{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:46:41Z","timestamp":1771980401252,"version":"3.50.1"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T00:00:00Z","timestamp":1747267200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"French Biomedicine Agency","award":["ABM 20AMP004"],"award-info":[{"award-number":["ABM 20AMP004"]}]},{"name":"University Hospital of Clermont-Ferrand"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>STUDY QUESTION<\/jats:title>\n                  <jats:p>What is the optimal stage (immature vs mature) and most efficient vitrification technique (semi-automated vs manual) to ensure the safety of the rescue-IVM (r-IVM) procedure for oocyte cryopreservation?<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>SUMMARY ANSWER<\/jats:title>\n                  <jats:p>Human oocytes should be cryopreserved after r-IVM at the mature stage (r-MII oocytes) by semi-automated or manual vitrification.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>WHAT IS KNOWN ALREADY<\/jats:title>\n                  <jats:p>r-IVM of immature oocytes may increase the number of oocytes cryopreserved for fertility preservation. However, the best stage and vitrification system (semi-automated or manual) for cryopreserving oocytes with the least possible impact on nuclear quality is unclear.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>STUDY DESIGN, SIZE, DURATION<\/jats:title>\n                  <jats:p>From January 2020 to June 2024, a prospective study was conducted on patients undergoing ICSI, including cases with at least one immature germinal vesicle (GV) stage oocyte on the day of oocyte collection, resulting in a total of 414 oocytes.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>PARTICIPANTS\/MATERIALS, SETTING, METHODS<\/jats:title>\n                  <jats:p>The study included 175 patients under 37\u2009years old with no ovulatory disorder and undergoing ICSI. A total of 414 immature oocytes were collected and divided into five groups. The control group included fresh in vitro-matured oocytes (IVM, n\u2009=\u200981). After r-IVM, oocytes were vitrified by a semi-automated technique (IVM+VITg group, n\u2009=\u200963) or a closed manual procedure (IVM+VITm group, n\u2009=\u200966). Before r-IVM, oocytes were vitrified using both techniques (VITg+IVM group, n\u2009=\u2009113 and VITm+IVM group, n\u2009=\u200991). The fresh IVM group combined IVM, IVM+VITg, and IVM+VITm groups.<\/jats:p>\n                  <jats:p>Survival rates of oocytes were evaluated 2-h post-warming. r-IVM was performed in a time-lapse incubator , allowing the assessment of r-IVM rates and maturation kinetics, including GV breakdown (GVBD) and first polar body extrusion timings. We assessed the quality of oocyte nuclear maturation through the evaluation of meiotic spindle polarity and chromosomes alignment by 3D analysis of confocal microscopy images and aneuploidy rate by array-CGH (a-CGH).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>MAIN RESULTS AND THE ROLE OF CHANCE<\/jats:title>\n                  <jats:p>The oocyte post-warming survival rate was lower when semi-automated vitrification was performed before r-IVM (50% for VITg+IVM group) compared with the three other groups (88% for IVM+VITg, 93% for IVM+VITm, and 80% for VITm+IVM, adjusted P-value\u2009&amp;lt;\u20090.001). IVM rates were not different between the three groups (fresh IVM: 80%, VITg+IVM: 80% and VITm+IVM: 69%, Pa\u2009=\u20090.131). Longer GVBD timings were observed when semi-automated vitrification was performed before r-IVM (VITg+IVM: 10.4\u2009h) compared with Fresh IVM (7.9\u2009h, Pa\u2009=\u20090.003). The percentages of oocytes with bipolar spindles or\/and aligned chromosomes were not different between the five groups. Similarly, there was no difference in aneuploidy rates (monosomy or trisomy) between the five study groups (Pa\u2009=\u20090.847). Shorter GVBD timings were observed for oocytes with a bipolar spindle or aligned chromosomes (7.8\u2009h) than for oocytes with a non-bipolar spindle or misaligned chromosomes (10.1\u2009h, Pa\u2009=\u20090.011).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>LIMITATIONS, REASONS FOR CAUTION<\/jats:title>\n                  <jats:p>The results are limited to the type of biological samples (GV-stage immature oocytes from stimulated ovaries) and the defined study population (patients \u2009&amp;lt;\u200937\u2009years old without ovulatory disorders) used in this study. Furthermore, additional research is required to evaluate the ability of oocytes to undergo successful fertilization and embryo development, as well as their capacity to result in a live birth.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>WIDER IMPLICATIONS OF THE FINDINGS<\/jats:title>\n                  <jats:p>Our findings provide reassurance regarding the use of semi-automated technique after r-IVM in clinics to optimize and standardize oocyte vitrification. The emerging field of r-IVM combined with cryopreservation by vitrification represents a promising option for fertility preservation, particularly for patients with a poor response to ovarian stimulation. Moreover, our study has revealed a previously unidentified correlation between extended GVBD timing and aberrant spindle morphology and chromosome misalignment. This provides a promising new non-invasive marker of nuclear oocyte quality for use in clinical practice.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>STUDY FUNDING\/COMPETING INTEREST(S)<\/jats:title>\n                  <jats:p>This study was funded by the French Biomedicine Agency (grant number: ABM 20AMP004) and the University Hospital of Clermont-Ferrand (France, Bourse Innovation). There are no conflicts of interest to be declared for any of the authors. There are no patents, products in development, or marketed products to declare.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>TRIAL REGISTRATION NUMBER<\/jats:title>\n                  <jats:p>ClinicalTrials.gov ID NCT03680937.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/humrep\/deaf078","type":"journal-article","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T18:32:01Z","timestamp":1747333921000},"page":"1344-1356","source":"Crossref","is-referenced-by-count":1,"title":["Analysis of maturation dynamics and oocyte nuclear quality after rescue-IVM and semi-automated vitrification"],"prefix":"10.1093","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2362-3062","authenticated-orcid":false,"given":"L","family":"Chaput","sequence":"first","affiliation":[{"name":"Assistance M\u00e9dicale \u00c0 La Procr\u00e9ation, CECOS, CHU Estaing , Clermont-Ferrand,","place":["France"]},{"name":"Universit\u00e9 Clermont Auvergne, INSERM, Imagerie Mol\u00e9culaire Et Strat\u00e9gies Th\u00e9ranostiques, UMR 1240 , Clermont-Ferrand,","place":["France"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3908-6051","authenticated-orcid":false,"given":"S","family":"Dollet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne, INSERM, Imagerie Mol\u00e9culaire Et Strat\u00e9gies Th\u00e9ranostiques, UMR 1240 , Clermont-Ferrand,","place":["France"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0460-8112","authenticated-orcid":false,"given":"E","family":"Eymard-Pierre","sequence":"additional","affiliation":[{"name":"Cytog\u00e9n\u00e9tique m\u00e9dicale, CHU Estaing , Clermont-Ferrand,","place":["France"]},{"name":"UIC CYTOMRR, GRUIC ADERGEN, CHU Clermont-Ferrand , Clermont-Ferrand,","place":["France"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3778-7161","authenticated-orcid":false,"given":"B","family":"Pereira","sequence":"additional","affiliation":[{"name":"D\u00e9l\u00e9gation \u00c0 La Recherche Clinique Et \u00c0 L\u2019Innovation, CHU Gabriel Montpied , Clermont-Ferrand,","place":["France"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4276-723X","authenticated-orcid":false,"given":"C","family":"Lucas","sequence":"additional","affiliation":[{"name":"Assistance M\u00e9dicale \u00c0 La Procr\u00e9ation, CECOS, CHU Estaing , Clermont-Ferrand,","place":["France"]}]},{"given":"A -S","family":"Gr\u00e9meau","sequence":"additional","affiliation":[{"name":"Assistance M\u00e9dicale \u00c0 La Procr\u00e9ation, CECOS, CHU Estaing , Clermont-Ferrand,","place":["France"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7050-498X","authenticated-orcid":false,"given":"A","family":"Tchirkov","sequence":"additional","affiliation":[{"name":"Cytog\u00e9n\u00e9tique m\u00e9dicale, CHU Estaing , Clermont-Ferrand,","place":["France"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0064-571X","authenticated-orcid":false,"given":"G","family":"Marteil","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Clermont Auvergne, INSERM, Imagerie Mol\u00e9culaire Et Strat\u00e9gies Th\u00e9ranostiques, UMR 1240 , Clermont-Ferrand,","place":["France"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8402-8734","authenticated-orcid":false,"given":"F","family":"Brugnon","sequence":"additional","affiliation":[{"name":"Assistance M\u00e9dicale \u00c0 La Procr\u00e9ation, CECOS, CHU Estaing , Clermont-Ferrand,","place":["France"]},{"name":"Universit\u00e9 Clermont Auvergne, INSERM, Imagerie Mol\u00e9culaire Et Strat\u00e9gies Th\u00e9ranostiques, UMR 1240 , Clermont-Ferrand,","place":["France"]}]}],"member":"286","published-online":{"date-parts":[[2025,5,15]]},"reference":[{"key":"2025070301520526100_deaf078-B1","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1017\/S0967199413000233","article-title":"Developmental potential of in vitro or in vivo matured oocytes","volume":"23","author":"Alcoba","year":"2015","journal-title":"Zygote Camb Engl"},{"key":"2025070301520526100_deaf078-B2","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1186\/s13048-022-01064-3","article-title":"Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses","volume":"15","author":"Barberet","year":"2022","journal-title":"J Ovarian Res"},{"key":"2025070301520526100_deaf078-B3","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.rbmo.2009.12.009","article-title":"Contribution of in-vitro maturation in ovarian stimulation cycles of poor-responder patients","volume":"20","author":"Braga","year":"2010","journal-title":"Reprod Biomed Online"},{"key":"2025070301520526100_deaf078-B4","first-page":"70","article-title":"The first livebirth using warmed oocytes by a semi-automated vitrification procedure","volume":"22","author":"Brunetti","year":"2021","journal-title":"J Reprod Infertil"},{"key":"2025070301520526100_deaf078-B5","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/S1472-6483(10)60170-8","article-title":"Cryopreservation of immature and in-vitro matured human oocytes by vitrification","volume":"19","author":"Cao","year":"2009","journal-title":"Reprod Biomed Online"},{"key":"2025070301520526100_deaf078-B6","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1093\/biolre\/ioab239","article-title":"The effects of vitrification on oocyte quality","volume":"106","author":"Chang","year":"2022","journal-title":"Biol Reprod"},{"key":"2025070301520526100_deaf078-B7","doi-asserted-by":"crossref","first-page":"2222","DOI":"10.1093\/humrep\/dey321","article-title":"Elective and onco-fertility preservation: factors related to IVF outcomes","volume":"33","author":"Cobo","year":"2018","journal-title":"Hum Reprod Oxf Engl"},{"key":"2025070301520526100_deaf078-B8","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/j.fertnstert.2015.11.027","article-title":"Oocyte vitrification as an efficient option for elective fertility preservation","volume":"105","author":"Cobo","year":"2016","journal-title":"Fertil Steril"},{"key":"2025070301520526100_deaf078-B9","doi-asserted-by":"crossref","first-page":"2239","DOI":"10.1093\/humrep\/deq146","article-title":"Use of cryo-banked oocytes in an ovum donation programme: a prospective, randomized, controlled, clinical trial","volume":"25","author":"Cobo","year":"2010","journal-title":"Hum Reprod Oxf Engl"},{"key":"2025070301520526100_deaf078-B10","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.jogoh.2019.05.012","article-title":"The first report of pregnancies following blastocyst automated vitrification in Europe","volume":"48","author":"Dal Canto","year":"2019","journal-title":"J Gynecol Obstet Hum Reprod"},{"key":"2025070301520526100_deaf078-B11","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.rbmo.2018.01.016","article-title":"Prospective assessment of follicular growth and the oocyte cohort after ovarian stimulation for fertility preservation in 90 cancer patients versus 180 matched controls","volume":"36","author":"Decanter","year":"2018","journal-title":"Reprod Biomed Online"},{"key":"2025070301520526100_deaf078-B12","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1016\/j.fertnstert.2009.01.133","article-title":"Learning curve of vitrification assessed by cumulative summation test for learning curve (LC-CUSUM)","volume":"92","author":"Dessolle","year":"2009","journal-title":"Fertil Steril"},{"key":"2025070301520526100_deaf078-B13","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1016\/j.fertnstert.2012.07.1116","article-title":"The dynamics of in vitro maturation of germinal vesicle oocytes","volume":"98","author":"Escrich","year":"2012","journal-title":"Fertil Steril"},{"key":"2025070301520526100_deaf078-B14","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1002\/ijgo.13702","article-title":"Female fertility preservation: impact of cancer on ovarian function and oocyte quality","volume":"156","author":"Fabiani","year":"2022","journal-title":"Int J Gynecol Obstet"},{"key":"2025070301520526100_deaf078-B15","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1007\/s10815-012-9751-9","article-title":"In-vitro maturation of human oocytes: before or after vitrification?","volume":"29","author":"Fasano","year":"2012","journal-title":"J Assist Reprod Genet"},{"key":"2025070301520526100_deaf078-B16","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.fertnstert.2011.10.014","article-title":"Ovarian response to stimulation for fertility preservation in women with malignant disease: a systematic review and meta-analysis","volume":"97","author":"Friedler","year":"2012","journal-title":"Fertil Steril"},{"key":"2025070301520526100_deaf078-B17","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1002\/mrd.1080400206","article-title":"In vitro viability and ultrastructural changes in bovine oocytes treated with a vitrification solution","volume":"40","author":"Fuku","year":"1995","journal-title":"Mol Reprod Dev"},{"key":"2025070301520526100_deaf078-B18","doi-asserted-by":"crossref","first-page":"3213","DOI":"10.1007\/s10815-021-02346-3","article-title":"Semi-automated versus manual embryo vitrification: inter-operator variability, time-saving, and clinical outcomes","volume":"38","author":"Gatimel","year":"2021","journal-title":"J Assist Reprod Genet"},{"key":"2025070301520526100_deaf078-B19","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.3390\/jcm11061703","article-title":"Exploring the developmental potential of human germinal vesicle oocytes aiming at fertility preservation: can we increase the yields of competent oocytes through IVM combined with vitrification?","volume":"11","author":"Hao","year":"2022","journal-title":"J Clin Med"},{"key":"2025070301520526100_deaf078-B20","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1017\/S0967199416000290","article-title":"Early germinal vesicle breakdown is a predictor of high preimplantation developmental competent oocytes in mice","volume":"25","author":"Higaki","year":"2017","journal-title":"Zygote Camb Engl"},{"key":"2025070301520526100_deaf078-B21","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1126\/science.aaa9529","article-title":"Human oocytes. Error-prone chromosome-mediated spindle assembly favors chromosome segregation defects in human oocytes","volume":"348","author":"Holubcov\u00e1","year":"2015","journal-title":"Science"},{"key":"2025070301520526100_deaf078-B22","doi-asserted-by":"crossref","first-page":"1991","DOI":"10.1093\/humrep\/dem124","article-title":"Optimal ICSI timing after the first polar body extrusion in in vitro matured human oocytes","volume":"22","author":"Hyun","year":"2007","journal-title":"Hum Reprod Oxf Engl"},{"key":"2025070301520526100_deaf078-B23","first-page":"85","article-title":"Vitrification of human germinal vesicle oocytes: before or after in vitro maturation?","volume":"11","author":"Kasapi","year":"2017","journal-title":"Int J Fertil Steril"},{"key":"2025070301520526100_deaf078-B24","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1007\/s10815-017-1005-4","article-title":"Vitrification of human immature oocytes before and after in vitro maturation: a review","volume":"34","author":"Khalili","year":"2017","journal-title":"J Assist Reprod Genet"},{"key":"2025070301520526100_deaf078-B25","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1016\/S0015-0282(00)01617-4","article-title":"In vitro maturation, fertilization, and development of human germinal vesicle oocytes collected from stimulated cycles","volume":"74","author":"Kim","year":"2000","journal-title":"Fertil Steril"},{"key":"2025070301520526100_deaf078-B26","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s12020-015-0744-1","article-title":"Rescue in vitro maturation (IVM) of immature oocytes in stimulated cycles in women with low functional ovarian reserve (LFOR)","volume":"52","author":"Lee","year":"2016","journal-title":"Endocrine"},{"key":"2025070301520526100_deaf078-B27","doi-asserted-by":"crossref","first-page":"181","DOI":"10.3349\/ymj.2012.53.1.181","article-title":"Oocyte maturity in relation to woman\u2019s age in in vitro fertilization cycles stimulated by single regimen","volume":"53","author":"Lee","year":"2012","journal-title":"Yonsei Med J"},{"key":"2025070301520526100_deaf078-B28","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1159\/000448143","article-title":"Ultrastructural changes and methylation of human oocytes vitrified at the germinal vesicle stage and matured in vitro after thawing","volume":"82","author":"Liu","year":"2017","journal-title":"Gynecol Obstet Invest"},{"key":"2025070301520526100_deaf078-B29","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/j.fertnstert.2006.02.083","article-title":"First meiosis errors in immature oocytes generated by stimulated cycles","volume":"86","author":"Magli","year":"2006","journal-title":"Fertil Steril"},{"key":"2025070301520526100_deaf078-B30","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.rbmo.2018.10.002","article-title":"Morphokinetic characteristics of embryos derived from in-vitro-matured oocytes and their in-vivo-matured siblings after ovarian stimulation","volume":"38","author":"Margalit","year":"2019","journal-title":"Reprod Biomed Online"},{"key":"2025070301520526100_deaf078-B31","doi-asserted-by":"crossref","first-page":"2625","DOI":"10.1007\/s43032-024-01596-7","article-title":"Vitrification of human oocytes before or after rescue-IVM does not impair maturation kinetics but induces meiotic spindle alterations","volume":"31","author":"Marteil","year":"2024","journal-title":"Reprod Sci"},{"key":"2025070301520526100_deaf078-B32","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1002\/rmb2.12320","article-title":"Equivalent clinical outcome after vitrified-thawed blastocyst transfer using semi-automated embryo vitrification system compared with manual vitrification method","volume":"19","author":"Miwa","year":"2020","journal-title":"Reprod Med Biol"},{"key":"2025070301520526100_deaf078-B33","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s12958-016-0161-1","article-title":"Osmotic-shock produced by vitrification solutions improves immature human oocytes in vitro maturation","volume":"14","author":"Molina","year":"2016","journal-title":"Reprod Biol Endocrinol"},{"key":"2025070301520526100_deaf078-B34","first-page":"209","article-title":"Maturation capacity, morphology and morphometric assessment of human immature oocytes after vitrification and in-vitro maturation","volume":"9","author":"Nazari","year":"2011","journal-title":"Iran J Reprod Med"},{"key":"2025070301520526100_deaf078-B35","doi-asserted-by":"crossref","first-page":"e118","DOI":"10.1136\/jmg.2004.022400","article-title":"Rapid detection of CFTR gene rearrangements impacts on genetic counselling in cystic fibrosis","volume":"41","author":"Niel","year":"2004","journal-title":"J Med Genet"},{"key":"2025070301520526100_deaf078-B36","doi-asserted-by":"crossref","first-page":"2651","DOI":"10.3390\/jcm13092651","article-title":"The effect of open and closed oocyte vitrification systems on embryo development: a systematic review and network meta-analysis","volume":"13","author":"Pantos","year":"2024","journal-title":"J Clin Med"},{"key":"2025070301520526100_deaf078-B37","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.3390\/ijms22031125","article-title":"Impact of maturation and vitrification time of human GV oocytes on the metaphase plate configuration","volume":"22","author":"Peinado","year":"2021","journal-title":"Int J Mol Sci"},{"key":"2025070301520526100_deaf078-B38","doi-asserted-by":"crossref","first-page":"1047571","DOI":"10.3389\/fendo.2022.1047571","article-title":"Rescue in vitro maturation may increase the pregnancy outcomes among women undergoing intracytoplasmic sperm injection","volume":"13","author":"Qin","year":"2022","journal-title":"Front Endocrinol"},{"key":"2025070301520526100_deaf078-B39","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1007\/s10815-010-9416-5","article-title":"Extended in vitro maturation of immature oocytes from stimulated cycles: an analysis of fertilization potential, embryo development, and reproductive outcomes","volume":"27","author":"Reichman","year":"2010","journal-title":"J Assist Reprod Genet"},{"key":"2025070301520526100_deaf078-B40","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1093\/humrep\/dep346","article-title":"Embryo development of fresh \u201cversus\u201d vitrified metaphase II oocytes after ICSI: a prospective randomized sibling-oocyte study","volume":"25","author":"Rienzi","year":"2010","journal-title":"Hum Reprod Oxf Engl"},{"key":"2025070301520526100_deaf078-B41","doi-asserted-by":"crossref","first-page":"2431","DOI":"10.1093\/humrep\/deu214","article-title":"Embryo vitrification using a novel semi-automated closed system yields in vitro outcomes equivalent to the manual Cryotop method","volume":"29","author":"Roy","year":"2014","journal-title":"Hum Reprod Oxf Engl"},{"key":"2025070301520526100_deaf078-B43","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.ejogrb.2012.11.006","article-title":"The effect of vitrification on ultrastructure of human in vitro matured germinal vesicle oocytes","volume":"167","author":"Shahedi","year":"2013","journal-title":"Eur J Obstet Gynecol Reprod Biol"},{"key":"2025070301520526100_deaf078-B44","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1016\/j.fertnstert.2023.05.163","article-title":"The developmental potential of mature oocytes derived from rescue in vitro maturation (IVM)","volume":"120","author":"Shani","year":"2023","journal-title":"Fertil Steril"},{"key":"2025070301520526100_deaf078-B45","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1159\/000445564","article-title":"Effects of vitrification on outcomes of in vivo-mature, in vitro-mature and immature human oocytes","volume":"38","author":"Song","year":"2016","journal-title":"Cell Physiol Biochem Int J Exp Cell Physiol Biochem Pharmacol"},{"key":"2025070301520526100_deaf078-B46","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1093\/humrep\/deh236","article-title":"The outcome of ICSI of immature MI oocytes and rescued in vitro matured MII oocytes","volume":"19","author":"Strassburger","year":"2004","journal-title":"Hum Reprod"},{"key":"2025070301520526100_deaf078-B47","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1016\/j.fertnstert.2015.12.018","article-title":"Is oocyte meiotic spindle morphology associated with embryo ploidy? A prospective cohort study","volume":"105","author":"Tilia","year":"2016","journal-title":"Fertil Steril"},{"key":"2025070301520526100_deaf078-B48","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/S0015-0282(16)56891-5","article-title":"In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients","volume":"62","author":"Trounson","year":"1994","journal-title":"Fertil Steril"},{"key":"2025070301520526100_deaf078-B49","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.ejogrb.2007.03.013","article-title":"How safe is germinal vesicle stage oocyte rescue? Aneuploidy analysis of in vitro matured oocytes","volume":"134","author":"Vlaisavljevi\u0107","year":"2007","journal-title":"Eur J Obstet Gynecol Reprod Biol"},{"key":"2025070301520526100_deaf078-B50","doi-asserted-by":"crossref","first-page":"6187","DOI":"10.1007\/s11033-011-1436-4","article-title":"Comparison of aneuploidy frequencies between in vitro matured and unstimulated cycles oocytes by metaphase comparative genomic hybridization (mCGH)","volume":"39","author":"Yakut","year":"2012","journal-title":"Mol Biol Rep"},{"key":"2025070301520526100_deaf078-B52","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.fertnstert.2011.04.077","article-title":"Optimal timing of oocyte maturation and its relationship with the spindle assembly and developmental competence of in vitro matured human oocytes","volume":"96","author":"Yu","year":"2011","journal-title":"Fertil Steril"},{"key":"2025070301520526100_deaf078-B53","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.fertnstert.2023.10.026","article-title":"Rescue in vitro maturation and the transfer of a euploid blastocyst provided improved chances for patients with poor prognosis to conceive","volume":"121","author":"Yuan","year":"2024","journal-title":"Fertil Steril"}],"container-title":["Human Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/humrep\/article-pdf\/40\/7\/1344\/63198567\/deaf078.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/humrep\/article-pdf\/40\/7\/1344\/63198567\/deaf078.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T05:52:19Z","timestamp":1751521939000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/humrep\/article\/40\/7\/1344\/8133169"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,15]]},"references-count":51,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,5,15]]},"published-print":{"date-parts":[[2025,7,1]]}},"URL":"https:\/\/doi.org\/10.1093\/humrep\/deaf078","relation":{},"ISSN":["0268-1161","1460-2350"],"issn-type":[{"value":"0268-1161","type":"print"},{"value":"1460-2350","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,7]]},"published":{"date-parts":[[2025,5,15]]}}}