{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T05:45:49Z","timestamp":1773812749286,"version":"3.50.1"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>The present study was designed to provide a snapshot on carbapenemase-producing Pseudomonas aeruginosa (n\u200a=\u200a11) and Acinetobacter baumannii (n\u200a=\u200a7) isolates in hospitalized patients (November 2011, January\u2013March 2012) from two main hospitals in Bucharest, south Romania.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Clonality among isolates was established by PFGE, MLST and Fourier transform infrared spectroscopy. Carbapenemases were screened by the Blue-Carba test, PCR and sequencing. Transferability of blaOXA-23 was tested by conjugation and plasmid typing (number, size\u00a0and identity) was assessed by S1-PFGE, replicon typing, hybridization and PCR mapping.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>All P. aeruginosa isolates carried chromosomally located blaVIM-2, associated with a common class 1 integron (aacA7-blaVIM-2) or an atypical configuration (aacA7-blaVIM-2-dfrB5-tniC). These isolates belonged to unusual lineages; mostly ST233 disseminated in one hospital unit, with ST364 and ST1074 also being detected. A. baumannii isolates carried blaOXA-23 in Tn2008, which was found truncating a TnaphA6 transposon located in a common 60 kb GR6 (aci6) pABKp1-like conjugative plasmid in highly related CC92 clones (ST437, ST764 and ST765), where CC stands for clonal complex.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our results show the spread of VIM-2-producing P. aeruginosa and OXA-23-producing A. baumannii clinical isolates in two hospitals from Bucharest and highlight a peculiar population structure in this Eastern European country. Also, we demonstrate the dissemination of a common and conjugative aci6 pABKp1-like plasmid scaffold in different A. baumannii clones and we report the first known identification of Tnaph6-carrying pACICU2-like plasmids in Europe.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dku527","type":"journal-article","created":{"date-parts":[[2015,1,29]],"date-time":"2015-01-29T04:31:38Z","timestamp":1422505898000},"page":"1016-1020","source":"Crossref","is-referenced-by-count":27,"title":["Snapshot on carbapenemase-producing <i>Pseudomonas aeruginosa<\/i> and <i>Acinetobacter baumannii<\/i> in Bucharest hospitals reveals unusual clones and novel genetic surroundings for <i>bla<\/i>OXA-23"],"prefix":"10.1093","volume":"70","author":[{"given":"Irina","family":"Gheorghe","sequence":"first","affiliation":[{"name":"1 \u00a0Department of Microbiology, Faculty of Biology, University of Bucharest, Bucharest, Romania"}]},{"given":"\u00c2ngela","family":"Novais","sequence":"additional","affiliation":[{"name":"2 \u00a0UCIBIO\/REQUIMTE, Laborat\u00f3rio de Microbiologia, Faculdade de Farm\u00e1cia, Universidade do Porto, Porto, Portugal"}]},{"given":"Filipa","family":"Grosso","sequence":"additional","affiliation":[{"name":"2 \u00a0UCIBIO\/REQUIMTE, Laborat\u00f3rio de Microbiologia, Faculdade de Farm\u00e1cia, Universidade do Porto, Porto, Portugal"}]},{"given":"Carla","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"2 \u00a0UCIBIO\/REQUIMTE, Laborat\u00f3rio de Microbiologia, Faculdade de Farm\u00e1cia, Universidade do Porto, Porto, Portugal"}]},{"given":"Mariana Carmen","family":"Chifiriuc","sequence":"additional","affiliation":[{"name":"1 \u00a0Department of Microbiology, Faculty of Biology, University of Bucharest, Bucharest, Romania"}]},{"given":"Veronica","family":"Lazar","sequence":"additional","affiliation":[{"name":"1 \u00a0Department of Microbiology, Faculty of Biology, University of Bucharest, Bucharest, Romania"}]},{"given":"Lu\u00edsa","family":"Peixe","sequence":"additional","affiliation":[{"name":"2 \u00a0UCIBIO\/REQUIMTE, Laborat\u00f3rio de Microbiologia, Faculdade de Farm\u00e1cia, Universidade do Porto, Porto, Portugal"}]}],"member":"286","published-online":{"date-parts":[[2015,1,27]]},"reference":[{"key":"2022020120544553900_DKU527C1","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1093\/jac\/dku048","article-title":"Epidemiology and carbapenem resistance mechanisms of carbapenem-non-susceptible Pseudomonas aeruginosa collected during 2009-11 in 14 European and Mediterranean countries","volume":"69","author":"Castanheira","year":"2014","journal-title":"J Antimicrob Chemother"},{"key":"2022020120544553900_DKU527C2","doi-asserted-by":"crossref","first-page":"2820","DOI":"10.1093\/jac\/dkt269","article-title":"Dissemination of metallo-\u03b2-lactamase-producing Pseudomonas aeruginosa of sequence type 235 in Asian 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