{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T06:28:11Z","timestamp":1772000891812,"version":"3.50.1"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Barrento, S., Marques, A., Vaz-Pires, P., and Nunes, M. L. 2010. Live shipment of immersed crabs Cancer pagurus from England to Portugal and recovery in stocking tanks: stress parameter characterization. \u2013 ICES Journal of Marine Science, 67: 435\u2013443. Cancer pagurus is commercially one of the most important crustaceans exploited in the UK and Ireland, but the main markets are in southern Europe, to where live edible crabs are transported. In this study, potential stressors during the live trade chain from England to Portugal were identified and related to changes in haemolymph parameters. Before their live transport, 60 crabs were tagged, their vigour was assessed, and their haemolymph was sampled; 30 crabs were placed in the bottom of a vivier-truck tank and the balance at the top (1 kg crab l\u22121 in a total of 700 kg). The sampling procedure was repeated after immersed live transport (58 h), and during subsequent recovery (6, 24, 48, 72, and 96 h) in seawater at Portuguese storage facilities. Haemolymph parameters included pH, d-glucose, l-lactate, and haemocyanine. Cumulative mortalities at the end of the experiment (96 h in recovery tanks) of bottom and top crabs were 8.9 and 10.7%, respectively. Vigour assessment predicted crab mortality well. The main stressors identified were poor handling; air exposure during unloading, and deficient transport conditions. d-Glucose and l-lactate increased during transport with acidification of the haemolymph. Concentration of l-lactate reached control levels after 24 h of recovery, but haemolymph remained acidic and hyperglycaemic even after 96 h. The transport conditions promoted anaerobiosis, so alternatives need to be considered.<\/jats:p>","DOI":"10.1093\/icesjms\/fsp268","type":"journal-article","created":{"date-parts":[[2009,12,11]],"date-time":"2009-12-11T01:21:09Z","timestamp":1260494469000},"page":"435-443","source":"Crossref","is-referenced-by-count":33,"title":["Live shipment of immersed crabs Cancer pagurus from England to Portugal and recovery in stocking tanks: stress parameter characterization"],"prefix":"10.1093","volume":"67","author":[{"given":"Sara","family":"Barrento","sequence":"first","affiliation":[{"name":"Research Unit of Upgrading of Fishery and Aquaculture Products (U-VPPA), National Institute of Biological Resources (INRB-IPIMAR), Avenida de Bras\u00edlia, Alg\u00e9s, 1449-006 Lisboa, Portugal"},{"name":"Institute of Biomedical Sciences Abel Salazar (ICBAS-UP), University of Porto, Largo Professor Abel Salazar 2, 4099-003 Porto, Portugal"}]},{"given":"Ant\u00f3nio","family":"Marques","sequence":"additional","affiliation":[{"name":"Research Unit of Upgrading of Fishery and Aquaculture Products (U-VPPA), National Institute of Biological Resources (INRB-IPIMAR), Avenida de Bras\u00edlia, Alg\u00e9s, 1449-006 Lisboa, Portugal"}]},{"given":"Paulo","family":"Vaz-Pires","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences Abel Salazar (ICBAS-UP), University of Porto, Largo Professor Abel Salazar 2, 4099-003 Porto, Portugal"},{"name":"Centre of Marine and Environmental Research, University of Porto (CIIMAR-UP), Rua dos Bragas 289, 4050-123 Porto, Portugal"}]},{"given":"Maria Leonor","family":"Nunes","sequence":"additional","affiliation":[{"name":"Research Unit of Upgrading of Fishery and Aquaculture Products (U-VPPA), National Institute of Biological Resources (INRB-IPIMAR), Avenida de Bras\u00edlia, Alg\u00e9s, 1449-006 Lisboa, Portugal"}]}],"member":"286","published-online":{"date-parts":[[2009,12,10]]},"reference":[{"key":"2019081212154620800_FSP268C1","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1093\/icesjms\/fsn037","article-title":"The trade of live crustaceans in Portugal: space for technological improvements","volume":"65","author":"Barrento","year":"2008","journal-title":"ICES Journal of Marine Science"},{"key":"2019081212154620800_FSP268C2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/0959-8030(91)90019-G","article-title":"Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids","volume":"1","author":"Barton","year":"1991","journal-title":"Annual Review of Fish Diseases"},{"key":"2019081212154620800_FSP268C3","doi-asserted-by":"crossref","first-page":"135","DOI":"10.3354\/dao01977","article-title":"Effects of emersion-induced hypoxia on some haemolymph constituents of Nephrops norvegicus","volume":"82","author":"Bernasconi","year":"2008","journal-title":"Diseases of Aquatic Organisms"},{"key":"2019081212154620800_FSP268C4","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1098\/rspb.2001.1752","article-title":"Decision rules, energy metabolism and vigour of hermit-crab fights","volume":"268","author":"Briffa","year":"2001","journal-title":"Proceedings of the Royal Society of London, Series B: Biological Sciences"},{"key":"2019081212154620800_FSP268C5","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1006\/gcen.1998.7120","article-title":"Quantification of crustacean hyperglycemic hormone by ELISA in hemolymph of the lobster, Homarus americanus, following various stresses","volume":"111","author":"Chang","year":"1998","journal-title":"General and Comparative Endocrinology"},{"key":"2019081212154620800_FSP268C6","first-page":"65","article-title":"Stocking and transportation of live crustaceans","author":"Chartois","year":"1994"},{"key":"2019081212154620800_FSP268C7","first-page":"9","article-title":"Effect of long-haul international transport on lobster hemolymph constituents and nitrogen metabolism","volume-title":"Marketing and Shipping Live Aquatic Products. Proceedings of the Second International Conference and Exhibition, Seattle, WA, November 1999","author":"Danford","year":"2001"},{"key":"2019081212154620800_FSP268C8","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S1095-6433(00)00253-1","article-title":"Nitrogen excretion and changes in haemolymph components during emersion of the subtidal spider crab Maia squinado (L.)","volume":"127","author":"Durand","year":"2000","journal-title":"Comparative Biochemistry and Physiology A"},{"key":"2019081212154620800_FSP268C9","doi-asserted-by":"crossref","first-page":"2515","DOI":"10.1242\/jeb.201.17.2515","article-title":"Nitrogen metabolism of two portunid crabs, Carcinus maenas and Necora puber during prolonged air exposure and subsequent recovery: a comparative study","volume":"201","author":"Durand","year":"1998","journal-title":"Journal of Experimental Biology"},{"key":"2019081212154620800_FSP268C10","article-title":"The Edible Crab and its Fishery in British Waters","author":"Edwards","year":"1979"},{"key":"2019081212154620800_FSP268C11","author":"Eurostat","year":"2009"},{"key":"2019081212154620800_FSP268C12","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.cbpa.2006.01.010","article-title":"Effect of holding duration on the immune system of western rock lobster, Panulirus cygnus","volume":"143","author":"Fotedar","year":"2006","journal-title":"Comparative Biochemistry and Physiology A"},{"key":"2019081212154620800_FSP268C13","first-page":"271","article-title":"Using HACCP principles and physiological studies to improve marketing practices for live crustaceans","volume-title":"Marketing and Shipping Live Aquatic Products. Proceedings of the Second International Conference and Exhibition, Seattle, WA, November 1999","author":"G\u00f3mez-Jim\u00e9nez","year":"2001"},{"key":"2019081212154620800_FSP268C14","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0300-9629(90)90721-4","article-title":"Aspects of haemolymph physiology and ammonia excretion in Nephrops norvegicus under hypoxia","volume":"97","author":"Hagerman","year":"1990","journal-title":"Comparative Biochemistry and Physiology A"},{"key":"2019081212154620800_FSP268C15","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.jembe.2004.12.029","article-title":"Physiological changes in the Norway lobster Nephrops norvegicus (L.) escaping and discarded from commercial trawls on the West Coast of Scotland. 1. Body fluids volumes and haemolymph composition after capture and during recovery","volume":"320","author":"Harris","year":"2005","journal-title":"Journal of Experimental Marine Biology and Ecology"},{"key":"2019081212154620800_FSP268C16","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.jembe.2004.12.028","article-title":"Physiological changes in the Norway lobster Nephrops norvegicus (L.) escaping and discarded from commercial trawls on the West Coast of Scotland. 2. Disturbances in haemolymph respiratory gases, tissue metabolites and swimming performance after capture and during recovery","volume":"320","author":"Harris","year":"2005","journal-title":"Journal of Experimental Marine Biology and Ecology"},{"key":"2019081212154620800_FSP268C17","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/0022-0981(95)00118-2","article-title":"The ecological consequences of limb damage and loss in decapod crustaceans: a review and prospectus","volume":"193","author":"Juanes","year":"1995","journal-title":"Journal of Experimental Marine Biology and Ecology"},{"key":"2019081212154620800_FSP268C18","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1071\/MF97216","article-title":"Total and differential haemocyte counts in western rock lobsters (Panulirus cygnus George) under post-harvest stress","volume":"48","author":"Jussila","year":"1997","journal-title":"Marine and Freshwater Research"},{"key":"2019081212154620800_FSP268C19","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.aquaculture.2008.03.011","article-title":"Stress effect of two different transport systems on the physiological profiles of the crab Cancer pagurus","volume":"278","author":"Lorenzon","year":"2008","journal-title":"Aquaculture"},{"key":"2019081212154620800_FSP268C20","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.cbpa.2006.11.028","article-title":"Stress effect of different temperatures and air exposure during transport on physiological profiles in the American lobster Homarus americanus","volume":"147","author":"Lorenzon","year":"2007","journal-title":"Comparative Biochemistry and Physiology A"},{"key":"2019081212154620800_FSP268C21","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.jembe.2009.04.004","article-title":"Recovery by the Norway lobster Nephrops norvegicus (L.) from the physiological stresses of trawling: influence of season and live-storage position","volume":"373","author":"Lund","year":"2009","journal-title":"Journal of Experimental Marine Biology and Ecology"},{"key":"2019081212154620800_FSP268C22","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.anbehav.2004.06.017","article-title":"Changes in blood D-glucose and L-lactate levels of male fiddler crabs: effects of aggression and claw waving","volume":"69","author":"Matsumasa","year":"2005","journal-title":"Animal Behaviour"},{"key":"2019081212154620800_FSP268C23","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1071\/MF97069","article-title":"Haemolymph chemistry of tropical rock lobsters (Panulirus ornatus) brought onto a mother ship from a catching dinghy in Torres Strait","volume":"48","author":"Paterson","year":"1997","journal-title":"Marine and Freshwater Research"},{"key":"2019081212154620800_FSP268C24","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1071\/MF97137","article-title":"Stress indicators in marine decapod crustaceans, with particular reference to the grading of western rock lobster (Panulirus cygnus) during commercial handling","volume":"48","author":"Paterson","year":"1997","journal-title":"Marine and Freshwater Research"},{"key":"2019081212154620800_FSP268C25","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.jembe.2005.07.015","article-title":"Morbidity and mortality in Norway lobsters, Nephrops norvegicus: physiological, immunological and pathological effects of air exposure","volume":"328","author":"Ridgway","year":"2006","journal-title":"Journal of Experimental Marine Biology and Ecology"},{"key":"2019081212154620800_FSP268C26","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1071\/MF97203","article-title":"Relative influence of environmental factors and processing techniques on Panulirus cygnus morbidity and mortality during simulated live shipments","volume":"48","author":"Spanoghe","year":"1997","journal-title":"Marine and Freshwater Research"},{"key":"2019081212154620800_FSP268C27","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/BF01344278","article-title":"Effect of air exposure on concentrations of selected metabolites in the blood of the Norwegian lobster Nephrops norvegicus (Crustacea: Nephropidae)","volume":"105","author":"Spicer","year":"1990","journal-title":"Marine Biology"},{"key":"2019081212154620800_FSP268C28","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1007\/BF00393095","article-title":"Metabolic responses of the prawns Palaemon elegans and P. serratus (Crustacea: Decapoda) to acute hypoxia and anoxia","volume":"95","author":"Taylor","year":"1987","journal-title":"Marine Biology"},{"key":"2019081212154620800_FSP268C29","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1071\/MF97197","article-title":"Physiology and live transport of lobsters: report from a workshop","volume":"48","author":"Taylor","year":"1997","journal-title":"Marine and Freshwater Research"},{"key":"2019081212154620800_FSP268C30","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1071\/MF97071","article-title":"Respiratory responses to air-exposure in the southern rock lobster, Jasus edwardsii (Hutton) (Decapoda: Palinuridae)","volume":"48","author":"Taylor","year":"1997","journal-title":"Marine and Freshwater Research"},{"key":"2019081212154620800_FSP268C31","first-page":"24","article-title":"Live handling and transport of crustacean shellfish: an investigation of mortalities","author":"Uglow","year":"1986"},{"key":"2019081212154620800_FSP268C32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0022-0981(99)00004-0","article-title":"An in situ study of respiratory variables in three British sublittoral crabs with different routine rates of activity","volume":"239","author":"Watt","year":"1999","journal-title":"Journal of Experimental Marine Biology and Ecology"},{"key":"2019081212154620800_FSP268C33","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.1242\/jeb.199.7.1579","article-title":"Measurement of crustacean hyperglycaemic hormone levels in the edible crab Cancer pagurus during emersion stress","volume":"199","author":"Webster","year":"1996","journal-title":"Journal of Experimental Biology"}],"container-title":["ICES Journal of Marine Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/icesjms\/article-pdf\/67\/3\/435\/29135680\/fsp268.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,15]],"date-time":"2021-10-15T22:53:47Z","timestamp":1634338427000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/icesjms\/article\/67\/3\/435\/733388"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12,10]]},"references-count":33,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2009,12,10]]},"published-print":{"date-parts":[[2010,4,1]]}},"URL":"https:\/\/doi.org\/10.1093\/icesjms\/fsp268","relation":{},"ISSN":["1095-9289","1054-3139"],"issn-type":[{"value":"1095-9289","type":"electronic"},{"value":"1054-3139","type":"print"}],"subject":[],"published-other":{"date-parts":[[2010,4]]},"published":{"date-parts":[[2009,12,10]]}}}