{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T20:16:36Z","timestamp":1770495396574,"version":"3.49.0"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T00:00:00Z","timestamp":1715212800000},"content-version":"vor","delay-in-days":129,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Norwegian Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]},{"name":"AQUAeD"},{"DOI":"10.13039\/501100005416","name":"Research Council of Norway","doi-asserted-by":"publisher","award":["320076"],"award-info":[{"award-number":["320076"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The performance of sequence variant resolution analytic tools for metabarcoding has not yet been adequately benchmarked for high-diversity environmental samples. We therefore evaluated the sequence variant tools DADA2, Deblur, Swarm, and UNOISE, using high-diversity seafloor samples, resulting in comparisons of 1800 sequence variant tables. The evaluation was based on 30 sediment grab samples, for which 3 replica samples were collected. Each replica sample was extracted using 5 common DNA extraction kits, resulting in 450 DNA extracts which were 16S rRNA gene sequenced (V3\u2013V4), using Illumina. Assessments included variation across replica samples, extraction kits, and denoising methods, in addition to applying prior knowledge about alpha diversity correlations toward the cosmopolitan marine archaeon Nitrosopumilus with high diversity and the sulfide oxidizing Sulfurovum with low diversity. DADA2 displayed the highest variance between replicates (Manhattan distance 1.14), while Swarm showed the lowest variance (Manhattan distance 0.93). For the analysis based on prior biological knowledge, UNOISE displayed the highest alpha diversity (Simpson\u2019s D) correlation toward Nitrosopumilus (Spearman rho\u2009=\u20090.85), while DADA2 showed the lowest (Spearman rho\u2009=\u20090.10). Deblur completely eliminated Nitrosopumilus from the dataset. For Sulfurovum, on the other hand, all the methods showed comparable results. In conclusion, our evaluations show that Swarm and UNOISE performed better than DADA2 and Deblur for high-diversity seafloor samples.<\/jats:p>","DOI":"10.1093\/ismeco\/ycae071","type":"journal-article","created":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T17:17:55Z","timestamp":1715275075000},"source":"Crossref","is-referenced-by-count":8,"title":["Swarm and UNOISE outperform DADA2 and Deblur for denoising high-diversity marine seafloor samples"],"prefix":"10.1093","volume":"4","author":[{"given":"Tonje","family":"Nilsen","sequence":"first","affiliation":[{"name":"Faculty of Chemistry , Biotechnology and Food Science (KBM), , Chr. M. Falsensvei 18, Biotechnology Building, 1432 \u00c5s , Norway"},{"name":"Norwegian University of Life Sciences (NMBU) , Biotechnology and Food Science (KBM), , Chr. M. Falsensvei 18, Biotechnology Building, 1432 \u00c5s , Norway"}]},{"given":"Lars-Gustav","family":"Snipen","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry , Biotechnology and Food Science (KBM), , Chr. M. Falsensvei 18, Biotechnology Building, 1432 \u00c5s , Norway"},{"name":"Norwegian University of Life Sciences (NMBU) , Biotechnology and Food Science (KBM), , Chr. M. Falsensvei 18, Biotechnology Building, 1432 \u00c5s , Norway"}]},{"given":"Inga Leena","family":"Angell","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry , Biotechnology and Food Science (KBM), , Chr. M. Falsensvei 18, Biotechnology Building, 1432 \u00c5s , Norway"},{"name":"Norwegian University of Life Sciences (NMBU) , Biotechnology and Food Science (KBM), , Chr. M. Falsensvei 18, Biotechnology Building, 1432 \u00c5s , Norway"}]},{"given":"Nigel Brian","family":"Keeley","sequence":"additional","affiliation":[{"name":"Institute of Marine Research , Dept. Tromso, P.O. Box 6606, Stakkevollan, 9296 Troms\u00f8 , Norway"}]},{"given":"Sanna","family":"Majaneva","sequence":"additional","affiliation":[{"name":"Akvaplan-niva , Framsenteret, P.O. Box 6606, Stakkevollan, 9296 Troms\u00f8 , Norway"}]},{"given":"Ragnhild","family":"Pettersen","sequence":"additional","affiliation":[{"name":"Akvaplan-niva , Framsenteret, P.O. Box 6606, Stakkevollan, 9296 Troms\u00f8 , Norway"}]},{"given":"Knut","family":"Rudi","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry , Biotechnology and Food Science (KBM), Norwegian University of Life Sciences (NMBU), Chr. M. 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