{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T16:20:05Z","timestamp":1771258805766,"version":"3.50.1"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2024,6,15]],"date-time":"2024-06-15T00:00:00Z","timestamp":1718409600000},"content-version":"vor","delay-in-days":14,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Galician Competitive Research Group","award":["ED431C-2021\/37"],"award-info":[{"award-number":["ED431C-2021\/37"]}]},{"name":"Juan de la Cierva-Formaci\u00f3n","award":["FJC2019-041664-I"],"award-info":[{"award-number":["FJC2019-041664-I"]}]},{"name":"Histology and Electron Microscopy","award":["PPBI-POCI-01\u20130145-FEDER-022122"],"award-info":[{"award-number":["PPBI-POCI-01\u20130145-FEDER-022122"]}]},{"name":"Histology and Electron Microscopy","award":["UIDB\/00\u00a0511\/2020"],"award-info":[{"award-number":["UIDB\/00\u00a0511\/2020"]}]},{"name":"Histology and Electron Microscopy","award":["UIDP\/00\u00a0511\/2020"],"award-info":[{"award-number":["UIDP\/00\u00a0511\/2020"]}]},{"name":"Histology and Electron Microscopy","award":["LA\/P\/0045\/2020"],"award-info":[{"award-number":["LA\/P\/0045\/2020"]}]},{"DOI":"10.13039\/501100001871","name":"FCT","doi-asserted-by":"publisher","award":["10.54499\/2020.04773.BD"],"award-info":[{"award-number":["10.54499\/2020.04773.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006111","name":"MCTES","doi-asserted-by":"publisher","award":["UIDB\/04469\/2020"],"award-info":[{"award-number":["UIDB\/04469\/2020"]}],"id":[{"id":"10.13039\/501100006111","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006111","name":"MCTES","doi-asserted-by":"publisher","award":["LA\/P\/0029\/2020"],"award-info":[{"award-number":["LA\/P\/0029\/2020"]}],"id":[{"id":"10.13039\/501100006111","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006111","name":"MCTES","doi-asserted-by":"publisher","award":["UIDB\/04033\/2020"],"award-info":[{"award-number":["UIDB\/04033\/2020"]}],"id":[{"id":"10.13039\/501100006111","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Aim<\/jats:title>\n                  <jats:p>Coaggregation, a highly specific cell\u2013cell interaction mechanism, plays a pivotal role in multispecies biofilm formation. While it has been mostly studied in oral environments, its occurrence in aquatic systems is also acknowledged. Considering biofilm formation\u2019s economic and health-related implications in engineered water systems, it is crucial to understand its mechanisms. Here, we hypothesized that traceable differences at the proteome level might determine coaggregation ability.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and Results<\/jats:title>\n                  <jats:p>Two strains of Delftia acidovorans, isolated from drinking water were studied. First, in vitro motility assays indicated more swarming and twitching motility for the coaggregating strain (C+) than non-coaggregating strain (C\u2212). By transmission electronic microscopy, we confirmed the presence of flagella for both strains. By proteomics, we detected a significantly higher expression of type IV pilus twitching motility proteins in C+, in line with the motility assays. Moreover, flagellum ring proteins were more abundant in C+, while those involved in the formation of the flagellar hook (FlE and FilG) were only detected in C\u2212. All the results combined suggested structural and conformational differences between stains in their cell appendages.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>This study presents an alternative approach for identifying protein biomarkers to detect coaggregation abilities in uncharacterized strains.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jambio\/lxae143","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T16:36:49Z","timestamp":1718383009000},"source":"Crossref","is-referenced-by-count":4,"title":["Exploring coaggregation mechanisms involved in biofilm formation in drinking water through a proteomic-based approach"],"prefix":"10.1093","volume":"135","author":[{"given":"Ana C","family":"Afonso","sequence":"first","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto , Rua Dr Roberto Frias, 4200-465 Porto , Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto , Rua Dr Roberto Frias, 4200-465 Porto , Portugal"},{"name":"CITAB, Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes e Alto Douro , 5000-801 Vila Real , Portugal"},{"name":"CEB-LABBELS, University of Minho, Campus de Gualtar , 4710-057 Braga , Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3355-4398","authenticated-orcid":false,"given":"Manuel","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"LEPABE\u2014Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto , Rua Dr Roberto Frias, 4200-465 Porto , Portugal"},{"name":"ALiCE\u2014Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto , Rua Dr Roberto Frias, 4200-465 Porto , Portugal"}]},{"given":"Maria Jos\u00e9","family":"Saavedra","sequence":"additional","affiliation":[{"name":"CITAB, Department of Veterinary Sciences, University of Tr\u00e1s-os-Montes e Alto Douro , 5000-801 Vila Real , Portugal"}]},{"given":"L\u00facia","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"CEB-LABBELS, University of Minho, Campus de Gualtar , 4710-057 Braga , Portugal"}]},{"given":"Juan M","family":"Lema","sequence":"additional","affiliation":[{"name":"CRETUS, Department of Chemical Engineering, University of Santiago de Compostela, Campus Vida , 15782 Santiago de Compostela, Galicia , Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9730-7321","authenticated-orcid":false,"given":"Alba","family":"Trueba-Santiso","sequence":"additional","affiliation":[{"name":"CRETUS, Department of Chemical Engineering, University of Santiago de Compostela, Campus Vida , 15782 Santiago de Compostela, Galicia , 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