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We report on the collection, assay, and quality of aging-related biomarkers in the National Longitudinal Study of Adolescent to Adult Health (Add Health) Waves V and VI.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Staff collected blood specimens during in-home health exams, processed them locally, and shipped them to the lab for further processing, assay completion, and archiving. Analytes assessed cardiovascular disease risk, glucose homeostasis, lipids, renal function, hepatic injury, inflammation, immune system processes, and neurodegeneration. Inter-assay coefficients of variation (CVs) were estimated for sample sets. In 2% of participants, a duplicate specimen was collected within 2 weeks and assays repeated; intraclass correlation coefficients (ICCs) were calculated to assess short-term reliability.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Wave V collected blood from 92% of 5,381 participants who completed the exam (mean age 38); Wave VI collected blood from 91% of 6,073 participants (mean age 44). Reliability was excellent (ICC \u2265 0.75) and highest for hemoglobin A1c at Waves V\u2013VI and GFAP and HSV\u00a0and SARS-CoV-2 serologies\u00a0at Wave VI. Reliability was fair to good (ICC 0.40 to &amp;lt;0.75) for assays of low-abundance proteins reflecting temporally dynamic biological processes; none were poor (ICC &amp;lt; 0.40). CVs were desirable (&amp;lt;10%) for established clinical chemistry assays but less so (\u226510%) for low-abundance proteins, markers of temporally dynamic biological processes, and SARS-CoV-2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>High-quality biological data from Add Health Waves V and VI greatly expand the study\u2019s capacity to support life-course, biosocial research.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/geronb\/gbag153","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T11:48:49Z","timestamp":1785930529000},"page":"S125-S138","source":"Crossref","is-referenced-by-count":2,"title":["Laboratory methods and quality assurance in the National Longitudinal Study of Adolescent to Adult Health Waves V and VI"],"prefix":"10.1093","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9992-9597","authenticated-orcid":false,"given":"Timothy B","family":"Plante","sequence":"first","affiliation":[{"name":"Department of Medicine, Larner College of Medicine at the University of Vermont , Colchester, Vermont,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4843-3641","authenticated-orcid":false,"given":"Eric A","family":"Whitsel","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina , Chapel Hill, North Carolina,","place":["United States"]},{"name":"Department of Medicine, School of Medicine, University of North Carolina , Chapel Hill, North Carolina,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allison E","family":"Aiello","sequence":"additional","affiliation":[{"name":"Robert N. 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