{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T15:45:34Z","timestamp":1775576734193,"version":"3.50.1"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Applied Molecular Biosciences Unit\u2014UCIBIO"},{"DOI":"10.13039\/100006129","name":"FCT","doi-asserted-by":"publisher","award":["UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDB\/04378\/2020"]}],"id":[{"id":"10.13039\/100006129","id-type":"DOI","asserted-by":"publisher"}]},{"name":"QREN","award":["NORTE-01-0145-FEDER-000024 211"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-000024 211"]}]},{"name":"QREN","award":["NORTE-01-0145-FEDER-00001"],"award-info":[{"award-number":["NORTE-01-0145-FEDER-00001"]}]},{"DOI":"10.13039\/100006129","name":"FCT","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006111","name":"MCTES","doi-asserted-by":"publisher","award":["CD018\/0123"],"award-info":[{"award-number":["CD018\/0123"]}],"id":[{"id":"10.13039\/501100006111","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Development Regional Fund"},{"name":"Tunisian Ministry of Higher Education and Scientific Research"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>poxtA is the most recently described gene conferring acquired resistance to linezolid, a relevant antibiotic for treating enterococcal infections. We retrospectively screened for poxtA in diverse enterococci and aimed to characterize its genetic\/genomic contexts.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>poxtA was screened by PCR in 812 enterococci from 458 samples (hospitals\/healthy humans\/wastewater\/animals\/retail food) obtained in Portugal\/Angola\/Tunisia (1996\u20132019). Antimicrobial susceptibility testing was performed for 13 antibiotics (EUCAST\/CLSI). poxtA stability (\u223c500 generations), transfer (filter mating), clonality (SmaI-PFGE) and location (S1-PFGE\/hybridization) were tested. WGS (Illumina-HiSeq) was performed for clonal representatives.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>poxtA was detected in Enterococcus faecium from six samples (1.3%): a healthy human (rectal swab) in Porto, Portugal (ST32\/2001); four farm cows (milk) in Mateur, Tunisia (ST1058\/2015); and a hospitalized patient (faeces) in Matosinhos, Portugal (ST1058\/2015). All expressed resistance to linezolid (MIC\u2009=\u20098\u2009mg\/L), chloramphenicol, tetracycline and erythromycin, with variable resistance to ciprofloxacin and streptomycin. ST1058-poxtA-carrying isolates from Tunisia and Portugal differed by two SNPs and had similar plasmid content. poxtA, located in an IS1216-flanked Tn6246-like element, co-hybridized with fexB on one or more plasmids per isolate (one to three plasmids of 30\u2013100\u2009kb), was stable after several generations and transferred only from ST1058. ST1058 strains carried resistance\/virulence genes (Efmqnr\/acm) possibly induced under selective quinolone treatment.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>poxtA has been circulating in Portugal since at least 2001, corresponding to the oldest description worldwide to date. We also extend the reservoir of poxtA to bovines. The similar linezolid-resistant poxtA-carrying strains colonizing humans and livestock on different continents, and without a noticeable relationship, suggests a recent transmission event or convergent evolution of E. faecium populations in different hosts and geographic regions.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkaa227","type":"journal-article","created":{"date-parts":[[2020,5,12]],"date-time":"2020-05-12T03:11:00Z","timestamp":1589253060000},"page":"2416-2423","source":"Crossref","is-referenced-by-count":48,"title":["Linezolid-resistant (Tn<i>6246<\/i>::<i>fexB<\/i>-<i>poxtA<\/i>) <i>Enterococcus faecium<\/i> strains colonizing humans and bovines on different continents: similarity without epidemiological link"],"prefix":"10.1093","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7326-4133","authenticated-orcid":false,"given":"Ana R","family":"Freitas","sequence":"first","affiliation":[{"name":"UCIBIO\/REQUIMTE, Departamento de Ci\u00eancias Biol\u00f3gicas, Laborat\u00f3rio de Microbiologia, Faculdade de Farm\u00e1cia, Universidade do Porto, Porto, Portugal"}]},{"given":"Ana P","family":"Tedim","sequence":"additional","affiliation":[{"name":"UCIBIO\/REQUIMTE, Departamento de Ci\u00eancias Biol\u00f3gicas, Laborat\u00f3rio de Microbiologia, Faculdade de Farm\u00e1cia, Universidade do Porto, Porto, Portugal"},{"name":"Grupo de Investigaci\u00f3n Biom\u00e9dica en Sepsis \u2013 BioSepsis, Hospital Universitario Rio Hortega\/Instituto de Investigaci\u00f3n Biom\u00e9dica de Salamanca (IBSAL), Valladolid\/Salamanca, Spain"}]},{"given":"B\u00e1rbara","family":"Duarte","sequence":"additional","affiliation":[{"name":"UCIBIO\/REQUIMTE, Departamento de Ci\u00eancias Biol\u00f3gicas, Laborat\u00f3rio de Microbiologia, Faculdade de Farm\u00e1cia, Universidade do Porto, Porto, Portugal"},{"name":"Departamento de Ci\u00eancias Biol\u00f3gicas, Unidade de An\u00e1lises Cl\u00ednicas, Faculdade de Farm\u00e1cia, Universidade do Porto, Porto, Portugal"}]},{"given":"Houyem","family":"Elghaieb","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Tunis El Manar, Institut de la Recherche V\u00e9t\u00e9rinaire de Tunisie, 20 rue Jebel Lakhdhar, Bab Saadoun, Tunis, Tunisia"}]},{"given":"Mohamed S","family":"Abbassi","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Tunis El Manar, Institut de la Recherche V\u00e9t\u00e9rinaire de Tunisie, 20 rue Jebel Lakhdhar, Bab Saadoun, Tunis, Tunisia"}]},{"given":"Abdennaceur","family":"Hassen","sequence":"additional","affiliation":[{"name":"Laboratoire de Traitement des Eaux Us\u00e9es, Centre des Recherches et des Technologies des Eaux (CERTE), Technopole Borj C\u00e9dria, Soliman, Tunisia"}]},{"given":"Ant\u00f3nia","family":"Read","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Patologia Cl\u00ednica-Microbiologia, Hospital Pedro Hispano, Matosinhos, Portugal"}]},{"given":"Valqu\u00edria","family":"Alves","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Patologia Cl\u00ednica-Microbiologia, Hospital Pedro Hispano, Matosinhos, Portugal"}]},{"given":"Carla","family":"Novais","sequence":"additional","affiliation":[{"name":"UCIBIO\/REQUIMTE, Departamento de Ci\u00eancias Biol\u00f3gicas, Laborat\u00f3rio de Microbiologia, Faculdade de Farm\u00e1cia, Universidade do Porto, Porto, Portugal"}]},{"given":"Lu\u00edsa","family":"Peixe","sequence":"additional","affiliation":[{"name":"UCIBIO\/REQUIMTE, Departamento de Ci\u00eancias Biol\u00f3gicas, Laborat\u00f3rio de Microbiologia, Faculdade de Farm\u00e1cia, Universidade do Porto, Porto, Portugal"}]}],"member":"286","published-online":{"date-parts":[[2020,7,1]]},"reference":[{"key":"2021070100034195400_dkaa227-B1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.drup.2018.10.002","article-title":"Update on prevalence and mechanisms of resistance to linezolid, 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