{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T18:17:34Z","timestamp":1777141054119,"version":"3.51.4"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T00:00:00Z","timestamp":1748649600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015621","name":"UCIBIO","doi-asserted-by":"publisher","award":["UIDP\/04378\/2020"],"award-info":[{"award-number":["UIDP\/04378\/2020"]}],"id":[{"id":"10.13039\/501100015621","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015621","name":"UCIBIO","doi-asserted-by":"publisher","award":["UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDB\/04378\/2020"]}],"id":[{"id":"10.13039\/501100015621","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015750","name":"Associate Laboratory Institute for Health and Bioeconomy","doi-asserted-by":"publisher","award":["LA\/P\/0140\/2020"],"award-info":[{"award-number":["LA\/P\/0140\/2020"]}],"id":[{"id":"10.13039\/501100015750","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Expansion of carbapenemase-producing Klebsiella pneumoniae (CP-Kp) is driven by within-hospital transmission, requiring timely typing data for effective infection control.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>We evaluated real-time performance and flexibility of our previously developed Fourier-transform infrared (FT-IR) spectroscopy workflow (spectra acquisition and analysis by machine-learning model).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>All CP-Kp infection isolates (n\u200a=\u200a136) identified at a northern Portuguese hospital (April 2022\u2014March 2023) were tested from Columbia agar with 5% sheep blood, identified by FT-IR (KL-type\/sublineage) and confirmed by reference methods (wzi sequencing, MLST and\/or WGS).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>FT-IR typing from Columbia agar with 5% sheep blood showed 73% sensitivity, 79% specificity and 74% accuracy. Our method correctly identified 94% of typeable isolates, 87% of which were communicated in &amp;lt;24\u2005h. Non-typeable isolates belonged to new KL-types to the model (40%) or non-recognized KL-types (60%), most of which (66%) were correctly predicted when retested from Mueller\u2013Hinton agar. Accuracy was then higher (88%) when results from both culture media were considered, and the model retrained to incorporate new sublineages. Three K. pneumoniae sublineages (ST147-KL64, ST15-KL19, ST268-KL20) were predominant and 86% of the isolates were correctly identified. During the study, an outbreak by ST268-KL20 in the neonatal ICU was quickly recognized, and solved in 23\u2005days. Most isolates (98%) produced KPC-3.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>We demonstrate that FT-IR spectroscopy meets high performance standards in real-time and adaptability to clonal dynamics, and we provide practical guidance for integrating FT-IR into daily microbiology practices. The unique time to response (same day as bacterial identification) enables early and effective infection control interventions.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkaf170","type":"journal-article","created":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T10:57:02Z","timestamp":1748689022000},"page":"2025-2031","source":"Crossref","is-referenced-by-count":3,"title":["Real-time FT-IR typing of <i>Klebsiella pneumoniae<\/i>: a flexible and rapid approach for outbreak detection and infection control"],"prefix":"10.1093","volume":"80","author":[{"given":"Ana Beatriz","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"UCIBIO, i4HB, Faculdade de Farm\u00e1cia da Universidade do Porto , Rua Jorge Viterbo Ferreira 228, Porto 4050-313 ,","place":["Portugal"]}]},{"given":"Valqu\u00edria","family":"Alves","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Local Healthcare Unit , Matosinhos 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