{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T00:15:43Z","timestamp":1769818543034,"version":"3.49.0"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2020,5,17]],"date-time":"2020-05-17T00:00:00Z","timestamp":1589673600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Associate Laboratory for Green Chemistry-LAQV"},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006129","name":"FCT","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006111","name":"MCTES","doi-asserted-by":"publisher","award":["UID\/QUI\/50006\/2019"],"award-info":[{"award-number":["UID\/QUI\/50006\/2019"]}],"id":[{"id":"10.13039\/501100006111","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006129","name":"FCT","doi-asserted-by":"publisher","award":["SFRH\/BD\/137947\/2018"],"award-info":[{"award-number":["SFRH\/BD\/137947\/2018"]}],"id":[{"id":"10.13039\/100006129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>The aim of this study was to evaluate the efficacy of dalbavancin against MRSA biofilm-related infection in orthopaedic implants in vivo.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>One MRSA strain isolated from human osteomyelitis was used to promote biofilm formation on the surface of screws. The implants were inserted in the proximal tibia under general anaesthesia. Thirty-nine Wistar rats were divided into three groups [control group (no treatment), Group 1 (7\u2009days of treatment) and Group 2 (14\u2009days of treatment)]; both treatment groups were administered dalbavancin intraperitoneally and euthanized after treatment. cfu of bacteria present in both the tibia and the implant were quantified. The infection severity was assessed by histopathology and scored from 0 (no infection) to 4 (severe infection).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The high number of cfu\/g and cfu\/mL present in the control group indicated a well-established infection. There was a significant reduction in cfu in rats treated with dalbavancin both in the tibia (2.8\u205f\u00d7\u205f105\u2009cfu\/g) and the implant (1.1\u205f\u00d7\u205f106\u2009cfu\/mL) in Group 1 (1.8\u205f\u00d7\u205f103\u2009cfu\/g and 2.4\u205f\u00d7\u205f105\u2009cfu\/mL, respectively) and in Group 2 (8.2\u2009cfu\/g and 8.2\u205f\u00d7\u205f103\u2009cfu\/mL, respectively). Most animals from the control group presented an infection scored as 3 (severe). At the end of the experiment, most rats from Groups 1 and 2 presented an infection scored as 2 (moderate) and 0 (no infection), respectively.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Although there was a marked decrease in cfu number, signs of biofilm-induced infection prevailed after 14\u2009days of treatment. Further studies should be carried out to evaluate the potential of dalbavancin in the treatment of bone and orthopaedic implant-associated MRSA infections.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkaa163","type":"journal-article","created":{"date-parts":[[2020,4,6]],"date-time":"2020-04-06T19:10:46Z","timestamp":1586200246000},"page":"2182-2187","source":"Crossref","is-referenced-by-count":19,"title":["Efficacy of dalbavancin against MRSA biofilms in a rat model of orthopaedic implant-associated infection"],"prefix":"10.1093","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9406-8433","authenticated-orcid":false,"given":"Vanessa","family":"Silva","sequence":"first","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Vila Real, Portugal"},{"name":"Department of Genetics and Biotechnology, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Vila Real, Portugal"},{"name":"Functional Genomics and Proteomics Unit, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Vila Real, Portugal"},{"name":"Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), University NOVA of Lisboa, Lisboa, Caparica, Portugal"}]},{"given":"H Sofia","family":"Ant\u00e3o","sequence":"additional","affiliation":[{"name":"Angelini, Medical Department, C. Quebrada-Dafundo, Portugal"}]},{"given":"Jo\u00e3o","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Angelini, Medical Department, C. Quebrada-Dafundo, Portugal"}]},{"given":"Justina","family":"Prada","sequence":"additional","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Vila Real, Portugal"},{"name":"CECAV, Vila Real, Portugal"}]},{"given":"Isabel","family":"Pires","sequence":"additional","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Vila Real, Portugal"},{"name":"Department of Zootechnics, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Vila Real, Portugal"}]},{"given":"\u00c2ngela","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Zootechnics, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Vila Real, Portugal"}]},{"given":"Lu\u00eds","family":"Maltez","sequence":"additional","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Vila Real, Portugal"},{"name":"CECAV, Vila Real, Portugal"}]},{"given":"Jos\u00e9 E","family":"Pereira","sequence":"additional","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Vila Real, Portugal"},{"name":"CECAV, Vila Real, Portugal"}]},{"given":"Jos\u00e9 L","family":"Capelo","sequence":"additional","affiliation":[{"name":"BIOSCOPE Group, LAQV@REQUIMTE, Chemistry Department, Faculty of Science and Technology, NOVA University of Lisbon, Almada, Portugal"},{"name":"Proteomass Scientific Society, Costa de Caparica, Portugal"}]},{"given":"Gilberto","family":"Igrejas","sequence":"additional","affiliation":[{"name":"Department of Genetics and Biotechnology, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Vila Real, Portugal"},{"name":"Functional Genomics and Proteomics Unit, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Vila Real, Portugal"},{"name":"Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), University NOVA of Lisboa, Lisboa, Caparica, Portugal"}]},{"given":"Patr\u00edcia","family":"Poeta","sequence":"additional","affiliation":[{"name":"Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes and Alto Douro (UTAD), Vila Real, Portugal"},{"name":"Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), University NOVA of Lisboa, Lisboa, Caparica, Portugal"}]}],"member":"286","published-online":{"date-parts":[[2020,5,17]]},"reference":[{"key":"2021051700090780900_dkaa163-B1","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.3389\/fmicb.2018.01485","article-title":"Intra-hospital, inter-hospital and intercontinental spread of ST78 MRSA from two neonatal intensive care unit outbreaks established using whole-genome sequencing","volume":"9","author":"Earls","year":"2018","journal-title":"Front Microbiol"},{"key":"2021051700090780900_dkaa163-B2","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1186\/s12917-018-1611-0","article-title":"Investigation of methicillin-resistant Staphylococcus aureus among clinical isolates from humans and animals by culture methods and multiplex PCR","volume":"14","author":"Rahman","year":"2018","journal-title":"BMC Vet Res"},{"key":"2021051700090780900_dkaa163-B3","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1002\/jor.20943","article-title":"Biofilm dispersal of community-associated methicillin-resistant Staphylococcus aureus on orthopedic implant material","volume":"28","author":"Lauderdale","year":"2009","journal-title":"J Orthop Res"},{"key":"2021051700090780900_dkaa163-B4","doi-asserted-by":"crossref","first-page":"3646","DOI":"10.1016\/j.jmb.2015.09.002","article-title":"The limitations of in vitro experimentation in understanding biofilms and chronic infection","volume":"427","author":"Roberts","year":"2015","journal-title":"J Mol Biol"},{"key":"2021051700090780900_dkaa163-B5","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.ijantimicag.2009.12.011","article-title":"Antibiotic resistance of bacterial biofilms","volume":"35","author":"H\u00f8iby","year":"2010","journal-title":"Int J Antimicrob Agents"},{"key":"2021051700090780900_dkaa163-B6","doi-asserted-by":"crossref","first-page":"5286","DOI":"10.3390\/molecules20045286","article-title":"Antibiotic discovery: combatting bacterial resistance in cells and in biofilm communities","volume":"20","author":"Penesyan","year":"2015","journal-title":"Molecules"},{"key":"2021051700090780900_dkaa163-B7","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.tim.2014.02.001","article-title":"Molecular mechanisms of antimicrobial tolerance and resistance in bacterial and fungal biofilms","volume":"22","author":"Van Acker","year":"2014","journal-title":"Trends Microbiol"},{"key":"2021051700090780900_dkaa163-B8","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.5114\/aoms.2016.59838","article-title":"New agents approved for treatment of acute staphylococcal skin infections","volume":"12","author":"Tatarkiewicz","year":"2016","journal-title":"Arch Med Sci"},{"key":"2021051700090780900_dkaa163-B9","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.diagmicrobio.2016.05.009","article-title":"In vitro activity of dalbavancin against biofilms of staphylococci isolated from prosthetic joint infections","volume":"85","author":"Fern\u00e1ndez","year":"2016","journal-title":"Diagn Microbiol Infect Dis"},{"key":"2021051700090780900_dkaa163-B10","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1007\/s10096-016-2845-z","article-title":"Dalbavancin reduces biofilms of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus epidermidis (MRSE)","volume":"36","author":"Knafl","year":"2017","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"2021051700090780900_dkaa163-B11","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.diagmicrobio.2017.09.015","article-title":"Dalbavancin is active in vitro against biofilms formed by dalbavancin-susceptible enterococci","volume":"90","author":"Neudorfer","year":"2018","journal-title":"Diagn Microbiol Infect Dis"},{"key":"2021051700090780900_dkaa163-B12","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.jgar.2019.05.017","article-title":"First report of linezolid-resistant cfr-positive methicillin-resistant Staphylococcus aureus in humans in Portugal","volume":"17","author":"Silva","year":"2019","journal-title":"J Glob Antimicrob Resist"},{"key":"2021051700090780900_dkaa163-B13","doi-asserted-by":"crossref","first-page":"1289157","DOI":"10.1155\/2016\/1289157","article-title":"In vitro and in vivo biofilm characterization of methicillin-resistant Staphylococcus aureus from patients associated with pharyngitis infection","volume":"2016","author":"Gowrishankar","year":"2016","journal-title":"Biomed Res Int"},{"key":"2021051700090780900_dkaa163-B14","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1006\/jsre.1998.5416","article-title":"Application of a rat osteomyelitis model to compare in vivo and in vitro the antibiotic efficacy against bacteria with high capacity to form biofilms","volume":"79","author":"Gracia","year":"1998","journal-title":"J Surg Res"},{"key":"2021051700090780900_dkaa163-B15","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1002\/jor.24230","article-title":"Recommendations for design and conduct of preclinical in vivo studies of orthopedic device-related infection","volume":"37","author":"Moriarty","year":"2019","journal-title":"J Orthop Res"},{"key":"2021051700090780900_dkaa163-B16","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.2106\/00004623-198567080-00015","article-title":"The influence of skeletal implants on incidence of infection. Experiments in a canine model","volume":"67","author":"Petty","year":"1985","journal-title":"J Bone Joint Surg Am"},{"key":"2021051700090780900_dkaa163-B17","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1177\/039139880502801103","article-title":"Biofilm in implant infections: its production and regulation","volume":"28","author":"Costerton","year":"2005","journal-title":"Int J Artif Organs"},{"key":"2021051700090780900_dkaa163-B18","doi-asserted-by":"crossref","first-page":"9661","DOI":"10.1038\/s41598-018-28006-8","article-title":"Dalbavancin for treatment of implant-related methicillin-resistant Staphylococcus aureus osteomyelitis in an experimental rat model","volume":"8","author":"Kussmann","year":"2018","journal-title":"Sci Rep"},{"key":"2021051700090780900_dkaa163-B19","doi-asserted-by":"crossref","first-page":"4999","DOI":"10.1128\/AAC.00584-11","article-title":"Daptomycin, fosfomycin, or both for treatment of methicillin-resistant Staphylococcus aureus osteomyelitis in an experimental rat model","volume":"55","author":"Poeppl","year":"2011","journal-title":"Antimicrob Agents Chemother"},{"key":"2021051700090780900_dkaa163-B20","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1016\/S0736-0266(00)00076-0","article-title":"A simple infection model using pre-colonized implants to reproduce rat chronic Staphylococcus aureus osteomyelitis and study antibiotic treatment","volume":"19","author":"Monz\u00f3n","year":"2001","journal-title":"J Orthop Res"},{"key":"2021051700090780900_dkaa163-B21","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1177\/039139880502801106","article-title":"Etiology of implant orthopedic infections: a survey on 1027 clinical isolates","volume":"28","author":"Arciola","year":"2005","journal-title":"Int J Artif Organs"},{"key":"2021051700090780900_dkaa163-B22","doi-asserted-by":"crossref","first-page":"5967","DOI":"10.1016\/j.biomaterials.2012.05.031","article-title":"Biofilm formation in Staphylococcus implant infections. A review of molecular mechanisms and implications for biofilm-resistant materials","volume":"33","author":"Arciola","year":"2012","journal-title":"Biomaterials"},{"key":"2021051700090780900_dkaa163-B23","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1097\/00003086-200508000-00011","article-title":"Engineering approaches for the detection and control of orthopaedic biofilm infections","volume":"437","author":"Ehrlich","year":"2005","journal-title":"Clin Orthop Relat Res"},{"key":"2021051700090780900_dkaa163-B24","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.diagmicrobio.2018.10.007","article-title":"Dalbavancin for the management of gram-positive osteomyelitis: effectiveness and potential utility","volume":"93","author":"Almangour","year":"2019","journal-title":"Diagn Microbiol Infect Dis"},{"key":"2021051700090780900_dkaa163-B25","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1080\/1120009X.2017.1404277","article-title":"The role of dalbavancin in the multi-disciplinary management of wound infections in orthopaedic surgery","volume":"30","author":"Arena","year":"2018","journal-title":"J Chemother"},{"key":"2021051700090780900_dkaa163-B26","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1093\/jac\/dkv357","article-title":"Efficacy of dalbavancin in the treatment of MRSA rat sternal osteomyelitis with mediastinitis","volume":"71","author":"Barnea","year":"2016","journal-title":"J Antimicrob Chemother"},{"key":"2021051700090780900_dkaa163-B27","doi-asserted-by":"crossref","first-page":"e01067","DOI":"10.1016\/j.heliyon.2018.e01067","article-title":"Bacterial biofilm formation on implantable devices and approaches to its treatment and prevention","volume":"4","author":"Khatoon","year":"2018","journal-title":"Heliyon"},{"key":"2021051700090780900_dkaa163-B28","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1177\/0091270008321273","article-title":"Pharmacokinetic-pharmacodynamic modeling of dalbavancin, a novel glycopeptide antibiotic","volume":"48","author":"Dowell","year":"2008","journal-title":"J Clin Pharmacol"},{"key":"2021051700090780900_dkaa163-B29","doi-asserted-by":"crossref","first-page":"1849","DOI":"10.1128\/AAC.04550-14","article-title":"Extended-duration dosing and distribution of dalbavancin into bone and articular tissue","volume":"59","author":"Dunne","year":"2015","journal-title":"Antimicrob Agents Chemother"}],"container-title":["Journal of Antimicrobial Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jac\/article-pdf\/75\/8\/2182\/33505518\/dkaa163.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jac\/article-pdf\/75\/8\/2182\/33505518\/dkaa163.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T00:09:37Z","timestamp":1621210177000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jac\/article\/75\/8\/2182\/5838314"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,17]]},"references-count":29,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2020,5,17]]},"published-print":{"date-parts":[[2020,8,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jac\/dkaa163","relation":{},"ISSN":["0305-7453","1460-2091"],"issn-type":[{"value":"0305-7453","type":"print"},{"value":"1460-2091","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,8]]},"published":{"date-parts":[[2020,5,17]]}}}