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In this work, we present iRV973, the first reconstructed Genome-scale metabolic model (GSMM) for C. auris. The model was manually curated and experimentally validated, being able to accurately predict the specific growth rate of C. auris and the utilization of several sole carbon and nitrogen sources. The model was compared to GSMMs available for other pathogenic Candida species and exploited as a platform for cross-species comparison, aiming the analysis of their metabolic features and the identification of potential new antifungal targets common to the most prevalent pathogenic Candida species. From a metabolic point of view, we were able to identify unique enzymes in C. auris in comparison with other Candida species, which may represent unique metabolic features. Additionally, 50 enzymes were identified as potential drug targets, given their essentiality in conditions mimicking human serum, common to all four different Candida models analysed. These enzymes represent interesting drug targets for antifungal therapy, including some known targets of antifungal agents used in clinical practice, but also new potential drug targets without any human homolog or drug association in Candida species.<\/jats:p>","DOI":"10.1093\/femsyr\/foad045","type":"journal-article","created":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T21:59:19Z","timestamp":1697579959000},"source":"Crossref","is-referenced-by-count":6,"title":["Metabolic reconstruction of the human pathogen <i>Candida auris<\/i>: using a cross-species approach for drug target prediction"],"prefix":"10.1093","volume":"23","author":[{"given":"Romeu","family":"Viana","sequence":"first","affiliation":[{"name":"Department of Bioengineering, Instituto Superior T\u00e9cnico, University of Lisbon , 1049-001 Lisboa , Portugal"},{"name":"iBB - Institute for Bioengineering and Biosciences, Associate Laboratory Institute for Health and Bioeconomy - i4HB , 1049-001 Lisboa , Portugal"}]},{"given":"Tiago","family":"Carreiro","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Instituto Superior T\u00e9cnico, University of Lisbon , 1049-001 Lisboa , Portugal"},{"name":"iBB - Institute for Bioengineering and Biosciences, Associate Laboratory Institute for Health and Bioeconomy - i4HB , 1049-001 Lisboa , Portugal"}]},{"given":"Diogo","family":"Couceiro","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Instituto Superior T\u00e9cnico, University of Lisbon , 1049-001 Lisboa , Portugal"},{"name":"iBB - Institute for Bioengineering and Biosciences, Associate Laboratory Institute for Health and Bioeconomy - i4HB , 1049-001 Lisboa , Portugal"}]},{"given":"Oscar","family":"Dias","sequence":"additional","affiliation":[{"name":"CEB - Centre of Biological Engineering, Universidade do Minho , 4710-057 Braga , Portugal"}]},{"given":"Isabel","family":"Rocha","sequence":"additional","affiliation":[{"name":"ITQB Nova - Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier , 2780-157 Oeiras , Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5676-6174","authenticated-orcid":false,"given":"Miguel Cacho","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Instituto Superior T\u00e9cnico, University of Lisbon , 1049-001 Lisboa , Portugal"},{"name":"iBB - Institute for Bioengineering and Biosciences, Associate Laboratory Institute for Health and Bioeconomy - i4HB , 1049-001 Lisboa , Portugal"}]}],"member":"286","published-online":{"date-parts":[[2023,10,18]]},"reference":[{"key":"2024011612172997100_bib1","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.2147\/IDR.S249864","article-title":"Candida auris: from multidrug resistance to pan-resistant strains","volume":"13","author":"Ademe","year":"2020","journal-title":"Infect Drug Resist"},{"key":"2024011612172997100_bib2","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J Mol Biol"},{"key":"2024011612172997100_bib3","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/978-1-4939-3167-5_2","article-title":"UniProtKB\/Swiss-Prot, the manually annotated section of the UniProt KnowledgeBase: how to use the entry view","volume":"1374","author":"Boutet","year":"2016","journal-title":"Methods Mol Biol"},{"key":"2024011612172997100_bib4","doi-asserted-by":"crossref","first-page":"D471","DOI":"10.1093\/nar\/gkv1164","article-title":"The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway\/Genome databases","volume":"44","author":"Caspi","year":"2014","journal-title":"Nucleic Acids Res"},{"key":"2024011612172997100_bib5","doi-asserted-by":"crossref","first-page":"3641","DOI":"10.1128\/JCM.02248-12","article-title":"Reclassification of the Candida haemulonii complex as Candida haemulonii (C. haemulonii group I), C. duobushaemulonii sp. nov. 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