],"date-time":"2023-06-10T13:27:05Z","timestamp":1686403625000},"source":"Crossref","is-referenced-by-count":6,"title":["Microbiological quality, antibiotic resistant bacteria and relevant resistance genes in ready-to-eat Pacific oysters (<i>Magallana gigas<\/i>)"],"prefix":"10.1093","volume":"370","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6042-4898","authenticated-orcid":false,"given":"B\u00e1rbara","family":"Guedes","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Sciences, University of Porto , Rua do Campo Alegre s\/n, 4169-007 Porto , Portugal"}]},{"given":"Of\u00e9lia","family":"Godinho","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Sciences, University of Porto , Rua do Campo Alegre s\/n, 4169-007 Porto , Portugal"},{"name":"Interdisciplinary Centre of Marine and Environmental Research, University of Porto , Terminal de 3 Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos , Portugal"}]},{"given":"Olga Maria","family":"Lage","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Sciences, University of Porto , Rua do Campo Alegre s\/n, 4169-007 Porto , Portugal"},{"name":"Interdisciplinary Centre of Marine and Environmental Research, University of Porto , Terminal de 3 Cruzeiros do Porto de Leix\u00f5es, Avenida General Norton de Matos, S\/N, 4450-208 Matosinhos , Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0854-2771","authenticated-orcid":false,"given":"Sandra","family":"Quinteira","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Sciences, University of Porto , Rua do Campo Alegre s\/n, 4169-007 Porto , Portugal"},{"name":"BIOPOLIS\/CIBIO-InBIO \u2013 Research Centre in Biodiversity and Genetic Resources, University of Porto, Campus de Vair\u00e3o, Rua Padre Armando Quintas , n\u00b0 7, 4485\u2013661 Vair\u00e3o , Portugal"},{"name":"TOXRUN-Toxicology Research Unit, University Institute of Health Sciences, CESPU, CRL., Avenida Central de Gandra , 1317, 4585-116 Gandra PRD , Portugal"}]}],"member":"286","published-online":{"date-parts":[[2023,6,12]]},"reference":[{"key":"2024061200010668800_bib1","first-page":"D517","article-title":"CARD 2020: antibiotic resistome surveillance with the comprehensive antibiotic resistance database","volume":"48","author":"Alcock","year":"2020","journal-title":"Nucleic 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