{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:08:14Z","timestamp":1760123294494,"version":"3.41.2"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T00:00:00Z","timestamp":1704844800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>White adipose tissue (WAT) controls energy storage, expenditure, and endocrine function. Rho-kinase (ROCK) is related to impaired thermogenesis, downregulation of preadipocyte differentiation, and adipokine production. Furthermore, WAT ROCK responds to metabolic stress from high-fat diets or diabetes. However, ROCK distribution in adipose depots and its response to aging and sex remain unclear. Thus, we aim to investigate ROCK function in adipose tissue of rodent and human in response to aging and sex. We observed specific differences in the ROCK1\/2 distribution in inguinal WAT (ingWAT), perigonadal WAT (pgWAT), and brown adipose tissue of male and female rodents. However, ROCK2 expression was lower in female ingWAT compared with males, a fact that was not observed in the other depots. In the pgWAT and ingWAT of male and female rodents, ROCK activity increased during development. Moreover, middle-aged female rodents and humans showed downregulation in ROCK activity after acute physical exercise. Interestingly, ROCK levels were associated with several inflammatory markers both in rats and humans WAT (Nfkb1, Tnf, Il1b, Il6, and Mcp1). Induction of cell senescence by etoposide elevates ROCK activity in human preadipocytes; however, silencing ROCK1\/2 demonstrates improvement in the inflammatory and cell senescence state. Using public databases, several pathways were strongly associated with ROCK modulation in WAT. In summary, WAT ROCK increases with development in association with inflammatory markers. Further, ROCK activity was attenuated by acute physical exercise, implicating it as a possible therapeutic target for metabolism improvement mediated by adipose tissue inflammatory state changes.<\/jats:p>","DOI":"10.1093\/gerona\/glae001","type":"journal-article","created":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T14:49:06Z","timestamp":1704898146000},"source":"Crossref","is-referenced-by-count":3,"title":["Rho-Kinase Is Differentially Expressed in the Adipose Tissue of Rodent and Human in Response to Aging, Sex, and Acute Exercise"],"prefix":"10.1093","volume":"79","author":[{"given":"Vitor Rosetto","family":"Mu\u00f1oz","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology of Exercise, University of Campinas (UNICAMP) , Limeira, S\u00e3o Paulo , Brazil"},{"name":"Section of Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School , Boston, Massachusetts , USA"}]},{"given":"Renan Fudoli Lins","family":"Vieira","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology of Exercise, University of Campinas (UNICAMP) , Limeira, S\u00e3o Paulo , Brazil"}]},{"given":"Carlos Kiyoshi","family":"Katashima","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology of Exercise, University of Campinas (UNICAMP) , Limeira, S\u00e3o Paulo , Brazil"}]},{"given":"Rafael Calais","family":"Gaspar","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology of Exercise, University of Campinas (UNICAMP) , Limeira, S\u00e3o Paulo , Brazil"}]},{"given":"Marsel","family":"Lino","sequence":"additional","affiliation":[{"name":"Section of Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School , Boston, Massachusetts , USA"}]},{"given":"Joice Cristina dos Santos","family":"Trombeta","sequence":"additional","affiliation":[{"name":"Exercise Physiology Laboratory (FISEX), Faculty of Physical Education, University of Campinas (UNICAMP) , Campinas, S\u00e3o Paulo , Brazil"}]},{"given":"Renata Garbellini","family":"Duft","sequence":"additional","affiliation":[{"name":"Exercise Physiology Laboratory (FISEX), Faculty of Physical Education, University of Campinas (UNICAMP) , Campinas, S\u00e3o Paulo , Brazil"}]},{"given":"Ana Paula","family":"Azev\u00eado Mac\u00eado","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology of Exercise, University of Campinas (UNICAMP) , Limeira, S\u00e3o Paulo , Brazil"}]},{"given":"Adelino Sanchez Ramos","family":"da Silva","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Rehabilitation and Functional Performance, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo (USP) , Ribeir\u00e3o Preto, S\u00e3o Paulo , Brazil"},{"name":"School of Physical Education and Sport of Ribeir\u00e3o Preto, University of S\u00e3o Paulo (USP) , Ribeir\u00e3o Preto, S\u00e3o Paulo , Brazil"}]},{"given":"Eduardo Rochete","family":"Ropelle","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology of Exercise, University of Campinas (UNICAMP) , Limeira, S\u00e3o Paulo , Brazil"},{"name":"OCRC\u2014Obesity and Comorbidities Research Center, University of Campinas (UNICAMP) , Campinas, S\u00e3o Paulo , Brazil"}]},{"given":"Leandro Pereira","family":"de Moura","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology of Exercise, University of Campinas (UNICAMP) , Limeira, S\u00e3o Paulo , Brazil"}]},{"given":"Dennys Esper","family":"Cintra","sequence":"additional","affiliation":[{"name":"OCRC\u2014Obesity and Comorbidities Research Center, University of Campinas (UNICAMP) , Campinas, S\u00e3o Paulo , Brazil"},{"name":"Laboratory of Nutritional Genomics, University of Campinas (UNICAMP) , Limeira, S\u00e3o Paulo , Brazil"}]},{"given":"Mara Patricia Traina","family":"Chacon-Mikahil","sequence":"additional","affiliation":[{"name":"Exercise Physiology 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